Experiment: 2'-Deoxyinosine 5 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_02908 and H281DRAFT_02909 are separated by 494 nucleotides H281DRAFT_02909 and H281DRAFT_02910 are separated by 125 nucleotides H281DRAFT_02910 and H281DRAFT_02911 are separated by 394 nucleotides H281DRAFT_02911 and H281DRAFT_02912 are separated by 128 nucleotides
H281DRAFT_02908: H281DRAFT_02908 - RNA polymerase, sigma 54 subunit, RpoN/SigL, at 516,665 to 518,146
_02908
H281DRAFT_02909: H281DRAFT_02909 - Predicted periplasmic or secreted lipoprotein, at 518,641 to 518,979
_02909
H281DRAFT_02910: H281DRAFT_02910 - conserved hypothetical protein, at 519,105 to 519,554
_02910
H281DRAFT_02911: H281DRAFT_02911 - hypothetical protein, at 519,949 to 520,203
_02911
H281DRAFT_02912: H281DRAFT_02912 - Glycine zipper, at 520,332 to 520,913
_02912
Position (kb)
519
520 Strain fitness (log2 ratio)
-1
0
1 at 518.835 kb on + strand, within H281DRAFT_02909 at 518.835 kb on + strand, within H281DRAFT_02909 at 519.107 kb on - strand at 519.121 kb on + strand at 519.232 kb on + strand, within H281DRAFT_02910 at 519.233 kb on - strand, within H281DRAFT_02910 at 519.559 kb on + strand at 519.560 kb on - strand at 519.577 kb on - strand at 519.761 kb on + strand at 519.761 kb on + strand at 519.762 kb on - strand at 520.351 kb on + strand at 520.352 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 2'-Deoxyinosine 5 mM (C) remove 518,835 + H281DRAFT_02909 0.57 +0.6 518,835 + H281DRAFT_02909 0.57 +1.0 519,107 - +0.2 519,121 + +0.2 519,232 + H281DRAFT_02910 0.28 -0.3 519,233 - H281DRAFT_02910 0.28 -1.6 519,559 + +0.8 519,560 - +0.1 519,577 - -0.4 519,761 + +0.5 519,761 + -0.4 519,762 - +0.5 520,351 + +0.7 520,352 - +0.2
Or see this region's nucleotide sequence