Experiment: 2'-Deoxyinosine 5 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_01050 and H281DRAFT_01051 overlap by 4 nucleotides H281DRAFT_01051 and H281DRAFT_01052 are separated by 27 nucleotides H281DRAFT_01052 and H281DRAFT_01053 are separated by 34 nucleotides H281DRAFT_01053 and H281DRAFT_01054 are separated by 17 nucleotides
H281DRAFT_01050: H281DRAFT_01050 - maleylacetate reductase, at 1,180,503 to 1,181,597
_01050
H281DRAFT_01051: H281DRAFT_01051 - hydroxyquinol 1,2-dioxygenase, at 1,181,594 to 1,182,454
_01051
H281DRAFT_01052: H281DRAFT_01052 - hypothetical protein, at 1,182,482 to 1,182,766
_01052
H281DRAFT_01053: H281DRAFT_01053 - 3-hydroxyisobutyrate dehydrogenase, at 1,182,801 to 1,183,706
_01053
H281DRAFT_01054: H281DRAFT_01054 - (R,R)-butanediol dehydrogenase / meso-butanediol dehydrogenase / diacetyl reductase, at 1,183,724 to 1,184,794
_01054
Position (kb) 1182
1183 Strain fitness (log2 ratio) -1
0
1 at 1182.706 kb on - strand, within H281DRAFT_01052 at 1182.760 kb on - strand at 1182.760 kb on - strand at 1182.766 kb on + strand at 1182.881 kb on - strand at 1183.412 kb on - strand, within H281DRAFT_01053 at 1183.594 kb on + strand, within H281DRAFT_01053
Per-strain Table
Position Strand Gene LocusTag Fraction 2'-Deoxyinosine 5 mM (C) remove 1,182,706 - H281DRAFT_01052 0.79 -0.9 1,182,760 - +0.1 1,182,760 - -0.1 1,182,766 + -0.7 1,182,881 - +0.5 1,183,412 - H281DRAFT_01053 0.67 -0.7 1,183,594 + H281DRAFT_01053 0.88 +0.4
Or see this region's nucleotide sequence