Experiment: LB_plus_SM_buffer with 6F2_phage 0.01875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1643 and GFF1644 are separated by 1 nucleotides GFF1644 and GFF1645 are separated by 36 nucleotides GFF1645 and GFF1646 are separated by 29 nucleotides GFF1646 and GFF1647 are separated by 102 nucleotides
GFF1643 - hypothetical protein, at 29,048 to 29,647
GFF1643
GFF1644 - Crossover junction endodeoxyribonuclease RuvC (EC 3.1.22.4), at 29,649 to 30,170
GFF1644
GFF1645 - FIG000859: hypothetical protein YebC, at 30,207 to 30,947
GFF1645
GFF1646 - Dihydroneopterin triphosphate pyrophosphohydrolase type 2 (nudB), at 30,977 to 31,429
GFF1646
GFF1647 - Aspartyl-tRNA synthetase (EC 6.1.1.12), at 31,532 to 33,304
GFF1647
Position (kb)
30
31 Strain fitness (log2 ratio)
-1
0
1 at 29.249 kb on - strand, within GFF1643 at 29.378 kb on + strand, within GFF1643 at 29.420 kb on - strand, within GFF1643 at 29.423 kb on + strand, within GFF1643 at 29.526 kb on + strand, within GFF1643 at 29.549 kb on - strand, within GFF1643 at 30.455 kb on - strand, within GFF1645 at 30.455 kb on - strand, within GFF1645 at 30.657 kb on - strand, within GFF1645 at 30.689 kb on - strand, within GFF1645 at 30.689 kb on - strand, within GFF1645 at 30.933 kb on - strand at 30.933 kb on - strand at 30.936 kb on - strand at 31.089 kb on - strand, within GFF1646 at 31.520 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6F2_phage 0.01875 MOI remove 29,249 - GFF1643 0.34 +0.7 29,378 + GFF1643 0.55 -0.0 29,420 - GFF1643 0.62 -0.4 29,423 + GFF1643 0.62 -0.2 29,526 + GFF1643 0.80 -0.0 29,549 - GFF1643 0.83 -0.1 30,455 - GFF1645 0.33 -0.3 30,455 - GFF1645 0.33 -0.3 30,657 - GFF1645 0.61 -0.9 30,689 - GFF1645 0.65 -1.2 30,689 - GFF1645 0.65 -0.5 30,933 - +0.1 30,933 - -0.8 30,936 - -0.4 31,089 - GFF1646 0.25 -0.5 31,520 - +0.2
Or see this region's nucleotide sequence