Experiment: N2 fixation, H2 media
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS05810 and MMJJ_RS05815 overlap by 4 nucleotides MMJJ_RS05815 and MMJJ_RS05820 overlap by 11 nucleotides MMJJ_RS05820 and MMJJ_RS05825 are separated by 85 nucleotides MMJJ_RS05825 and MMJJ_RS05830 are separated by 158 nucleotides MMJJ_RS05830 and MMJJ_RS05835 are separated by 46 nucleotides
MMJJ_RS05810: MMJJ_RS05810 - precorrin-4 C(11)-methyltransferase, at 1,083,120 to 1,083,884
_RS05810
MMJJ_RS05815: MMJJ_RS05815 - hypothetical protein, at 1,083,881 to 1,084,078
_RS05815
MMJJ_RS05820: MMJJ_RS05820 - DUF2304 domain-containing protein, at 1,084,068 to 1,084,427
_RS05820
MMJJ_RS05825: MMJJ_RS05825 - aspartate carbamoyltransferase, at 1,084,513 to 1,085,421
_RS05825
MMJJ_RS05830: MMJJ_RS05830 - 30S ribosomal protein S8e, at 1,085,580 to 1,085,966
_RS05830
MMJJ_RS05835: MMJJ_RS05835 - bis-aminopropyl spermidine synthase family protein, at 1,086,013 to 1,087,185
_RS05835
Position (kb)
1084
1085
1086 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4
5
6
7
8
9
10
11
12 at 1083.520 kb on - strand, within MMJJ_RS05810 at 1083.523 kb on - strand, within MMJJ_RS05810 at 1083.523 kb on - strand at 1083.597 kb on + strand, within MMJJ_RS05810 at 1083.641 kb on - strand, within MMJJ_RS05810 at 1083.642 kb on + strand, within MMJJ_RS05810 at 1083.642 kb on + strand, within MMJJ_RS05810 at 1083.649 kb on - strand, within MMJJ_RS05810 at 1083.691 kb on - strand, within MMJJ_RS05810 at 1083.691 kb on - strand, within MMJJ_RS05810 at 1083.691 kb on - strand, within MMJJ_RS05810 at 1083.692 kb on + strand, within MMJJ_RS05810 at 1083.693 kb on - strand, within MMJJ_RS05810 at 1083.737 kb on + strand, within MMJJ_RS05810 at 1083.745 kb on - strand, within MMJJ_RS05810 at 1083.788 kb on - strand, within MMJJ_RS05810 at 1083.789 kb on + strand, within MMJJ_RS05810 at 1083.789 kb on + strand at 1083.790 kb on - strand, within MMJJ_RS05810 at 1083.790 kb on - strand at 1083.822 kb on - strand at 1083.823 kb on + strand at 1083.891 kb on + strand at 1083.899 kb on - strand at 1083.912 kb on + strand, within MMJJ_RS05815 at 1083.953 kb on + strand, within MMJJ_RS05815 at 1083.953 kb on + strand, within MMJJ_RS05815 at 1083.956 kb on - strand, within MMJJ_RS05815 at 1083.956 kb on - strand, within MMJJ_RS05815 at 1083.980 kb on - strand, within MMJJ_RS05815 at 1084.021 kb on - strand, within MMJJ_RS05815 at 1084.021 kb on - strand, within MMJJ_RS05815 at 1084.022 kb on + strand, within MMJJ_RS05815 at 1084.061 kb on - strand at 1084.062 kb on + strand at 1084.105 kb on - strand, within MMJJ_RS05820 at 1084.115 kb on - strand, within MMJJ_RS05820 at 1084.204 kb on + strand, within MMJJ_RS05820 at 1084.204 kb on + strand, within MMJJ_RS05820 at 1084.205 kb on - strand, within MMJJ_RS05820 at 1084.299 kb on - strand, within MMJJ_RS05820 at 1084.325 kb on - strand, within MMJJ_RS05820 at 1084.325 kb on - strand, within MMJJ_RS05820 at 1084.325 kb on - strand, within MMJJ_RS05820 at 1084.326 kb on + strand, within MMJJ_RS05820 at 1084.335 kb on + strand, within