Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_858

Experiment: Fusidic 0.125 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_857 and Pf1N1B4_858 are separated by 28 nucleotidesPf1N1B4_858 and Pf1N1B4_859 are separated by 92 nucleotidesPf1N1B4_859 and Pf1N1B4_860 are separated by 14 nucleotidesPf1N1B4_860 and Pf1N1B4_861 overlap by 1 nucleotides Pf1N1B4_857 - Ribosomal RNA large subunit methyltransferase N (EC 2.1.1.-), at 986,591 to 987,739 _857 Pf1N1B4_858 - Nucleoside diphosphate kinase (EC 2.7.4.6), at 987,768 to 988,193 _858 Pf1N1B4_859 - Believed to be involved in assembly of Fe-S clusters, at 988,286 to 988,486 _859 Pf1N1B4_860 - Ferredoxin, 2Fe-2S, at 988,501 to 988,842 _860 Pf1N1B4_861 - Chaperone protein HscA, at 988,842 to 990,707 _861 Position (kb) 987 988 989Strain fitness (log2 ratio) -2 -1 0 1 2at 986.935 kb on - strand, within Pf1N1B4_857at 988.229 kb on - strandat 988.260 kb on - strandat 988.411 kb on - strand, within Pf1N1B4_859at 988.411 kb on - strand, within Pf1N1B4_859

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Per-strain Table

Position Strand Gene LocusTag Fraction Fusidic 0.125 mg/ml
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986,935 - Pf1N1B4_857 0.30 -1.8
988,229 - +0.4
988,260 - +2.7
988,411 - Pf1N1B4_859 0.62 +1.7
988,411 - Pf1N1B4_859 0.62 +0.2

Or see this region's nucleotide sequence