Experiment: LB_plus_SM_buffer with ES18h1_phage 0.75 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2239 and GFF2240 are separated by 190 nucleotides GFF2240 and GFF2241 overlap by 7 nucleotides GFF2241 and GFF2242 overlap by 4 nucleotides GFF2242 and GFF2243 are separated by 44 nucleotides
GFF2239 - DeoR-type transcriptional regulator YihW, at 34,679 to 35,482
GFF2239
GFF2240 - Alpha-D-glucose-1-phosphatase, at 35,673 to 36,272
GFF2240
GFF2241 - Inner membrane protein YihY, formerly thought to be RNase BN, at 36,266 to 37,138
GFF2241
GFF2242 - D-tyrosyl-tRNA(Tyr) deacylase (EC 3.6.1.n1), at 37,135 to 37,572
GFF2242
GFF2243 - GNAT family acetyltransferase YiiD potentially involved in tRNA processing, at 37,617 to 38,558
GFF2243
Position (kb) 36
37
38 Strain fitness (log2 ratio) -1
0
1
2
3 at 35.297 kb on - strand, within GFF2239 at 35.530 kb on + strand at 35.611 kb on - strand at 35.643 kb on + strand at 35.679 kb on + strand at 35.680 kb on - strand at 35.680 kb on - strand at 35.731 kb on + strand at 35.731 kb on + strand at 35.731 kb on + strand at 35.732 kb on - strand at 35.883 kb on + strand, within GFF2240 at 35.914 kb on + strand, within GFF2240 at 35.946 kb on - strand, within GFF2240 at 35.954 kb on + strand, within GFF2240 at 36.000 kb on - strand, within GFF2240 at 36.067 kb on - strand, within GFF2240 at 36.067 kb on - strand, within GFF2240 at 36.182 kb on + strand, within GFF2240 at 36.183 kb on - strand, within GFF2240 at 36.230 kb on + strand at 36.265 kb on + strand at 36.331 kb on - strand at 36.358 kb on + strand, within GFF2241 at 36.359 kb on - strand, within GFF2241 at 36.359 kb on - strand, within GFF2241 at 36.359 kb on - strand, within GFF2241 at 36.396 kb on + strand, within GFF2241 at 36.397 kb on - strand, within GFF2241 at 36.406 kb on - strand, within GFF2241 at 36.502 kb on + strand, within GFF2241 at 36.502 kb on + strand, within GFF2241 at 36.536 kb on - strand, within GFF2241 at 36.617 kb on + strand, within GFF2241 at 36.618 kb on - strand, within GFF2241 at 36.618 kb on - strand, within GFF2241 at 36.677 kb on + strand, within GFF2241 at 36.693 kb on - strand, within GFF2241 at 36.804 kb on + strand, within GFF2241 at 36.805 kb on - strand, within GFF2241 at 36.860 kb on - strand, within GFF2241 at 36.957 kb on - strand, within GFF2241 at 36.958 kb on + strand, within GFF2241 at 36.989 kb on + strand, within GFF2241 at 37.030 kb on - strand, within GFF2241 at 37.045 kb on + strand, within GFF2241 at 37.045 kb on + strand, within GFF2241 at 37.046 kb on - strand, within GFF2241 at 37.046 kb on - strand, within GFF2241 at 37.046 kb on - strand, within GFF2241 at 37.046 kb on - strand, within GFF2241 at 37.047 kb on + strand, within GFF2241 at 37.068 kb on + strand at 37.145 kb on + strand at 37.224 kb on - strand, within GFF2242 at 37.294 kb on - strand, within GFF2242 at 37.332 kb on - strand, within GFF2242 at 37.386 kb on + strand, within