Experiment: Min media with glucose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS04335 and HSERO_RS04340 overlap by 4 nucleotides HSERO_RS04340 and HSERO_RS04345 are separated by 196 nucleotides
HSERO_RS04335: HSERO_RS04335 - sodium:proton antiporter, at 957,794 to 959,002
_RS04335
HSERO_RS04340: HSERO_RS04340 - gamma-glutamyl ligase, at 958,999 to 960,228
_RS04340
HSERO_RS04345: HSERO_RS04345 - membrane protein, at 960,425 to 961,633
_RS04345
Position (kb) 958
959
960
961 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 958.202 kb on - strand, within HSERO_RS04335 at 958.322 kb on + strand, within HSERO_RS04335 at 958.395 kb on + strand, within HSERO_RS04335 at 958.995 kb on - strand at 959.039 kb on + strand at 959.071 kb on - strand at 959.078 kb on - strand at 959.083 kb on - strand at 959.100 kb on + strand at 959.282 kb on + strand, within HSERO_RS04340 at 959.379 kb on - strand, within HSERO_RS04340 at 959.462 kb on + strand, within HSERO_RS04340 at 959.548 kb on + strand, within HSERO_RS04340 at 959.604 kb on - strand, within HSERO_RS04340 at 959.704 kb on + strand, within HSERO_RS04340 at 959.921 kb on - strand, within HSERO_RS04340 at 959.942 kb on + strand, within HSERO_RS04340 at 960.200 kb on + strand at 960.287 kb on + strand at 960.295 kb on - strand at 960.390 kb on + strand at 960.390 kb on + strand at 960.390 kb on + strand at 960.535 kb on - strand at 960.553 kb on + strand, within HSERO_RS04345 at 960.694 kb on + strand, within HSERO_RS04345 at 960.786 kb on - strand, within HSERO_RS04345 at 960.830 kb on + strand, within HSERO_RS04345 at 960.830 kb on + strand, within HSERO_RS04345 at 961.060 kb on + strand, within HSERO_RS04345 at 961.093 kb on - strand, within HSERO_RS04345 at 961.209 kb on + strand, within HSERO_RS04345
Per-strain Table
Position Strand Gene LocusTag Fraction Min media with glucose remove 958,202 - HSERO_RS04335 0.34 -3.3 958,322 + HSERO_RS04335 0.44 -1.8 958,395 + HSERO_RS04335 0.50 -2.2 958,995 - -1.8 959,039 + -3.0 959,071 - -3.1 959,078 - -3.3 959,083 - -3.6 959,100 + -3.9 959,282 + HSERO_RS04340 0.23 -3.2 959,379 - HSERO_RS04340 0.31 -3.2 959,462 + HSERO_RS04340 0.38 -4.0 959,548 + HSERO_RS04340 0.45 -1.9 959,604 - HSERO_RS04340 0.49 -3.6 959,704 + HSERO_RS04340 0.57 -3.5 959,921 - HSERO_RS04340 0.75 -2.7 959,942 + HSERO_RS04340 0.77 -2.8 960,200 + -0.1 960,287 + +0.6 960,295 - +0.5 960,390 + -0.5 960,390 + +0.2 960,390 + +0.9 960,535 - -0.1 960,553 + HSERO_RS04345 0.11 +1.1 960,694 + HSERO_RS04345 0.22 -0.1 960,786 - HSERO_RS04345 0.30 -0.6 960,830 + HSERO_RS04345 0.33 +0.3 960,830 + HSERO_RS04345 0.33 -0.2 961,060 + HSERO_RS04345 0.53 -0.1 961,093 - HSERO_RS04345 0.55 -0.1 961,209 + HSERO_RS04345 0.65 -0.2
Or see this region's nucleotide sequence