Experiment: L-Histidine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1589 and Pf1N1B4_1590 are separated by 23 nucleotides Pf1N1B4_1590 and Pf1N1B4_1591 are separated by 107 nucleotides Pf1N1B4_1591 and Pf1N1B4_1592 are separated by 118 nucleotides Pf1N1B4_1592 and Pf1N1B4_1593 are separated by 33 nucleotides
Pf1N1B4_1589 - Conserved hypothetical protein (perhaps related to histidine degradation), at 1,830,817 to 1,831,386
_1589
Pf1N1B4_1590 - Histidine utilization repressor, at 1,831,410 to 1,832,171
_1590
Pf1N1B4_1591 - Formiminoglutamic iminohydrolase (EC 3.5.3.13) (from data) , at 1,832,279 to 1,833,643
_1591
Pf1N1B4_1592 - Outer membrane lipoprotein Blc, at 1,833,762 to 1,834,331
_1592
Pf1N1B4_1593 - Phosphonate ABC transporter phosphate-binding periplasmic component (TC 3.A.1.9.1), at 1,834,365 to 1,834,619
_1593
Position (kb)
1832
1833
1834 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3 at 1831.345 kb on - strand at 1831.397 kb on + strand at 1831.397 kb on + strand at 1831.397 kb on + strand at 1831.397 kb on + strand at 1831.398 kb on - strand at 1831.398 kb on - strand at 1831.401 kb on + strand at 1831.401 kb on + strand at 1831.402 kb on - strand at 1831.402 kb on - strand at 1831.402 kb on - strand at 1831.411 kb on + strand at 1831.411 kb on + strand at 1831.411 kb on + strand at 1831.411 kb on + strand at 1831.411 kb on + strand at 1831.411 kb on + strand at 1831.412 kb on - strand at 1831.412 kb on - strand at 1831.412 kb on - strand at 1831.621 kb on + strand, within Pf1N1B4_1590 at 1831.621 kb on + strand, within Pf1N1B4_1590 at 1831.621 kb on + strand, within Pf1N1B4_1590 at 1831.622 kb on - strand, within Pf1N1B4_1590 at 1831.622 kb on - strand, within Pf1N1B4_1590 at 1831.622 kb on - strand, within Pf1N1B4_1590 at 1831.622 kb on - strand, within Pf1N1B4_1590 at 1831.627 kb on + strand, within Pf1N1B4_1590 at 1831.627 kb on + strand, within Pf1N1B4_1590 at 1831.627 kb on + strand, within Pf1N1B4_1590 at 1831.627 kb on + strand, within Pf1N1B4_1590 at 1831.628 kb on - strand, within Pf1N1B4_1590 at 1831.628 kb on - strand, within Pf1N1B4_1590 at 1831.660 kb on + strand, within Pf1N1B4_1590 at 1831.660 kb on + strand, within Pf1N1B4_1590 at 1831.660 kb on + strand, within Pf1N1B4_1590 at 1831.661 kb on - strand, within Pf1N1B4_1590 at 1831.661 kb on - strand, within Pf1N1B4_1590 at 1831.713 kb on - strand, within Pf1N1B4_1590 at 1831.916 kb on + strand, within Pf1N1B4_1590 at 1831.916 kb on + strand, within Pf1N1B4_1590 at 1831.916 kb on + strand, within Pf1N1B4_1590 at 1831.917 kb on - strand, within