GFF2242 at 37.487 kb on + strand, within GFF2242 at 37.537 kb on - strand at 37.569 kb on - strand at 37.611 kb on - strand at 37.611 kb on - strand at 37.620 kb on + strand at 37.780 kb on + strand, within GFF2243 at 37.801 kb on - strand, within GFF2243 at 37.802 kb on + strand, within GFF2243 at 37.803 kb on - strand, within GFF2243 at 37.835 kb on + strand, within GFF2243 at 37.835 kb on + strand, within GFF2243 at 37.836 kb on - strand, within GFF2243 at 37.843 kb on + strand, within GFF2243 at 37.843 kb on + strand, within GFF2243 at 37.844 kb on - strand, within GFF2243 at 38.000 kb on + strand, within GFF2243 at 38.000 kb on + strand, within GFF2243 at 38.001 kb on - strand, within GFF2243 at 38.003 kb on + strand, within GFF2243 at 38.003 kb on + strand, within GFF2243 at 38.043 kb on - strand, within GFF2243 at 38.050 kb on + strand, within GFF2243
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with ES18h1_phage 0.75 MOI remove 35,297 - GFF2239 0.77 -1.2 35,530 + +0.1 35,611 - -0.2 35,643 + +0.4 35,679 + +0.1 35,680 - +1.8 35,680 - -1.2 35,731 + -0.6 35,731 + +0.1 35,731 + +3.0 35,732 - +1.1 35,883 + GFF2240 0.35 +2.0 35,914 + GFF2240 0.40 -0.1 35,946 - GFF2240 0.46 -0.8 35,954 + GFF2240 0.47 +0.6 36,000 - GFF2240 0.55 +0.3 36,067 - GFF2240 0.66 -0.9 36,067 - GFF2240 0.66 -0.4 36,182 + GFF2240 0.85 -0.3 36,183 - GFF2240 0.85 -1.3 36,230 + -0.6 36,265 + +0.8 36,331 - +0.3 36,358 + GFF2241 0.11 +0.4 36,359 - GFF2241 0.11 -0.9 36,359 - GFF2241 0.11 +0.2 36,359 - GFF2241 0.11 -0.5 36,396 + GFF2241 0.15 +0.5 36,397 - GFF2241 0.15 +2.3 36,406 - GFF2241 0.16 +0.7 36,502 + GFF2241 0.27 -0.4 36,502 + GFF2241 0.27 +1.5 36,536 - GFF2241 0.31 +0.4 36,617 + GFF2241 0.40 +0.9 36,618 - GFF2241 0.40 +0.6 36,618 - GFF2241 0.40 +1.0 36,677 + GFF2241 0.47 +0.5 36,693 - GFF2241 0.49 -0.4 36,804 + GFF2241 0.62 +1.2 36,805 - GFF2241 0.62 -0.2 36,860 - GFF2241 0.68 -1.4 36,957 - GFF2241 0.79 +1.6 36,958 + GFF2241 0.79 +0.4 36,989 + GFF2241 0.83 -1.2 37,030 - GFF2241 0.88 +0.9 37,045 + GFF2241 0.89 +0.4 37,045 + GFF2241 0.89 -0.6 37,046 - GFF2241 0.89 +0.9 37,046 - GFF2241 0.89 -0.4 37,046 - GFF2241 0.89 +0.1 37,046 - GFF2241 0.89 -0.3 37,047 + GFF2241 0.89 +1.0 37,068 + -0.9 37,145 + +0.6 37,224 - GFF2242 0.20 +1.5 37,294 - GFF2242 0.36 -0.5 37,332 - GFF2242 0.45 -1.4 37,386 + GFF2242 0.57 -0.3 37,487 + GFF2242 0.80 +2.8 37,537 - -0.1 37,569 - -0.0 37,611 - +0.2 37,611 - -1.2 37,620 + +1.2 37,780 + GFF2243 0.17 +1.3 37,801 - GFF2243 0.20 -0.0 37,802 + GFF2243 0.20 -0.7 37,803 - GFF2243 0.20 -0.9 37,835 + GFF2243 0.23 -1.3 37,835 + GFF2243 0.23 +0.4 37,836 - GFF2243 0.23 -0.4 37,843 + GFF2243 0.24 +0.2 37,843 + GFF2243 0.24 -1.0 37,844 - GFF2243 0.24 +0.8 38,000 + GFF2243 0.41 -0.0 38,000 + GFF2243 0.41 -0.1 38,001 - GFF2243 0.41 +0.0 38,003 + GFF2243 0.41 +0.3 38,003 + GFF2243 0.41 +0.2 38,043 - GFF2243 0.45 -0.8 38,050 + GFF2243 0.46 -0.9
Or see this region's nucleotide sequence