Experiment: D-Cycloserine 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5897 and Pf1N1B4_5898 are separated by 173 nucleotides Pf1N1B4_5898 and Pf1N1B4_5899 are separated by 4 nucleotides Pf1N1B4_5899 and Pf1N1B4_5900 overlap by 29 nucleotides Pf1N1B4_5900 and Pf1N1B4_5901 are separated by 57 nucleotides
Pf1N1B4_5897 - FIG00963512: hypothetical protein, at 340,631 to 342,550
_5897
Pf1N1B4_5898 - hypothetical protein, at 342,724 to 343,332
_5898
Pf1N1B4_5899 - FIG00954746: hypothetical protein, at 343,337 to 344,551
_5899
Pf1N1B4_5900 - Cellobiose phosphotransferase system YdjC-like protein, at 344,523 to 345,335
_5900
Pf1N1B4_5901 - hypothetical protein, at 345,393 to 346,082
_5901
Position (kb)
343
344
345 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 342.358 kb on - strand, within Pf1N1B4_5897 at 342.368 kb on - strand at 342.437 kb on + strand at 342.438 kb on - strand at 342.643 kb on - strand at 342.643 kb on - strand at 342.721 kb on + strand at 342.721 kb on + strand at 342.905 kb on - strand, within Pf1N1B4_5898 at 342.919 kb on + strand, within Pf1N1B4_5898 at 342.920 kb on - strand, within Pf1N1B4_5898 at 342.991 kb on + strand at 343.158 kb on + strand, within Pf1N1B4_5898 at 343.158 kb on + strand, within Pf1N1B4_5898 at 343.159 kb on - strand, within Pf1N1B4_5898 at 343.161 kb on - strand, within Pf1N1B4_5898 at 343.430 kb on - strand at 343.887 kb on + strand, within Pf1N1B4_5899 at 343.896 kb on - strand, within Pf1N1B4_5899 at 344.157 kb on - strand, within Pf1N1B4_5899 at 344.180 kb on + strand, within Pf1N1B4_5899 at 344.722 kb on - strand, within Pf1N1B4_5900 at 345.011 kb on + strand, within Pf1N1B4_5900 at 345.094 kb on + strand, within Pf1N1B4_5900 at 345.094 kb on + strand, within Pf1N1B4_5900 at 345.094 kb on + strand, within Pf1N1B4_5900 at 345.094 kb on + strand, within Pf1N1B4_5900 at 345.094 kb on + strand, within Pf1N1B4_5900 at 345.094 kb on + strand, within Pf1N1B4_5900 at 345.094 kb on + strand, within Pf1N1B4_5900 at 345.094 kb on + strand, within Pf1N1B4_5900 at 345.094 kb on + strand, within Pf1N1B4_5900 at 345.095 kb on - strand, within Pf1N1B4_5900 at 345.095 kb on - strand, within Pf1N1B4_5900 at 345.095 kb on - strand, within Pf1N1B4_5900 at 345.095 kb on - strand, within Pf1N1B4_5900 at 345.095 kb on - strand, within Pf1N1B4_5900 at 345.195 kb on + strand, within Pf1N1B4_5900 at 345.195 kb on + strand at 345.195 kb on + strand, within Pf1N1B4_5900 at 345.196 kb on - strand, within Pf1N1B4_5900 at 345.316 kb on + strand at 345.317 kb on - strand at 345.471 kb on + strand, within Pf1N1B4_5901 at 345.471 kb on + strand, within Pf1N1B4_5901 at 345.472 kb on - strand at 345.477 kb on + strand, within Pf1N1B4_5901 at 345.478 kb on - strand, within Pf1N1B4_5901 at 345.478 kb on - strand, within Pf1N1B4_5901
Per-strain Table
Position Strand Gene LocusTag Fraction D-Cycloserine 0.1 mg/ml remove 342,358 - Pf1N1B4_5897 0.90 -1.4 342,368 - -0.4 342,437 + -1.0 342,438 - +0.1 342,643 - -3.0 342,643 - +0.9 342,721 + -1.5 342,721 + +2.4 342,905 - Pf1N1B4_5898 0.30 -2.4 342,919 + Pf1N1B4_5898 0.32 -2.0 342,920 - Pf1N1B4_5898 0.32 -0.8 342,991 + +0.1 343,158 + Pf1N1B4_5898 0.71 -1.2 343,158 + Pf1N1B4_5898 0.71 +1.1 343,159 - Pf1N1B4_5898 0.71 -0.9 343,161 - Pf1N1B4_5898 0.72 -2.0 343,430 - -0.4 343,887 + Pf1N1B4_5899 0.45 -1.0 343,896 - Pf1N1B4_5899 0.46 -2.0 344,157 - Pf1N1B4_5899 0.67 -0.4 344,180 + Pf1N1B4_5899 0.69 -1.8 344,722 - Pf1N1B4_5900 0.24 -0.4 345,011 + Pf1N1B4_5900 0.60 +0.1 345,094 + Pf1N1B4_5900 0.70 -0.0 345,094 + Pf1N1B4_5900 0.70 +0.3 345,094 + Pf1N1B4_5900 0.70 +0.9 345,094 + Pf1N1B4_5900 0.70 +0.6 345,094 + Pf1N1B4_5900 0.70 +0.6 345,094 + Pf1N1B4_5900 0.70 -1.0 345,094 + Pf1N1B4_5900 0.70 -1.4 345,094 + Pf1N1B4_5900 0.70 +1.6 345,094 + Pf1N1B4_5900 0.70 -1.7 345,095 - Pf1N1B4_5900 0.70 -2.0 345,095 - Pf1N1B4_5900 0.70 +0.7 345,095 - Pf1N1B4_5900 0.70 -1.7 345,095 - Pf1N1B4_5900 0.70 -0.3 345,095 - Pf1N1B4_5900 0.70 +0.8 345,195 + Pf1N1B4_5900 0.83 -1.4 345,195 + -0.4 345,195 + Pf1N1B4_5900 0.83 -1.4 345,196 - Pf1N1B4_5900 0.83 -0.4 345,316 + +0.1 345,317 - +0.6 345,471 + Pf1N1B4_5901 0.11 +1.6 345,471 + Pf1N1B4_5901 0.11 -0.0 345,472 - +1.1 345,477 + Pf1N1B4_5901 0.12 -1.0 345,478 - Pf1N1B4_5901 0.12 +1.3 345,478 - Pf1N1B4_5901 0.12 +0.9
Or see this region's nucleotide sequence