MMJJ_RS05820 at 1084.335 kb on + strand, within MMJJ_RS05820 at 1084.346 kb on - strand, within MMJJ_RS05820 at 1084.389 kb on - strand, within MMJJ_RS05820 at 1084.389 kb on - strand, within MMJJ_RS05820 at 1084.389 kb on - strand, within MMJJ_RS05820 at 1084.390 kb on + strand, within MMJJ_RS05820 at 1084.446 kb on - strand at 1084.446 kb on - strand at 1084.446 kb on - strand at 1084.447 kb on + strand at 1084.447 kb on + strand at 1084.447 kb on + strand at 1084.447 kb on + strand at 1084.447 kb on + strand at 1084.447 kb on + strand at 1084.447 kb on + strand at 1084.447 kb on + strand at 1084.447 kb on + strand at 1084.447 kb on + strand at 1084.447 kb on + strand at 1084.447 kb on + strand at 1084.447 kb on + strand at 1084.448 kb on - strand at 1084.448 kb on - strand at 1084.449 kb on + strand at 1084.450 kb on - strand at 1084.450 kb on - strand at 1084.450 kb on - strand at 1084.450 kb on - strand at 1084.451 kb on + strand at 1084.451 kb on + strand at 1084.451 kb on + strand at 1084.451 kb on + strand at 1084.455 kb on - strand at 1084.456 kb on + strand at 1084.456 kb on + strand at 1084.469 kb on + strand at 1084.470 kb on - strand at 1084.472 kb on - strand at 1084.473 kb on + strand at 1084.473 kb on + strand at 1084.473 kb on + strand at 1084.474 kb on - strand at 1084.484 kb on + strand at 1084.484 kb on + strand at 1084.485 kb on - strand at 1085.216 kb on + strand, within MMJJ_RS05825 at 1085.225 kb on - strand, within MMJJ_RS05825 at 1085.235 kb on + strand, within MMJJ_RS05825 at 1085.391 kb on + strand at 1085.423 kb on - strand at 1085.428 kb on - strand at 1085.432 kb on + strand at 1085.437 kb on - strand at 1085.438 kb on + strand at 1085.441 kb on - strand at 1085.441 kb on - strand at 1085.442 kb on + strand at 1085.447 kb on + strand at 1085.495 kb on + strand at 1085.525 kb on + strand at 1085.953 kb on - strand at 1085.964 kb on - strand at 1085.965 kb on + strand at 1086.025 kb on - strand at 1086.095 kb on + strand at 1086.140 kb on - strand, within MMJJ_RS05835 at 1086.141 kb on + strand, within MMJJ_RS05835 at 1086.144 kb on + strand, within MMJJ_RS05835 at 1086.149 kb on + strand, within MMJJ_RS05835 at 1086.189 kb on - strand, within MMJJ_RS05835 at 1086.197 kb on + strand, within MMJJ_RS05835 at 1086.249 kb on - strand, within MMJJ_RS05835 at 1086.250 kb on + strand, within MMJJ_RS05835 at 1086.266 kb on - strand, within MMJJ_RS05835 at 1086.274 kb on + strand, within MMJJ_RS05835 at 1086.299 kb on + strand, within MMJJ_RS05835 at 1086.338 kb on - strand, within MMJJ_RS05835 at 1086.371 kb on - strand, within MMJJ_RS05835 at 1086.372 kb on + strand, within MMJJ_RS05835 at 1086.372 kb on + strand, within MMJJ_RS05835 at 1086.376 kb on - strand, within MMJJ_RS05835
Per-strain Table
