Experiment: 2'-Deoxyinosine 5 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_00117 and H281DRAFT_00118 are separated by 20 nucleotides H281DRAFT_00118 and H281DRAFT_00119 are separated by 52 nucleotides H281DRAFT_00119 and H281DRAFT_00120 are separated by 82 nucleotides
H281DRAFT_00117: H281DRAFT_00117 - sulfate adenylyltransferase subunit 2, at 124,683 to 125,645
_00117
H281DRAFT_00118: H281DRAFT_00118 - sulfate adenylyltransferase subunit 1, at 125,666 to 126,979
_00118
H281DRAFT_00119: H281DRAFT_00119 - uroporphyrinogen-III C-methyltransferase, at 127,032 to 127,787
_00119
H281DRAFT_00120: H281DRAFT_00120 - sirohydrochlorin cobaltochelatase, at 127,870 to 128,250
_00120
Position (kb) 125
126
127 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 124.944 kb on + strand, within H281DRAFT_00117 at 125.247 kb on + strand, within H281DRAFT_00117 at 125.248 kb on - strand, within H281DRAFT_00117 at 125.248 kb on - strand, within H281DRAFT_00117 at 125.248 kb on - strand, within H281DRAFT_00117 at 125.326 kb on - strand, within H281DRAFT_00117 at 125.362 kb on - strand, within H281DRAFT_00117 at 125.362 kb on - strand, within H281DRAFT_00117 at 125.624 kb on + strand at 125.625 kb on - strand at 125.672 kb on - strand at 125.763 kb on - strand at 125.893 kb on + strand, within H281DRAFT_00118 at 125.893 kb on + strand, within H281DRAFT_00118 at 125.909 kb on + strand, within H281DRAFT_00118 at 125.915 kb on + strand, within H281DRAFT_00118 at 125.972 kb on + strand at 126.162 kb on - strand, within H281DRAFT_00118 at 126.197 kb on + strand, within H281DRAFT_00118 at 126.197 kb on + strand, within H281DRAFT_00118 at 126.622 kb on + strand, within H281DRAFT_00118 at 126.790 kb on + strand, within H281DRAFT_00118 at 126.791 kb on - strand, within H281DRAFT_00118 at 126.791 kb on - strand, within H281DRAFT_00118 at 126.884 kb on + strand at 126.884 kb on + strand at 127.037 kb on + strand at 127.038 kb on - strand at 127.082 kb on + strand at 127.162 kb on - strand, within H281DRAFT_00119 at 127.360 kb on - strand, within H281DRAFT_00119 at 127.524 kb on + strand, within H281DRAFT_00119 at 127.525 kb on - strand, within H281DRAFT_00119
Per-strain Table
Position Strand Gene LocusTag Fraction 2'-Deoxyinosine 5 mM (C) remove 124,944 + H281DRAFT_00117 0.27 -3.8 125,247 + H281DRAFT_00117 0.59 -3.2 125,248 - H281DRAFT_00117 0.59 -3.4 125,248 - H281DRAFT_00117 0.59 -3.0 125,248 - H281DRAFT_00117 0.59 -4.2 125,326 - H281DRAFT_00117 0.67 -2.2 125,362 - H281DRAFT_00117 0.71 -5.0 125,362 - H281DRAFT_00117 0.71 -3.1 125,624 + -4.4 125,625 - -5.1 125,672 - -2.1 125,763 - -2.3 125,893 + H281DRAFT_00118 0.17 -2.9 125,893 + H281DRAFT_00118 0.17 -2.3 125,909 + H281DRAFT_00118 0.18 -1.5 125,915 + H281DRAFT_00118 0.19 -0.8 125,972 + +1.4 126,162 - H281DRAFT_00118 0.38 -2.5 126,197 + H281DRAFT_00118 0.40 -2.5 126,197 + H281DRAFT_00118 0.40 -1.5 126,622 + H281DRAFT_00118 0.73 -1.2 126,790 + H281DRAFT_00118 0.86 -3.1 126,791 - H281DRAFT_00118 0.86 -2.6 126,791 - H281DRAFT_00118 0.86 -4.5 126,884 + -2.5 126,884 + -2.3 127,037 + +0.1 127,038 - -0.5 127,082 + -0.7 127,162 - H281DRAFT_00119 0.17 +1.0 127,360 - H281DRAFT_00119 0.43 -0.3 127,524 + H281DRAFT_00119 0.65 -0.2 127,525 - H281DRAFT_00119 0.65 -1.3
Or see this region's nucleotide sequence