Experiment: Fusidic 0.125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3890 and Pf1N1B4_3891 are separated by 185 nucleotides Pf1N1B4_3891 and Pf1N1B4_3892 are separated by 203 nucleotides Pf1N1B4_3892 and Pf1N1B4_3893 are separated by 349 nucleotides
Pf1N1B4_3890 - LSU rRNA 2'-O-methyl-C2498 methyltransferase RlmM, at 4,319,982 to 4,321,055
_3890
Pf1N1B4_3891 - tRNA 5-methylaminomethyl-2-thiouridine synthase TusA, at 4,321,241 to 4,321,492
_3891
Pf1N1B4_3892 - Multi antimicrobial extrusion protein (Na(+)/drug antiporter), MATE family of MDR efflux pumps, at 4,321,696 to 4,323,096
_3892
Pf1N1B4_3893 - Erythronate-4-phosphate dehydrogenase (EC 1.1.1.290), at 4,323,446 to 4,324,588
_3893
Position (kb)
4321
4322
4323
4324 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 4320.900 kb on + strand, within Pf1N1B4_3890 at 4320.900 kb on + strand, within Pf1N1B4_3890 at 4320.900 kb on + strand, within Pf1N1B4_3890 at 4320.929 kb on + strand, within Pf1N1B4_3890 at 4320.987 kb on + strand at 4320.987 kb on + strand at 4321.033 kb on + strand at 4321.192 kb on + strand at 4321.193 kb on - strand at 4321.193 kb on - strand at 4321.235 kb on + strand at 4321.490 kb on + strand at 4321.504 kb on + strand at 4321.504 kb on + strand at 4321.505 kb on - strand at 4321.511 kb on + strand at 4321.511 kb on + strand at 4321.511 kb on + strand at 4321.511 kb on + strand at 4321.511 kb on + strand at 4321.511 kb on + strand at 4321.511 kb on + strand at 4321.511 kb on + strand at 4321.511 kb on + strand at 4321.511 kb on + strand at 4321.511 kb on + strand at 4321.511 kb on + strand at 4321.511 kb on + strand at 4321.511 kb on + strand at 4321.512 kb on - strand at 4321.512 kb on - strand at 4321.512 kb on - strand at 4321.512 kb on - strand at 4321.512 kb on - strand at 4321.512 kb on - strand at 4321.512 kb on - strand at 4321.512 kb on - strand at 4321.512 kb on - strand at 4321.512 kb on - strand at 4321.512 kb on - strand at 4321.513 kb on + strand at 4321.513 kb on + strand at 4321.513 kb on + strand at 4321.514 kb on - strand at 4321.514 kb on - strand at 4321.533 kb on + strand at 4321.534 kb on - strand at 4321.534 kb on - strand at 4321.534 kb on - strand at 4321.649 kb on + strand at 4321.649 kb on + strand at 4321.664 kb on - strand at 4321.664 kb on - strand at 4321.673 kb on + strand at 4321.673 kb on + strand at 4321.673 kb on + strand at 4321.674 kb on - strand at 4321.784 kb on - strand at 4321.865 kb on - strand, within Pf1N1B4_3892 at 4321.986 kb on - strand, within Pf1N1B4_3892 at 4322.034 kb on - strand, within Pf1N1B4_3892 at 4322.063 kb on - strand, within Pf1N1B4_3892 at 4322.090 kb on + strand, within Pf1N1B4_3892 at 4322.091 kb on - strand, within Pf1N1B4_3892 at 4322.537 kb on + strand, within Pf1N1B4_3892 at 4322.537 kb on + strand