Experiment: Nickel (II) chloride 0.8 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_1653 and bcp are separated by 180 nucleotides bcp and Shewana3_1655 are separated by 3 nucleotides Shewana3_1655 and Shewana3_1656 are separated by 172 nucleotides
Shewana3_1653: Shewana3_1653 - lysine exporter protein LysE/YggA (RefSeq), at 1,944,289 to 1,944,903
_1653
Shewana3_1654: bcp - thioredoxin-dependent thiol peroxidase (RefSeq), at 1,945,084 to 1,945,551
bcp
Shewana3_1655: Shewana3_1655 - glycine cleavage system transcriptional repressor, putative (RefSeq), at 1,945,555 to 1,946,106
_1655
Shewana3_1656: Shewana3_1656 - dihydrodipicolinate synthase (RefSeq), at 1,946,279 to 1,947,163
_1656
Position (kb)
1945
1946
1947 Strain fitness (log2 ratio)
-2
-1
0
1 at 1944.638 kb on + strand, within Shewana3_1653 at 1945.135 kb on - strand, within bcp at 1945.278 kb on + strand, within bcp at 1945.399 kb on + strand, within bcp at 1945.399 kb on + strand, within bcp at 1945.407 kb on - strand, within bcp at 1945.417 kb on - strand, within bcp at 1945.417 kb on - strand, within bcp at 1945.704 kb on - strand, within Shewana3_1655 at 1945.777 kb on - strand, within Shewana3_1655 at 1945.788 kb on + strand, within Shewana3_1655 at 1945.868 kb on - strand, within Shewana3_1655 at 1945.960 kb on - strand, within Shewana3_1655 at 1946.143 kb on + strand at 1946.174 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) chloride 0.8 mM remove 1,944,638 + Shewana3_1653 0.57 -0.3 1,945,135 - bcp Shewana3_1654 0.11 +0.5 1,945,278 + bcp Shewana3_1654 0.41 +0.7 1,945,399 + bcp Shewana3_1654 0.67 +0.7 1,945,399 + bcp Shewana3_1654 0.67 +0.7 1,945,407 - bcp Shewana3_1654 0.69 -0.1 1,945,417 - bcp Shewana3_1654 0.71 -0.4 1,945,417 - bcp Shewana3_1654 0.71 -0.3 1,945,704 - Shewana3_1655 0.27 -1.4 1,945,777 - Shewana3_1655 0.40 +0.7 1,945,788 + Shewana3_1655 0.42 -1.9 1,945,868 - Shewana3_1655 0.57 -2.3 1,945,960 - Shewana3_1655 0.73 -1.5 1,946,143 + -1.3 1,946,174 + +0.2
Or see this region's nucleotide sequence