Pf1N1B4_1590 at 1832.095 kb on + strand at 1832.095 kb on + strand at 1832.095 kb on + strand at 1832.095 kb on + strand at 1832.095 kb on + strand at 1832.096 kb on - strand at 1832.096 kb on - strand at 1832.096 kb on - strand at 1832.096 kb on - strand at 1832.203 kb on + strand at 1832.203 kb on + strand at 1832.203 kb on + strand at 1832.203 kb on + strand at 1832.203 kb on + strand at 1832.204 kb on - strand at 1832.239 kb on + strand at 1832.239 kb on + strand at 1832.239 kb on + strand at 1832.239 kb on + strand at 1832.240 kb on - strand at 1832.240 kb on - strand at 1832.240 kb on - strand at 1832.240 kb on - strand at 1832.240 kb on - strand at 1832.240 kb on - strand at 1832.241 kb on + strand at 1832.241 kb on + strand at 1832.242 kb on - strand at 1832.242 kb on - strand at 1832.245 kb on + strand at 1832.245 kb on + strand at 1832.245 kb on + strand at 1832.245 kb on + strand at 1832.245 kb on + strand at 1832.246 kb on - strand at 1832.246 kb on - strand at 1832.246 kb on - strand at 1832.246 kb on - strand at 1832.246 kb on - strand at 1832.246 kb on - strand at 1832.246 kb on - strand at 1832.248 kb on - strand at 1832.248 kb on - strand at 1832.314 kb on + strand at 1832.334 kb on + strand at 1832.334 kb on + strand at 1832.334 kb on + strand at 1832.335 kb on - strand at 1832.335 kb on - strand at 1832.335 kb on - strand at 1832.335 kb on - strand at 1832.335 kb on - strand at 1832.591 kb on + strand, within Pf1N1B4_1591 at 1832.592 kb on - strand, within Pf1N1B4_1591 at 1832.592 kb on - strand, within Pf1N1B4_1591 at 1832.592 kb on - strand, within Pf1N1B4_1591 at 1832.592 kb on - strand, within Pf1N1B4_1591 at 1832.592 kb on - strand, within Pf1N1B4_1591 at 1832.639 kb on + strand, within Pf1N1B4_1591 at 1832.639 kb on + strand, within Pf1N1B4_1591 at 1832.639 kb on + strand, within Pf1N1B4_1591 at 1832.639 kb on + strand, within Pf1N1B4_1591 at 1832.640 kb on - strand, within Pf1N1B4_1591 at 1832.640 kb on - strand, within Pf1N1B4_1591 at 1832.640 kb on - strand, within Pf1N1B4_1591 at 1832.640 kb on - strand, within Pf1N1B4_1591 at 1832.643 kb on + strand, within Pf1N1B4_1591 at 1832.643 kb on + strand, within Pf1N1B4_1591 at 1832.643 kb on + strand, within Pf1N1B4_1591 at 1832.643 kb on + strand, within Pf1N1B4_1591 at 1832.643 kb on + strand, within Pf1N1B4_1591 at 1832.643 kb on + strand, within Pf1N1B4_1591 at 1832.644 kb on - strand, within Pf1N1B4_1591 at 1832.644 kb on - strand, within Pf1N1B4_1591 at 1832.644 kb on - strand, within Pf1N1B4_1591 at 1832.753 kb on + strand, within Pf1N1B4_1591 at 1832.753 kb