Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_0550 and Shewana3_0551 are separated by 6 nucleotides Shewana3_0551 and Shewana3_0552 are separated by 149 nucleotides Shewana3_0552 and Shewana3_0553 are separated by 272 nucleotides Shewana3_0553 and Shewana3_0554 are separated by 15 nucleotides
Shewana3_0550: Shewana3_0550 - hypothetical protein (RefSeq), at 644,599 to 645,108
_0550
Shewana3_0551: Shewana3_0551 - hypothetical protein (RefSeq), at 645,115 to 645,429
_0551
Shewana3_0552: Shewana3_0552 - hypothetical protein (RefSeq), at 645,579 to 645,887
_0552
Shewana3_0553: Shewana3_0553 - hypothetical protein (RefSeq), at 646,160 to 646,684
_0553
Shewana3_0554: Shewana3_0554 - diguanylate cyclase/phosphodiesterase (RefSeq), at 646,700 to 648,454
_0554
Position (kb) 645
646 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 644.843 kb on + strand, within Shewana3_0550 at 644.898 kb on + strand, within Shewana3_0550 at 644.924 kb on + strand, within Shewana3_0550 at 645.151 kb on + strand, within Shewana3_0551 at 645.172 kb on + strand, within Shewana3_0551 at 645.239 kb on + strand, within Shewana3_0551 at 645.239 kb on + strand, within Shewana3_0551 at 645.314 kb on + strand, within Shewana3_0551 at 645.314 kb on + strand, within Shewana3_0551 at 645.322 kb on - strand, within Shewana3_0551 at 645.362 kb on - strand, within Shewana3_0551 at 645.362 kb on - strand, within Shewana3_0551 at 645.707 kb on + strand, within Shewana3_0552 at 645.707 kb on + strand, within Shewana3_0552 at 645.715 kb on - strand, within Shewana3_0552 at 646.198 kb on - strand at 646.203 kb on + strand at 646.508 kb on + strand, within Shewana3_0553
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 644,843 + Shewana3_0550 0.48 +0.1 644,898 + Shewana3_0550 0.59 -0.6 644,924 + Shewana3_0550 0.64 -0.7 645,151 + Shewana3_0551 0.11 -0.3 645,172 + Shewana3_0551 0.18 -1.9 645,239 + Shewana3_0551 0.39 +0.2 645,239 + Shewana3_0551 0.39 -0.8 645,314 + Shewana3_0551 0.63 -3.8 645,314 + Shewana3_0551 0.63 -0.6 645,322 - Shewana3_0551 0.66 -1.0 645,362 - Shewana3_0551 0.78 -0.9 645,362 - Shewana3_0551 0.78 -0.4 645,707 + Shewana3_0552 0.41 -3.4 645,707 + Shewana3_0552 0.41 -1.8 645,715 - Shewana3_0552 0.44 -2.2 646,198 - +1.8 646,203 + +0.4 646,508 + Shewana3_0553 0.66 -0.6
Or see this region's nucleotide sequence