Experiment: L-Leucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_508 and Pf1N1B4_509 are separated by 92 nucleotides Pf1N1B4_509 and Pf1N1B4_510 overlap by 25 nucleotides Pf1N1B4_510 and Pf1N1B4_511 are separated by 60 nucleotides Pf1N1B4_511 and Pf1N1B4_512 are separated by 105 nucleotides
Pf1N1B4_508 - Methyl-accepting chemotaxis protein I (serine chemoreceptor protein), at 599,543 to 601,495
_508
Pf1N1B4_509 - hypothetical protein, at 601,588 to 601,710
_509
Pf1N1B4_510 - Transcriptional regulator, GntR family domain / Aspartate aminotransferase (EC 2.6.1.1), at 601,686 to 603,065
_510
Pf1N1B4_511 - hypothetical protein, at 603,126 to 603,254
_511
Pf1N1B4_512 - Short-chain dehydrogenase/reductase SDR, at 603,360 to 604,121
_512
Position (kb) 601
602
603
604 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 600.735 kb on + strand, within Pf1N1B4_508 at 600.736 kb on - strand, within Pf1N1B4_508 at 600.915 kb on - strand, within Pf1N1B4_508 at 600.915 kb on - strand, within Pf1N1B4_508 at 601.021 kb on + strand, within Pf1N1B4_508 at 601.021 kb on + strand, within Pf1N1B4_508 at 601.021 kb on + strand, within Pf1N1B4_508 at 601.021 kb on + strand, within Pf1N1B4_508 at 601.022 kb on - strand, within Pf1N1B4_508 at 601.022 kb on - strand, within Pf1N1B4_508 at 601.022 kb on - strand, within Pf1N1B4_508 at 601.023 kb on + strand, within Pf1N1B4_508 at 601.023 kb on + strand, within Pf1N1B4_508 at 601.023 kb on + strand, within Pf1N1B4_508 at 601.023 kb on + strand, within Pf1N1B4_508 at 601.024 kb on - strand, within Pf1N1B4_508 at 601.024 kb on - strand, within Pf1N1B4_508 at 601.024 kb on - strand, within Pf1N1B4_508 at 601.024 kb on - strand, within Pf1N1B4_508 at 601.398 kb on - strand at 601.398 kb on - strand at 601.439 kb on + strand at 601.440 kb on - strand at 601.514 kb on + strand at 601.587 kb on - strand at 601.741 kb on + strand at 601.741 kb on + strand at 601.741 kb on + strand at 601.741 kb on + strand at 601.742 kb on - strand at 601.746 kb on + strand at 601.746 kb on + strand at 601.746 kb on + strand at 601.746 kb on + strand at 601.746 kb on + strand at 601.746 kb on + strand at 601.746 kb on + strand at 601.747 kb on - strand at 601.747 kb on - strand at 601.747 kb on - strand at 601.747 kb on - strand at 601.747 kb on - strand at 601.747 kb on - strand at 601.747 kb on - strand at 601.747 kb on - strand at 601.747 kb on - strand at 601.747 kb on - strand at 601.747 kb on - strand at 601.747 kb on - strand at 601.747 kb on - strand at 601.747 kb on - strand at 601.747 kb on - strand at 601.849 kb on + strand at 601.849 kb on + strand, within Pf1N1B4_510 at 601.850 kb on - strand, within Pf1N1B4_510 at 601.850 kb on - strand at 601.955 kb on + strand, within Pf1N1B4_510 at 601.955 kb on + strand, within Pf1N1B4_510 at 601.955 kb on + strand at 601.956 kb on - strand, within Pf1N1B4_510 at 601.959 kb on + strand, within Pf1N1B4_510 at 601.959 kb on + strand, within Pf1N1B4_510 at 601.960 kb on - strand, within Pf1N1B4_510 