on + strand, within Pf1N1B4_1591 at 1832.753 kb on + strand, within Pf1N1B4_1591 at 1832.754 kb on - strand, within Pf1N1B4_1591 at 1832.754 kb on - strand, within Pf1N1B4_1591 at 1832.754 kb on - strand, within Pf1N1B4_1591 at 1832.807 kb on - strand, within Pf1N1B4_1591 at 1832.825 kb on + strand, within Pf1N1B4_1591 at 1832.826 kb on - strand, within Pf1N1B4_1591 at 1832.826 kb on - strand, within Pf1N1B4_1591 at 1833.047 kb on + strand, within Pf1N1B4_1591 at 1833.048 kb on - strand, within Pf1N1B4_1591 at 1833.150 kb on + strand, within Pf1N1B4_1591 at 1833.150 kb on + strand, within Pf1N1B4_1591 at 1833.150 kb on + strand, within Pf1N1B4_1591 at 1833.150 kb on + strand, within Pf1N1B4_1591 at 1833.151 kb on - strand, within Pf1N1B4_1591 at 1833.151 kb on - strand, within Pf1N1B4_1591 at 1833.151 kb on - strand, within Pf1N1B4_1591 at 1833.151 kb on - strand, within Pf1N1B4_1591 at 1833.326 kb on + strand, within Pf1N1B4_1591 at 1833.326 kb on + strand, within Pf1N1B4_1591 at 1833.327 kb on - strand, within Pf1N1B4_1591 at 1833.327 kb on - strand, within Pf1N1B4_1591 at 1833.327 kb on - strand, within Pf1N1B4_1591 at 1833.327 kb on - strand, within Pf1N1B4_1591 at 1833.327 kb on - strand, within Pf1N1B4_1591 at 1833.327 kb on - strand, within Pf1N1B4_1591 at 1833.362 kb on + strand, within Pf1N1B4_1591 at 1833.362 kb on + strand, within Pf1N1B4_1591 at 1833.362 kb on + strand, within Pf1N1B4_1591 at 1833.362 kb on + strand, within Pf1N1B4_1591 at 1833.363 kb on - strand, within Pf1N1B4_1591 at 1833.467 kb on + strand, within Pf1N1B4_1591 at 1833.467 kb on + strand, within Pf1N1B4_1591 at 1833.601 kb on + strand at 1833.602 kb on - strand at 1833.745 kb on + strand at 1833.745 kb on + strand at 1833.745 kb on + strand at 1833.745 kb on + strand at 1833.745 kb on + strand at 1833.746 kb on - strand at 1833.746 kb on - strand at 1833.746 kb on - strand at 1833.746 kb on - strand at 1833.746 kb on - strand at 1833.746 kb on - strand at 1833.746 kb on - strand at 1833.746 kb on - strand at 1833.763 kb on + strand at 1833.763 kb on + strand at 1833.763 kb on + strand at 1833.764 kb on - strand at 1833.764 kb on - strand at 1833.764 kb on - strand at 1833.764 kb on - strand at 1833.789 kb on - strand at 1833.940 kb on + strand, within Pf1N1B4_1592 at 1833.940 kb on + strand, within Pf1N1B4_1592 at 1833.940 kb on + strand, within Pf1N1B4_1592 at 1833.940 kb on + strand, within Pf1N1B4_1592 at 1833.941 kb on - strand, within Pf1N1B4_1592 at 1833.941 kb on - strand, within Pf1N1B4_1592 at 1833.941 kb on - strand, within Pf1N1B4_1592 at 