Experiment: 2'-Deoxyinosine 5 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_05543 and H281DRAFT_05544 are separated by 250 nucleotides H281DRAFT_05544 and H281DRAFT_05545 are separated by 7 nucleotides H281DRAFT_05545 and H281DRAFT_05546 are separated by 312 nucleotides
H281DRAFT_05543: H281DRAFT_05543 - porin, GBP family, at 130,495 to 131,649
_05543
H281DRAFT_05544: H281DRAFT_05544 - NitT/TauT family transport system permease protein, at 131,900 to 132,775
_05544
H281DRAFT_05545: H281DRAFT_05545 - NitT/TauT family transport system ATP-binding protein, at 132,783 to 133,379
_05545
H281DRAFT_05546: H281DRAFT_05546 - NitT/TauT family transport system substrate-binding protein, at 133,692 to 134,699
_05546
Position (kb)
131
132
133 Strain fitness (log2 ratio)
-1
0
1
2 at 130.965 kb on - strand at 131.097 kb on + strand, within H281DRAFT_05543 at 131.156 kb on + strand, within H281DRAFT_05543 at 131.157 kb on - strand, within H281DRAFT_05543 at 131.327 kb on + strand, within H281DRAFT_05543 at 131.449 kb on + strand, within H281DRAFT_05543 at 131.491 kb on - strand, within H281DRAFT_05543 at 131.988 kb on - strand, within H281DRAFT_05544 at 132.550 kb on - strand, within H281DRAFT_05544 at 132.776 kb on - strand at 132.776 kb on - strand at 132.776 kb on - strand at 132.922 kb on + strand, within H281DRAFT_05545 at 133.464 kb on + strand at 133.464 kb on + strand at 133.465 kb on - strand at 133.554 kb on + strand at 133.607 kb on + strand at 133.607 kb on + strand at 133.607 kb on + strand at 133.625 kb on + strand at 133.630 kb on + strand at 133.633 kb on + strand at 133.673 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 2'-Deoxyinosine 5 mM (C) remove 130,965 - +0.9 131,097 + H281DRAFT_05543 0.52 +0.3 131,156 + H281DRAFT_05543 0.57 +0.9 131,157 - H281DRAFT_05543 0.57 +0.2 131,327 + H281DRAFT_05543 0.72 -0.1 131,449 + H281DRAFT_05543 0.83 -0.9 131,491 - H281DRAFT_05543 0.86 +0.5 131,988 - H281DRAFT_05544 0.10 -1.1 132,550 - H281DRAFT_05544 0.74 -1.7 132,776 - -1.4 132,776 - -0.4 132,776 - +0.4 132,922 + H281DRAFT_05545 0.23 +0.4 133,464 + +0.3 133,464 + -0.3 133,465 - -1.0 133,554 + -0.7 133,607 + +0.2 133,607 + +0.7 133,607 + -0.3 133,625 + +1.3 133,630 + +0.4 133,633 + +1.9 133,673 + +0.1
Or see this region's nucleotide sequence