Experiment: 2'-Deoxyinosine 5 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_05799 and H281DRAFT_05800 are separated by 147 nucleotides H281DRAFT_05800 and H281DRAFT_05801 are separated by 11 nucleotides H281DRAFT_05801 and H281DRAFT_05802 are separated by 317 nucleotides
H281DRAFT_05799: H281DRAFT_05799 - adenosylhomocysteinase, at 74,252 to 75,673
_05799
H281DRAFT_05800: H281DRAFT_05800 - putative membrane protein, at 75,821 to 76,174
_05800
H281DRAFT_05801: H281DRAFT_05801 - 5,10-methylenetetrahydrofolate reductase (NAD(P)), at 76,186 to 77,016
_05801
H281DRAFT_05802: H281DRAFT_05802 - dipeptide transport system substrate-binding protein, at 77,334 to 78,962
_05802
Position (kb) 76
77
78 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2 at 75.671 kb on + strand at 75.671 kb on + strand at 75.671 kb on + strand at 75.671 kb on + strand at 75.671 kb on + strand at 75.675 kb on + strand at 75.676 kb on - strand at 75.818 kb on + strand at 75.818 kb on + strand at 75.818 kb on + strand at 75.818 kb on + strand at 75.818 kb on + strand at 75.818 kb on + strand at 75.818 kb on + strand at 75.819 kb on - strand at 75.819 kb on - strand at 75.819 kb on - strand at 75.819 kb on - strand at 75.819 kb on - strand at 75.875 kb on + strand, within H281DRAFT_05800 at 75.875 kb on + strand, within H281DRAFT_05800 at 75.876 kb on - strand, within H281DRAFT_05800 at 75.876 kb on - strand, within H281DRAFT_05800 at 75.876 kb on - strand, within H281DRAFT_05800 at 75.930 kb on + strand, within H281DRAFT_05800 at 76.117 kb on + strand, within H281DRAFT_05800 at 76.117 kb on + strand, within H281DRAFT_05800 at 76.117 kb on + strand, within H281DRAFT_05800 at 76.117 kb on + strand, within H281DRAFT_05800 at 76.177 kb on + strand at 76.177 kb on + strand at 76.177 kb on + strand at 76.178 kb on - strand at 76.178 kb on - strand at 76.278 kb on - strand, within H281DRAFT_05801 at 76.465 kb on - strand, within H281DRAFT_05801 at 76.546 kb on - strand, within H281DRAFT_05801 at 76.600 kb on + strand, within H281DRAFT_05801 at 76.600 kb on + strand, within H281DRAFT_05801 at 76.600 kb on + strand, within H281DRAFT_05801 at 76.687 kb on + strand, within H281DRAFT_05801 at 76.708 kb on + strand, within H281DRAFT_05801 at 76.961 kb on - strand at 77.014 kb on + strand at 77.015 kb on - strand at 77.141 kb on + strand at 77.141 kb on + strand at 77.141 kb on + strand at 77.142 kb on - strand at 77.287 kb on - strand at 77.287 kb on - strand at 77.287 kb on - strand at 77.287 kb on - strand at 77.298 kb on - strand at 77.493 kb on + strand at 77.493 kb on + strand at 77.493 kb on + strand at 77.493 kb on + strand at 77.494 kb on - strand at 77.523 kb on - strand, within H281DRAFT_05802 at 77.535 kb on + strand, within H281DRAFT_05802 at 77.535 kb on + strand, within H281DRAFT_05802 at 77.535 kb on + strand, within H281DRAFT_05802 at 77.625 kb on + strand, within H281DRAFT_05802 at 77.625 kb on + strand, within H281DRAFT_05802 at 77.625 kb on + strand, within H281DRAFT_05802 at 77.625 kb on + strand, within H281DRAFT_05802 at 77.625 kb on + strand, within H281DRAFT_05802 at 77.626 kb on - strand, within H281DRAFT_05802 at 77.778 kb on + strand, within H281DRAFT_05802 at 77.836 kb on - strand, within H281DRAFT_05802 at 77.898 kb on + strand, within H281DRAFT_05802 at 77.922 kb on + strand, within H281DRAFT_05802 at 78.006 kb on + strand, within H281DRAFT_05802 at 78.006 kb on + strand, within H281DRAFT_05802 at 78.008 kb on + strand, within H281DRAFT_05802
Per-strain Table
Position Strand Gene LocusTag Fraction 2'-Deoxyinosine 5 mM (C) remove 75,671 + +0.2 75,671 + -0.2 75,671 + -0.0 75,671 + +0.8 75,671 + -1.7 75,675 + -0.2 75,676 - -3.5 75,818 + -1.0 75,818 + +1.2 75,818 + -0.5 75,818 + -0.3 75,818 + +0.8 75,818 + -0.1 75,818 + -2.9 75,819 - -4.2 75,819 - -1.6 75,819 - -3.5 75,819 - -2.9 75,819 - -2.9 75,875 + H281DRAFT_05800 0.15 -0.0 75,875 + H281DRAFT_05800 0.15 -1.6 75,876 - H281DRAFT_05800 0.16 -2.6 75,876 - H281DRAFT_05800 0.16 -4.8 75,876 - H281DRAFT_05800 0.16 -4.6 75,930 + H281DRAFT_05800 0.31 +1.8 76,117 + H281DRAFT_05800 0.84 -1.5 76,117 + H281DRAFT_05800 0.84 -1.6 76,117 + H281DRAFT_05800 0.84 -0.3 76,117 + H281DRAFT_05800 0.84 -0.4 76,177 + -0.2 76,177 + -0.2 76,177 + -1.0 76,178 - -1.6 76,178 - -1.0 76,278 - H281DRAFT_05801 0.11 -3.4 76,465 - H281DRAFT_05801 0.34 -3.1 76,546 - H281DRAFT_05801 0.43 -3.8 76,600 + H281DRAFT_05801 0.50 -4.0 76,600 + H281DRAFT_05801 0.50 -3.5 76,600 + H281DRAFT_05801 0.50 -3.1 76,687 + H281DRAFT_05801 0.60 -4.9 76,708 + H281DRAFT_05801 0.63 -3.7 76,961 - -4.7 77,014 + -0.4 77,015 - -0.4 77,141 + -0.9 77,141 + +0.5 77,141 + -0.3 77,142 - -0.0 77,287 - +0.8 77,287 - +0.6 77,287 - +0.3 77,287 - +0.9 77,298 - -0.5 77,493 + +0.2 77,493 + +1.1 77,493 + +0.3 77,493 + -1.6 77,494 - -0.0 77,523 - H281DRAFT_05802 0.12 +0.8 77,535 + H281DRAFT_05802 0.12 +0.4 77,535 + H281DRAFT_05802 0.12 +0.9 77,535 + H281DRAFT_05802 0.12 +0.1 77,625 + H281DRAFT_05802 0.18 +0.1 77,625 + H281DRAFT_05802 0.18 -0.0 77,625 + H281DRAFT_05802 0.18 +0.8 77,625 + H281DRAFT_05802 0.18 -1.3 77,625 + H281DRAFT_05802 0.18 -0.2 77,626 - H281DRAFT_05802 0.18 +0.4 77,778 + H281DRAFT_05802 0.27 +1.7 77,836 - H281DRAFT_05802 0.31 +0.2 77,898 + H281DRAFT_05802 0.35 +0.0 77,922 + H281DRAFT_05802 0.36 +1.2 78,006 + H281DRAFT_05802 0.41 +0.9 78,006 + H281DRAFT_05802 0.41 -0.3 78,008 + H281DRAFT_05802 0.41 -0.1
Or see this region's nucleotide sequence