1833.941 kb on - strand, within Pf1N1B4_1592 at 1833.955 kb on + strand, within Pf1N1B4_1592 at 1833.955 kb on + strand, within Pf1N1B4_1592 at 1833.955 kb on + strand, within Pf1N1B4_1592 at 1833.956 kb on - strand, within Pf1N1B4_1592 at 1833.956 kb on - strand, within Pf1N1B4_1592 at 1833.956 kb on - strand, within Pf1N1B4_1592 at 1833.956 kb on - strand, within Pf1N1B4_1592 at 1833.967 kb on + strand, within Pf1N1B4_1592 at 1833.968 kb on - strand, within Pf1N1B4_1592 at 1833.968 kb on - strand, within Pf1N1B4_1592 at 1833.968 kb on - strand, within Pf1N1B4_1592 at 1834.057 kb on + strand at 1834.144 kb on + strand, within Pf1N1B4_1592 at 1834.145 kb on - strand, within Pf1N1B4_1592 at 1834.145 kb on - strand, within Pf1N1B4_1592 at 1834.180 kb on + strand, within Pf1N1B4_1592 at 1834.180 kb on + strand, within Pf1N1B4_1592 at 1834.180 kb on + strand, within Pf1N1B4_1592 at 1834.181 kb on - strand, within Pf1N1B4_1592 at 1834.266 kb on + strand, within Pf1N1B4_1592 at 1834.267 kb on - strand, within Pf1N1B4_1592 at 1834.267 kb on - strand, within Pf1N1B4_1592 at 1834.267 kb on - strand, within Pf1N1B4_1592 at 1834.312 kb on - strand at 1834.333 kb on - strand at 1834.355 kb on + strand at 1834.411 kb on + strand, within Pf1N1B4_1593 at 1834.411 kb on + strand, within Pf1N1B4_1593 at 1834.411 kb on + strand, within Pf1N1B4_1593 at 1834.412 kb on - strand, within Pf1N1B4_1593 at 1834.425 kb on - strand, within Pf1N1B4_1593 at 1834.604 kb on + strand at 1834.604 kb on + strand at 1834.624 kb on + strand at 1834.624 kb on + strand at 1834.624 kb on + strand at 1834.625 kb on - strand at 1834.625 kb on - strand at 1834.625 kb on - strand at 1834.625 kb on - strand at 1834.625 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Histidine (C) remove 1,831,345 - -0.9 1,831,397 + -3.6 1,831,397 + -1.5 1,831,397 + +0.1 1,831,397 + -2.3 1,831,398 - +0.1 1,831,398 - -0.3 1,831,401 + -0.3 1,831,401 + -2.5 1,831,402 - -1.5 1,831,402 - +0.4 1,831,402 - -0.2 1,831,411 + -2.9 1,831,411 + -3.1 1,831,411 + -0.9 1,831,411 + +0.1 1,831,411 + -2.9 1,831,411 + -1.5 1,831,412 - +0.1 1,831,412 - +0.7 1,831,412 - +0.1 1,831,621 + Pf1N1B4_1590 0.28 -0.3 1,831,621 + Pf1N1B4_1590 0.28 -0.7 1,831,621 + Pf1N1B4_1590 0.28 -0.7 1,831,622 - Pf1N1B4_1590 0.28 +2.5 1,831,622 - Pf1N1B4_1590 0.28 +2.2 1,831,622 - Pf1N1B4_1590 0.28 +3.4 1,831,622 - Pf1N1B4_1590 0.28 +1.7 1,831,627 + Pf1N1B4_1590 0.28 -0.5 1,831,627 + Pf1N1B4_1590 0.28 -1.7 1,831,627 + Pf1N1B4_1590 0.28 +0.7 1,831,627 + Pf1N1B4_1590 0.28 -1.7 1,831,628 - Pf1N1B4_1590 0.29 +2.9 1,831,628 - Pf1N1B4_1590 0.29 +1.7 1,831,660 + Pf1N1B4_1590 0.33 -1.5 1,831,660 + Pf1N1B4_1590 0.33 -1.1 1,831,660 + Pf1N1B4_1590 0.33 -0.9 1,831,661 - Pf1N1B4_1590 0.33 +2.5 1,831,661 - Pf1N1B4_1590 0.33 +2.7 1,831,713 - Pf1N1B4_1590 0.40 +3.0 1,831,916 + Pf1N1B4_1590 0.66 -2.3 1,831,916 + Pf1N1B4_1590 0.66 +0.1 1,831,916 + Pf1N1B4_1590 0.66 +0.1 1,831,917 - Pf1N1B4_1590 0.67 +2.3 1,832,095 + -1.5 1,832,095 + -1.7 1,832,095 + +0.1 1,832,095 + -1.3 1,832,095 + -2.5 1,832,096 - +0.2 1,832,096 - -0.7 1,832,096 - -1.5 1,832,096 - +0.5 1,832,203 + -0.9 1,832,203 + -1.7 1,832,203 + +1.1 1,832,203 + -1.2 1,832,203 + -0.7 1,832,204 - -0.9 1,832,239 + -4.2 1,832,239 + -1.5 1,832,239 + -2.1 1,832,239 + -1.7 1,832,240 - -1.5 1,832,240 - -2.7 1,832,240 - -0.9 1,832,240 - -1.7 1,832,240 - +0.1 1,832,240 - -2.1 1,832,241 + -2.5 1,832,241 + -0.5 1,832,242 - -0.9 1,832,242 - -0.9 1,832,245 + -3.6 1,832,245 + -0.5 1,832,245 + -0.5 1,832,245 + -5.1 1,832,245 + -1.9 1,832,246 - -2.3 1,832,246 - -1.5 1,832,246 - -1.5 1,832,246 - -3.6 1,832,246 - -0.3 1,832,246 - -2.1 1,832,246 - -1.2 1,832,248 - -3.1 1,832,248 - +0.1 1,832,314 + -3.8 1,832,334 + -1.5 1,832,334 + -2.5 1,832,334 + -1.5 1,832,335 - -1.5 1,832,335 - -1.7 1,832,335 - -2.5 1,832,335 - -2.7 1,832,335 - -2.5 1,832,591 + Pf1N1B4_1591 0.23 -1.5 1,832,592 - Pf1N1B4_1591 0.23 -2.9 1,832,592 - Pf1N1B4_1591 0.23 -4.5 1,832,592 - Pf1N1B4_1591 0.23 -3.7 1,832,592 - Pf1N1B4_1591 0.23 +0.1 1,832,592 - Pf1N1B4_1591 0.23 -1.9 1,832,639 + Pf1N1B4_1591 0.26 -1.5 1,832,639 + Pf1N1B4_1591 0.26 -2.9 1,832,639 + Pf1N1B4_1591 0.26 -1.9 1,832,639 + Pf1N1B4_1591 0.26 -0.8 1,832,640 - Pf1N1B4_1591 0.26 -1.9 1,832,640 - Pf1N1B4_1591 0.26 -3.1 1,832,640 - Pf1N1B4_1591 0.26 -3.8 1,832,640 - Pf1N1B4_1591 0.26 -0.9 1,832,643 + Pf1N1B4_1591 0.27 -3.1 1,832,643 + Pf1N1B4_1591 0.27 -2.7 1,832,643 + Pf1N1B4_1591 0.27 -0.9 1,832,643 + Pf1N1B4_1591 0.27 -3.4 1,832,643 + Pf1N1B4_1591 0.27 -0.9 1,832,643 + Pf1N1B4_1591 0.27 -1.5 1,832,644 - Pf1N1B4_1591 0.27 -0.9 1,832,644 - Pf1N1B4_1591 0.27 -0.9 1,832,644 - Pf1N1B4_1591 0.27 +0.1 1,832,753 + Pf1N1B4_1591 0.35 -3.1 1,832,753 + Pf1N1B4_1591 0.35 -3.3 1,832,753 + Pf1N1B4_1591 0.35 -1.9 1,832,754 - Pf1N1B4_1591 0.35 +0.1 1,832,754 - Pf1N1B4_1591 0.35 +0.1 1,832,754 - Pf1N1B4_1591 0.35 -0.9 1,832,807 - Pf1N1B4_1591 0.39 -2.7 1,832,825 + Pf1N1B4_1591 0.40 -0.7 1,832,826 - Pf1N1B4_1591 0.40 -2.3 1,832,826 - Pf1N1B4_1591 0.40 -2.6 1,833,047 + Pf1N1B4_1591 0.56 -0.9 1,833,048 - Pf1N1B4_1591 0.56 -3.1 1,833,150 + Pf1N1B4_1591 0.64 -3.1 1,833,150 + Pf1N1B4_1591 0.64 -1.9 1,833,150 + Pf1N1B4_1591 0.64 -2.5 1,833,150 + Pf1N1B4_1591 0.64 +1.1 1,833,151 - Pf1N1B4_1591 0.64 -1.9 1,833,151 - Pf1N1B4_1591 0.64 +0.1 1,833,151 - Pf1N1B4_1591 0.64 -1.9 1,833,151 - Pf1N1B4_1591 0.64 -2.5 1,833,326 + Pf1N1B4_1591 0.77 +1.1 1,833,326 + Pf1N1B4_1591 0.77 -1.5 1,833,327 - Pf1N1B4_1591 0.77 -1.9 1,833,327 - Pf1N1B4_1591 0.77 -0.9 1,833,327 - Pf1N1B4_1591 0.77 +1.1 1,833,327 - Pf1N1B4_1591 0.77 -0.9 1,833,327 - Pf1N1B4_1591 0.77 -0.9 1,833,327 - Pf1N1B4_1591 0.77 -2.3 1,833,362 + Pf1N1B4_1591 0.79 -1.5 1,833,362 + Pf1N1B4_1591 0.79 -2.3 1,833,362 + Pf1N1B4_1591 0.79 -0.9 1,833,362 + Pf1N1B4_1591 0.79 -2.3 1,833,363 - Pf1N1B4_1591 0.79 -2.3 1,833,467 + Pf1N1B4_1591 0.87 -2.3 1,833,467 + Pf1N1B4_1591 0.87 -0.5 1,833,601 + -2.9 1,833,602 - +0.1 1,833,745 + +0.5 1,833,745 + +0.5 1,833,745 + -0.5 1,833,745 + -0.2 1,833,745 + -0.5 1,833,746 - +2.1 1,833,746 - +0.1 1,833,746 - +0.1 1,833,746 - +1.1 1,833,746 - +0.7 1,833,746 - -0.3 1,833,746 - -0.9 1,833,746 - +1.1 1,833,763 + -1.0 1,833,763 + +0.1 1,833,763 + -0.9 1,833,764 - +0.1 1,833,764 - -0.9 1,833,764 - +1.1 1,833,764 - +1.1 1,833,789 - -0.8 1,833,940 + Pf1N1B4_1592 0.31 +0.4 1,833,940 + Pf1N1B4_1592 0.31 -0.9 1,833,940 + Pf1N1B4_1592 0.31 -1.5 1,833,940 + Pf1N1B4_1592 0.31 +0.4 1,833,941 - Pf1N1B4_1592 0.31 +0.9 1,833,941 - Pf1N1B4_1592 0.31 +0.1 1,833,941 - Pf1N1B4_1592 0.31 +0.9 1,833,941 - Pf1N1B4_1592 0.31 +0.1 1,833,955 + Pf1N1B4_1592 0.34 +0.1 1,833,955 + Pf1N1B4_1592 0.34 -0.7 1,833,955 + Pf1N1B4_1592 0.34 +1.2 1,833,956 - Pf1N1B4_1592 0.34 +0.9 1,833,956 - Pf1N1B4_1592 0.34 -0.1 1,833,956 - Pf1N1B4_1592 0.34 -0.5 1,833,956 - Pf1N1B4_1592 0.34 +0.1 1,833,967 + Pf1N1B4_1592 0.36 -0.3 1,833,968 - Pf1N1B4_1592 0.36 +1.6 1,833,968 - Pf1N1B4_1592 0.36 +0.1 1,833,968 - Pf1N1B4_1592 0.36 -0.3 1,834,057 + +1.7 1,834,144 + Pf1N1B4_1592 0.67 +0.1 1,834,145 - Pf1N1B4_1592 0.67 +0.1 1,834,145 - Pf1N1B4_1592 0.67 -1.5 1,834,180 + Pf1N1B4_1592 0.73 -1.6 1,834,180 + Pf1N1B4_1592 0.73 -1.3 1,834,180 + Pf1N1B4_1592 0.73 +0.4 1,834,181 - Pf1N1B4_1592 0.74 -0.3 1,834,266 + Pf1N1B4_1592 0.88 +0.7 1,834,267 - Pf1N1B4_1592 0.89 -1.1 1,834,267 - Pf1N1B4_1592 0.89 +1.3 1,834,267 - Pf1N1B4_1592 0.89 +0.1 1,834,312 - +0.1 1,834,333 - +0.7 1,834,355 + -1.2 1,834,411 + Pf1N1B4_1593 0.18 +0.1 1,834,411 + Pf1N1B4_1593 0.18 -1.9 1,834,411 + Pf1N1B4_1593 0.18 -0.7 1,834,412 - Pf1N1B4_1593 0.18 +0.2 1,834,425 - Pf1N1B4_1593 0.24 +0.1 1,834,604 + +0.3 1,834,604 + -1.3 1,834,624 + -2.1 1,834,624 + -1.5 1,834,624 + -1.5 1,834,625 - +1.8 1,834,625 - -0.7 1,834,625 - +0.7 1,834,625 - -0.5 1,834,625 - +1.1
Or see this region's nucleotide sequence