at 601.960 kb on - strand, within Pf1N1B4_510 at 601.960 kb on - strand, within Pf1N1B4_510 at 601.960 kb on - strand, within Pf1N1B4_510 at 602.107 kb on - strand, within Pf1N1B4_510 at 602.158 kb on + strand, within Pf1N1B4_510 at 602.158 kb on + strand, within Pf1N1B4_510 at 602.158 kb on + strand, within Pf1N1B4_510 at 602.159 kb on - strand at 602.159 kb on - strand, within Pf1N1B4_510 at 602.159 kb on - strand, within Pf1N1B4_510 at 602.159 kb on - strand, within Pf1N1B4_510 at 602.159 kb on - strand, within Pf1N1B4_510 at 602.159 kb on - strand, within Pf1N1B4_510 at 602.324 kb on - strand, within Pf1N1B4_510 at 602.357 kb on - strand, within Pf1N1B4_510 at 602.357 kb on - strand, within Pf1N1B4_510 at 602.357 kb on - strand, within Pf1N1B4_510 at 602.515 kb on - strand, within Pf1N1B4_510 at 602.644 kb on + strand, within Pf1N1B4_510 at 602.644 kb on + strand, within Pf1N1B4_510 at 602.645 kb on - strand, within Pf1N1B4_510 at 602.685 kb on + strand at 602.685 kb on + strand, within Pf1N1B4_510 at 603.159 kb on - strand, within Pf1N1B4_511 at 603.212 kb on + strand, within Pf1N1B4_511 at 603.212 kb on + strand, within Pf1N1B4_511 at 603.213 kb on - strand, within Pf1N1B4_511 at 603.213 kb on - strand, within Pf1N1B4_511 at 603.223 kb on + strand, within Pf1N1B4_511 at 603.224 kb on - strand, within Pf1N1B4_511 at 603.224 kb on - strand, within Pf1N1B4_511 at 603.224 kb on - strand, within Pf1N1B4_511 at 603.224 kb on - strand at 603.325 kb on + strand at 603.325 kb on + strand at 603.325 kb on + strand at 603.325 kb on + strand at 603.325 kb on + strand at 603.325 kb on + strand at 603.325 kb on + strand at 603.433 kb on + strand at 603.433 kb on + strand at 603.433 kb on + strand at 603.433 kb on + strand at 603.433 kb on + strand at 603.527 kb on - strand, within Pf1N1B4_512 at 603.527 kb on - strand, within Pf1N1B4_512 at 603.652 kb on + strand, within Pf1N1B4_512 at 603.653 kb on - strand, within Pf1N1B4_512 at 603.653 kb on - strand, within Pf1N1B4_512 at 603.653 kb on - strand, within Pf1N1B4_512 at 603.746 kb on - strand, within Pf1N1B4_512 at 603.746 kb on - strand, within Pf1N1B4_512 at 603.862 kb on + strand, within Pf1N1B4_512 at 603.863 kb on - strand at 603.863 kb on - strand, within Pf1N1B4_512 at 603.878 kb on - strand, within Pf1N1B4_512 at 603.882 kb on - strand, within Pf1N1B4_512 at 603.882 kb on - strand, within Pf1N1B4_512 at 603.882 kb on - strand, within Pf1N1B4_512 at 603.882 kb on - strand, within Pf1N1B4_512 at 603.882 kb on - strand, within Pf1N1B4_512 at 603.882 kb on - strand, within Pf1N1B4_512 at 603.882 kb on - strand, within Pf1N1B4_512 at 603.882 kb on - strand, within Pf1N1B4_512
Per-strain Table