Position Strand Gene LocusTag Fraction N2 fixation, H2 media remove 1,083,520 - MMJJ_RS05810 0.52 -0.3 1,083,523 - MMJJ_RS05810 0.53 +1.0 1,083,523 - +1.4 1,083,597 + MMJJ_RS05810 0.62 -0.9 1,083,641 - MMJJ_RS05810 0.68 +1.0 1,083,642 + MMJJ_RS05810 0.68 +1.0 1,083,642 + MMJJ_RS05810 0.68 +0.8 1,083,649 - MMJJ_RS05810 0.69 -0.9 1,083,691 - MMJJ_RS05810 0.75 +0.1 1,083,691 - MMJJ_RS05810 0.75 +0.3 1,083,691 - MMJJ_RS05810 0.75 +0.8 1,083,692 + MMJJ_RS05810 0.75 +0.6 1,083,693 - MMJJ_RS05810 0.75 +3.0 1,083,737 + MMJJ_RS05810 0.81 +0.3 1,083,745 - MMJJ_RS05810 0.82 +1.2 1,083,788 - MMJJ_RS05810 0.87 -0.0 1,083,789 + MMJJ_RS05810 0.87 +0.8 1,083,789 + +2.0 1,083,790 - MMJJ_RS05810 0.88 -0.2 1,083,790 - +2.0 1,083,822 - +1.4 1,083,823 + -0.7 1,083,891 + +2.4 1,083,899 - +0.1 1,083,912 + MMJJ_RS05815 0.16 -0.3 1,083,953 + MMJJ_RS05815 0.36 +1.2 1,083,953 + MMJJ_RS05815 0.36 -1.6 1,083,956 - MMJJ_RS05815 0.38 -0.9 1,083,956 - MMJJ_RS05815 0.38 -0.7 1,083,980 - MMJJ_RS05815 0.50 -0.6 1,084,021 - MMJJ_RS05815 0.71 +0.9 1,084,021 - MMJJ_RS05815 0.71 +1.0 1,084,022 + MMJJ_RS05815 0.71 +1.0 1,084,061 - -0.6 1,084,062 + +1.2 1,084,105 - MMJJ_RS05820 0.10 -0.6 1,084,115 - MMJJ_RS05820 0.13 -0.3 1,084,204 + MMJJ_RS05820 0.38 +0.6 1,084,204 + MMJJ_RS05820 0.38 -0.7 1,084,205 - MMJJ_RS05820 0.38 +0.9 1,084,299 - MMJJ_RS05820 0.64 +0.8 1,084,325 - MMJJ_RS05820 0.71 -0.9 1,084,325 - MMJJ_RS05820 0.71 +1.5 1,084,325 - MMJJ_RS05820 0.71 +1.4 1,084,326 + MMJJ_RS05820 0.72 -1.6 1,084,335 + MMJJ_RS05820 0.74 +0.0 1,084,335 + MMJJ_RS05820 0.74 -0.7 1,084,346 - MMJJ_RS05820 0.77 -0.7 1,084,389 - MMJJ_RS05820 0.89 +0.4 1,084,389 - MMJJ_RS05820 0.89 -0.8 1,084,389 - MMJJ_RS05820 0.89 +0.5 1,084,390 + MMJJ_RS05820 0.89 +0.0 1,084,446 - +0.8 1,084,446 - +0.9 1,084,446 - +1.3 1,084,447 + +1.1 1,084,447 + -1.9 1,084,447 + +3.0 1,084,447 + +0.5 1,084,447 + +0.1 1,084,447 + -0.1 1,084,447 + -0.4 1,084,447 + -1.1 1,084,447 + +0.5 1,084,447 + -0.5 1,084,447 + +0.0 1,084,447 + -2.5 1,084,447 + +4.7 1,084,448 - +0.8 1,084,448 - +1.0 1,084,449 + +0.6 1,084,450 - -0.9 1,084,450 - +2.0 1,084,450 - +0.4 1,084,450 - +0.3 1,084,451 + +0.4 1,084,451 + -0.1 1,084,451 + -0.0 1,084,451 + -1.2 1,084,455 - +2.2 1,084,456 + -2.5 1,084,456 + +11.8 1,084,469 + +0.7 1,084,470 - +1.7 1,084,472 - +0.2 1,084,473 + +3.4 1,084,473 + +1.2 1,084,473 + -0.7 1,084,474 - +0.5 1,084,484 + +2.3 1,084,484 + -0.9 1,084,485 - +1.2 1,085,216 + MMJJ_RS05825 0.77 +0.1 1,085,225 - MMJJ_RS05825 0.78 +3.0 1,085,235 + MMJJ_RS05825 0.79 +0.7 1,085,391 + -0.4 1,085,423 - -0.1 1,085,428 - +0.0 1,085,432 + +0.3 1,085,437 - -1.1 1,085,438 + +0.6 1,085,441 - -0.7 1,085,441 - +0.3 1,085,442 + -2.1 1,085,447 + -0.3 1,085,495 + -0.5 1,085,525 + +10.4 1,085,953 - +1.7 1,085,964 - +0.1 1,085,965 + +2.5 1,086,025 - +2.1 1,086,095 + +1.2 1,086,140 - MMJJ_RS05835 0.11 -0.3 1,086,141 + MMJJ_RS05835 0.11 -0.3 1,086,144 + MMJJ_RS05835 0.11 +0.3 1,086,149 + MMJJ_RS05835 0.12 +3.8 1,086,189 - MMJJ_RS05835 0.15 -1.0 1,086,197 + MMJJ_RS05835 0.16 +0.4 1,086,249 - MMJJ_RS05835 0.20 -0.0 1,086,250 + MMJJ_RS05835 0.20 -0.2 1,086,266 - MMJJ_RS05835 0.22 +6.1 1,086,274 + MMJJ_RS05835 0.22 +0.9 1,086,299 + MMJJ_RS05835 0.24 +0.3 1,086,338 - MMJJ_RS05835 0.28 -1.6 1,086,371 - MMJJ_RS05835 0.31 -0.3 1,086,372 + MMJJ_RS05835 0.31 -0.3 1,086,372 + MMJJ_RS05835 0.31 +1.5 1,086,376 - MMJJ_RS05835 0.31 -0.5
Or see this region's nucleotide sequence