Experiment: LB_plus_SM_buffer with Ffm_phage 0.001875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4062 and GFF4063 are separated by 111 nucleotides GFF4063 and GFF4064 are separated by 3 nucleotides GFF4064 and GFF4065 overlap by 4 nucleotides
GFF4062 - Putative cytoplasmic protein USSDB7A, at 24,089 to 24,574
GFF4062
GFF4063 - Type VI secretion lipoprotein/VasD, at 24,686 to 25,177
GFF4063
GFF4064 - Uncharacterized protein ImpJ/VasE, at 25,181 to 26,524
GFF4064
GFF4065 - Outer membrane protein ImpK/VasF, OmpA/MotB domain, at 26,521 to 27,825
GFF4065
Position (kb) 25
26
27 Strain fitness (log2 ratio) -2
-1
0
1 at 24.269 kb on + strand, within GFF4062 at 24.270 kb on - strand, within GFF4062 at 24.270 kb on - strand, within GFF4062 at 24.302 kb on + strand, within GFF4062 at 24.829 kb on - strand, within GFF4063 at 25.156 kb on - strand at 25.322 kb on + strand, within GFF4064 at 25.327 kb on + strand, within GFF4064 at 25.611 kb on + strand, within GFF4064 at 25.724 kb on - strand, within GFF4064 at 25.753 kb on + strand, within GFF4064 at 25.754 kb on - strand, within GFF4064 at 25.780 kb on + strand, within GFF4064 at 26.009 kb on + strand, within GFF4064 at 26.009 kb on + strand, within GFF4064 at 26.009 kb on + strand, within GFF4064 at 26.010 kb on - strand, within GFF4064 at 26.043 kb on - strand, within GFF4064 at 26.052 kb on - strand, within GFF4064 at 26.052 kb on - strand, within GFF4064 at 26.165 kb on + strand, within GFF4064 at 26.165 kb on + strand, within GFF4064 at 26.399 kb on + strand at 26.399 kb on + strand at 26.400 kb on - strand at 26.400 kb on - strand at 26.402 kb on + strand at 26.469 kb on - strand at 26.589 kb on + strand at 26.831 kb on + strand, within GFF4065 at 26.832 kb on - strand, within GFF4065 at 26.873 kb on + strand, within GFF4065 at 26.889 kb on + strand, within GFF4065 at 27.051 kb on - strand, within GFF4065 at 27.060 kb on + strand, within GFF4065 at 27.065 kb on - strand, within GFF4065 at 27.136 kb on - strand, within GFF4065 at 27.307 kb on + strand, within GFF4065 at 27.351 kb on + strand, within GFF4065 at 27.352 kb on - strand, within GFF4065 at 27.352 kb on - strand, within GFF4065 at 27.478 kb on - strand, within GFF4065
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Ffm_phage 0.001875 MOI remove 24,269 + GFF4062 0.37 -1.5 24,270 - GFF4062 0.37 +0.2 24,270 - GFF4062 0.37 -0.1 24,302 + GFF4062 0.44 -0.1 24,829 - GFF4063 0.29 +0.3 25,156 - +0.3 25,322 + GFF4064 0.10 -0.5 25,327 + GFF4064 0.11 +0.2 25,611 + GFF4064 0.32 -0.0 25,724 - GFF4064 0.40 +0.7 25,753 + GFF4064 0.43 +0.6 25,754 - GFF4064 0.43 +0.4 25,780 + GFF4064 0.45 +0.5 26,009 + GFF4064 0.62 -0.1 26,009 + GFF4064 0.62 +1.5 26,009 + GFF4064 0.62 -0.0 26,010 - GFF4064 0.62 +0.4 26,043 - GFF4064 0.64 -0.1 26,052 - GFF4064 0.65 +0.1 26,052 - GFF4064 0.65 +0.4 26,165 + GFF4064 0.73 -0.4 26,165 + GFF4064 0.73 -0.0 26,399 + -0.0 26,399 + +0.0 26,400 - -1.8 26,400 - +0.0 26,402 + -0.4 26,469 - +0.6 26,589 + +0.0 26,831 + GFF4065 0.24 +0.9 26,832 - GFF4065 0.24 -0.5 26,873 + GFF4065 0.27 +0.2 26,889 + GFF4065 0.28 -0.3 27,051 - GFF4065 0.41 -0.1 27,060 + GFF4065 0.41 +0.1 27,065 - GFF4065 0.42 +0.1 27,136 - GFF4065 0.47 +0.3 27,307 + GFF4065 0.60 -0.8 27,351 + GFF4065 0.64 -0.2 27,352 - GFF4065 0.64 -0.2 27,352 - GFF4065 0.64 -0.2 27,478 - GFF4065 0.73 -1.0
Or see this region's nucleotide sequence