Experiment: A22 0.02 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_1552 and Shewana3_1553 are separated by 87 nucleotides Shewana3_1553 and pflA are separated by 15 nucleotides pflA and Shewana3_1555 are separated by 63 nucleotides
Shewana3_1552: Shewana3_1552 - acetate kinase (RefSeq), at 1,808,697 to 1,809,896
_1552
Shewana3_1553: Shewana3_1553 - hypothetical protein (RefSeq), at 1,809,984 to 1,810,481
_1553
Shewana3_1554: pflA - pyruvate formate lyase-activating enzyme 1 (RefSeq), at 1,810,497 to 1,811,237
pflA
Shewana3_1555: Shewana3_1555 - formate acetyltransferase (RefSeq), at 1,811,301 to 1,813,583
_1555
Position (kb)
1809
1810
1811 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1809.022 kb on + strand, within Shewana3_1552 at 1809.193 kb on - strand, within Shewana3_1552 at 1809.365 kb on - strand, within Shewana3_1552 at 1809.451 kb on + strand, within Shewana3_1552 at 1810.002 kb on - strand at 1810.009 kb on - strand at 1810.011 kb on + strand at 1810.213 kb on - strand, within Shewana3_1553 at 1810.266 kb on + strand, within Shewana3_1553 at 1810.475 kb on + strand at 1810.568 kb on - strand at 1810.797 kb on - strand, within pflA at 1810.865 kb on - strand, within pflA at 1810.965 kb on - strand, within pflA at 1811.141 kb on + strand, within pflA at 1811.156 kb on + strand, within pflA at 1811.318 kb on + strand at 1811.374 kb on + strand at 1811.382 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction A22 0.02 mg/ml remove 1,809,022 + Shewana3_1552 0.27 +0.6 1,809,193 - Shewana3_1552 0.41 -0.1 1,809,365 - Shewana3_1552 0.56 +0.1 1,809,451 + Shewana3_1552 0.63 +0.6 1,810,002 - +0.6 1,810,009 - -2.2 1,810,011 + +0.6 1,810,213 - Shewana3_1553 0.46 -1.2 1,810,266 + Shewana3_1553 0.57 +0.6 1,810,475 + -1.0 1,810,568 - +0.6 1,810,797 - pflA Shewana3_1554 0.40 +2.1 1,810,865 - pflA Shewana3_1554 0.50 +0.6 1,810,965 - pflA Shewana3_1554 0.63 +0.6 1,811,141 + pflA Shewana3_1554 0.87 -2.0 1,811,156 + pflA Shewana3_1554 0.89 +0.8 1,811,318 + -0.4 1,811,374 + +0.6 1,811,382 - +1.4
Or see this region's nucleotide sequence