Position Strand Gene LocusTag Fraction L-Leucine (N) remove 600,735 + Pf1N1B4_508 0.61 +0.3 600,736 - Pf1N1B4_508 0.61 -1.1 600,915 - Pf1N1B4_508 0.70 +1.5 600,915 - Pf1N1B4_508 0.70 +2.2 601,021 + Pf1N1B4_508 0.76 -1.1 601,021 + Pf1N1B4_508 0.76 -0.3 601,021 + Pf1N1B4_508 0.76 -1.3 601,021 + Pf1N1B4_508 0.76 -0.5 601,022 - Pf1N1B4_508 0.76 +1.9 601,022 - Pf1N1B4_508 0.76 +0.7 601,022 - Pf1N1B4_508 0.76 +1.7 601,023 + Pf1N1B4_508 0.76 -0.8 601,023 + Pf1N1B4_508 0.76 -0.1 601,023 + Pf1N1B4_508 0.76 +0.5 601,023 + Pf1N1B4_508 0.76 -0.1 601,024 - Pf1N1B4_508 0.76 -1.3 601,024 - Pf1N1B4_508 0.76 -0.1 601,024 - Pf1N1B4_508 0.76 -0.5 601,024 - Pf1N1B4_508 0.76 -0.3 601,398 - -0.7 601,398 - +0.3 601,439 + -0.3 601,440 - -0.6 601,514 + -0.1 601,587 - -1.1 601,741 + -0.7 601,741 + -1.1 601,741 + +0.2 601,741 + +1.5 601,742 - +0.5 601,746 + +0.2 601,746 + -0.8 601,746 + +0.9 601,746 + -0.9 601,746 + +1.0 601,746 + +2.2 601,746 + -1.7 601,747 - -0.1 601,747 - +0.9 601,747 - -1.1 601,747 - -0.1 601,747 - -1.1 601,747 - +0.9 601,747 - -1.1 601,747 - +0.9 601,747 - +0.9 601,747 - -0.1 601,747 - -0.7 601,747 - -0.3 601,747 - +0.5 601,747 - +0.3 601,747 - +0.9 601,849 + -1.7 601,849 + Pf1N1B4_510 0.12 -0.1 601,850 - Pf1N1B4_510 0.12 -0.1 601,850 - -1.7 601,955 + Pf1N1B4_510 0.19 -0.3 601,955 + Pf1N1B4_510 0.19 +0.2 601,955 + -1.1 601,956 - Pf1N1B4_510 0.20 -0.2 601,959 + Pf1N1B4_510 0.20 -2.1 601,959 + Pf1N1B4_510 0.20 +0.4 601,960 - Pf1N1B4_510 0.20 -1.5 601,960 - Pf1N1B4_510 0.20 +0.9 601,960 - Pf1N1B4_510 0.20 -1.1 601,960 - Pf1N1B4_510 0.20 +0.5 602,107 - Pf1N1B4_510 0.31 -0.1 602,158 + Pf1N1B4_510 0.34 -1.1 602,158 + Pf1N1B4_510 0.34 -1.1 602,158 + Pf1N1B4_510 0.34 +0.2 602,159 - -1.7 602,159 - Pf1N1B4_510 0.34 -0.2 602,159 - Pf1N1B4_510 0.34 -0.4 602,159 - Pf1N1B4_510 0.34 -1.9 602,159 - Pf1N1B4_510 0.34 +0.2 602,159 - Pf1N1B4_510 0.34 -0.5 602,324 - Pf1N1B4_510 0.46 -2.4 602,357 - Pf1N1B4_510 0.49 +1.0 602,357 - Pf1N1B4_510 0.49 -0.1 602,357 - Pf1N1B4_510 0.49 +0.5 602,515 - Pf1N1B4_510 0.60 -0.1 602,644 + Pf1N1B4_510 0.69 -0.1 602,644 + Pf1N1B4_510 0.69 -2.9 602,645 - Pf1N1B4_510 0.69 -0.1 602,685 + -1.1 602,685 + Pf1N1B4_510 0.72 -1.2 603,159 - Pf1N1B4_511 0.26 -1.7 603,212 + Pf1N1B4_511 0.67 +0.5 603,212 + Pf1N1B4_511 0.67 -1.1 603,213 - Pf1N1B4_511 0.67 -0.8 603,213 - Pf1N1B4_511 0.67 -2.9 603,223 + Pf1N1B4_511 0.75 +0.2 603,224 - Pf1N1B4_511 0.76 -0.8 603,224 - Pf1N1B4_511 0.76 +0.3 603,224 - Pf1N1B4_511 0.76 -2.9 603,224 - -1.7 603,325 + -2.4 603,325 + -0.1 603,325 + +0.9 603,325 + -1.4 603,325 + -0.3 603,325 + -1.1 603,325 + -1.4 603,433 + +0.5 603,433 + +0.7 603,433 + +0.8 603,433 + -1.4 603,433 + +0.7 603,527 - Pf1N1B4_512 0.22 -1.1 603,527 - Pf1N1B4_512 0.22 -0.1 603,652 + Pf1N1B4_512 0.38 -1.1 603,653 - Pf1N1B4_512 0.38 +1.5 603,653 - Pf1N1B4_512 0.38 -2.1 603,653 - Pf1N1B4_512 0.38 +0.1 603,746 - Pf1N1B4_512 0.51 +1.5 603,746 - Pf1N1B4_512 0.51 -2.1 603,862 + Pf1N1B4_512 0.66 +0.2 603,863 - -1.1 603,863 - Pf1N1B4_512 0.66 -1.1 603,878 - Pf1N1B4_512 0.68 +0.3 603,882 - Pf1N1B4_512 0.69 -1.1 603,882 - Pf1N1B4_512 0.69 -0.1 603,882 - Pf1N1B4_512 0.69 -1.4 603,882 - Pf1N1B4_512 0.69 -1.4 603,882 - Pf1N1B4_512 0.69 -0.1 603,882 - Pf1N1B4_512 0.69 +0.2 603,882 - Pf1N1B4_512 0.69 -0.1 603,882 - Pf1N1B4_512 0.69 -0.4
Or see this region's nucleotide sequence