Experiment: Min media with glucose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS06930 and HSERO_RS06935 are separated by 174 nucleotides HSERO_RS06935 and HSERO_RS06940 are separated by 76 nucleotides HSERO_RS06940 and HSERO_RS06945 are separated by 335 nucleotides
HSERO_RS06930: HSERO_RS06930 - transcription elongation factor GreA, at 1,578,502 to 1,578,978
_RS06930
HSERO_RS06935: HSERO_RS06935 - RNA-binding protein, at 1,579,153 to 1,579,611
_RS06935
HSERO_RS06940: HSERO_RS06940 - 23S rRNA methyltransferase, at 1,579,688 to 1,580,335
_RS06940
HSERO_RS06945: HSERO_RS06945 - ATP-dependent metalloprotease, at 1,580,671 to 1,582,560
_RS06945
Position (kb)
1579
1580
1581 Strain fitness (log2 ratio)
-1
0
1 at 1578.967 kb on + strand at 1579.196 kb on + strand at 1579.196 kb on - strand at 1579.402 kb on - strand, within HSERO_RS06935 at 1579.476 kb on - strand, within HSERO_RS06935 at 1579.691 kb on + strand at 1579.743 kb on + strand at 1580.052 kb on + strand, within HSERO_RS06940 at 1580.052 kb on + strand, within HSERO_RS06940 at 1580.087 kb on + strand, within HSERO_RS06940 at 1580.304 kb on - strand at 1580.489 kb on + strand at 1580.574 kb on - strand at 1580.595 kb on + strand at 1580.603 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Min media with glucose remove 1,578,967 + -0.9 1,579,196 + -1.3 1,579,196 - +0.7 1,579,402 - HSERO_RS06935 0.54 +0.5 1,579,476 - HSERO_RS06935 0.70 +0.4 1,579,691 + +0.4 1,579,743 + -0.3 1,580,052 + HSERO_RS06940 0.56 -0.9 1,580,052 + HSERO_RS06940 0.56 -1.7 1,580,087 + HSERO_RS06940 0.62 -1.5 1,580,304 - +1.4 1,580,489 + +0.3 1,580,574 - +0.4 1,580,595 + -0.3 1,580,603 - -0.5
Or see this region's nucleotide sequence