Experiment: Nickel (II) chloride 0.8 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_3767 and rpmG are separated by 305 nucleotides rpmG and rpmB are separated by 6 nucleotides rpmB and radC are separated by 122 nucleotides radC and Shewana3_3771 are separated by 121 nucleotides
Shewana3_3767: Shewana3_3767 - N-acetylglutamate synthase (RefSeq), at 4,528,145 to 4,529,482
_3767
Shewana3_3768: rpmG - 50S ribosomal protein L33 (RefSeq), at 4,529,788 to 4,529,961
rpmG
Shewana3_3769: rpmB - 50S ribosomal protein L28 (RefSeq), at 4,529,968 to 4,530,204
rpmB
Shewana3_3770: radC - DNA repair protein RadC (RefSeq), at 4,530,327 to 4,531,004
radC
Shewana3_3771: Shewana3_3771 - phosphopantothenoylcysteine decarboxylase / phosphopantothenate-cysteine ligase (RefSeq), at 4,531,126 to 4,532,355
_3771
Position (kb)
4530
4531
4532 Strain fitness (log2 ratio)
-1
0
1 at 4529.449 kb on + strand at 4529.449 kb on + strand at 4529.478 kb on - strand at 4530.321 kb on - strand at 4530.404 kb on - strand, within radC at 4530.521 kb on + strand, within radC at 4530.521 kb on + strand, within radC at 4530.528 kb on + strand, within radC at 4530.529 kb on - strand, within radC at 4530.598 kb on + strand, within radC at 4530.606 kb on - strand, within radC at 4530.643 kb on - strand, within radC at 4530.643 kb on - strand, within radC at 4530.643 kb on - strand, within radC at 4530.643 kb on - strand, within radC at 4530.643 kb on - strand, within radC at 4530.754 kb on + strand, within radC at 4530.762 kb on - strand, within radC at 4530.973 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) chloride 0.8 mM remove 4,529,449 + -0.5 4,529,449 + +0.2 4,529,478 - -0.1 4,530,321 - -1.2 4,530,404 - radC Shewana3_3770 0.11 -1.2 4,530,521 + radC Shewana3_3770 0.29 -1.3 4,530,521 + radC Shewana3_3770 0.29 -0.6 4,530,528 + radC Shewana3_3770 0.30 -0.8 4,530,529 - radC Shewana3_3770 0.30 -1.1 4,530,598 + radC Shewana3_3770 0.40 -1.0 4,530,606 - radC Shewana3_3770 0.41 -0.8 4,530,643 - radC Shewana3_3770 0.47 -1.0 4,530,643 - radC Shewana3_3770 0.47 -1.3 4,530,643 - radC Shewana3_3770 0.47 -0.7 4,530,643 - radC Shewana3_3770 0.47 -0.7 4,530,643 - radC Shewana3_3770 0.47 -1.3 4,530,754 + radC Shewana3_3770 0.63 -0.4 4,530,762 - radC Shewana3_3770 0.64 -0.7 4,530,973 + +0.3
Or see this region's nucleotide sequence