at 4322.538 kb on - strand, within Pf1N1B4_3892 at 4322.538 kb on - strand, within Pf1N1B4_3892 at 4322.538 kb on - strand, within Pf1N1B4_3892 at 4322.538 kb on - strand, within Pf1N1B4_3892 at 4322.538 kb on - strand at 4322.550 kb on + strand, within Pf1N1B4_3892 at 4322.551 kb on - strand, within Pf1N1B4_3892 at 4322.551 kb on - strand, within Pf1N1B4_3892 at 4322.633 kb on + strand, within Pf1N1B4_3892 at 4322.675 kb on + strand, within Pf1N1B4_3892 at 4322.675 kb on + strand, within Pf1N1B4_3892 at 4322.676 kb on - strand, within Pf1N1B4_3892 at 4322.676 kb on - strand, within Pf1N1B4_3892 at 4322.676 kb on - strand, within Pf1N1B4_3892 at 4323.149 kb on + strand at 4323.150 kb on - strand at 4323.195 kb on + strand at 4323.410 kb on + strand at 4323.410 kb on + strand at 4323.410 kb on + strand at 4323.410 kb on + strand at 4323.410 kb on + strand at 4323.410 kb on + strand at 4323.411 kb on - strand at 4323.411 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.125 mg/ml remove 4,320,900 + Pf1N1B4_3890 0.85 -2.6 4,320,900 + Pf1N1B4_3890 0.85 -2.3 4,320,900 + Pf1N1B4_3890 0.85 +1.7 4,320,929 + Pf1N1B4_3890 0.88 +1.3 4,320,987 + +3.0 4,320,987 + -0.5 4,321,033 + -1.3 4,321,192 + -2.3 4,321,193 - -0.6 4,321,193 - -1.1 4,321,235 + +1.7 4,321,490 + +2.3 4,321,504 + -1.9 4,321,504 + +0.1 4,321,505 - +0.1 4,321,511 + -1.1 4,321,511 + +1.3 4,321,511 + -1.3 4,321,511 + +0.7 4,321,511 + -0.3 4,321,511 + +1.7 4,321,511 + -0.1 4,321,511 + -0.3 4,321,511 + -2.3 4,321,511 + -1.6 4,321,511 + -1.6 4,321,511 + +0.8 4,321,511 + -0.3 4,321,511 + -0.3 4,321,512 - -0.3 4,321,512 - -1.2 4,321,512 - -0.9 4,321,512 - +2.0 4,321,512 - +0.4 4,321,512 - -0.9 4,321,512 - -0.1 4,321,512 - -1.3 4,321,512 - +0.3 4,321,512 - +0.2 4,321,512 - -2.6 4,321,513 + -1.3 4,321,513 + -0.3 4,321,513 + -0.1 4,321,514 - -0.6 4,321,514 - +1.3 4,321,533 + +0.5 4,321,534 - +0.7 4,321,534 - -0.9 4,321,534 - +0.7 4,321,649 + -0.9 4,321,649 + -1.1 4,321,664 - -0.3 4,321,664 - -0.7 4,321,673 + +0.7 4,321,673 + -0.1 4,321,673 + +1.7 4,321,674 - +0.3 4,321,784 - -1.1 4,321,865 - Pf1N1B4_3892 0.12 -1.6 4,321,986 - Pf1N1B4_3892 0.21 +1.5 4,322,034 - Pf1N1B4_3892 0.24 +0.7 4,322,063 - Pf1N1B4_3892 0.26 -1.6 4,322,090 + Pf1N1B4_3892 0.28 -1.3 4,322,091 - Pf1N1B4_3892 0.28 -1.3 4,322,537 + Pf1N1B4_3892 0.60 +0.7 4,322,537 + +1.5 4,322,538 - Pf1N1B4_3892 0.60 -1.3 4,322,538 - Pf1N1B4_3892 0.60 +0.9 4,322,538 - Pf1N1B4_3892 0.60 +0.5 4,322,538 - Pf1N1B4_3892 0.60 -0.7 4,322,538 - -1.3 4,322,550 + Pf1N1B4_3892 0.61 -1.3 4,322,551 - Pf1N1B4_3892 0.61 -1.3 4,322,551 - Pf1N1B4_3892 0.61 -2.3 4,322,633 + Pf1N1B4_3892 0.67 +0.4 4,322,675 + Pf1N1B4_3892 0.70 +2.5 4,322,675 + Pf1N1B4_3892 0.70 -2.6 4,322,676 - Pf1N1B4_3892 0.70 -1.3 4,322,676 - Pf1N1B4_3892 0.70 +0.0 4,322,676 - Pf1N1B4_3892 0.70 -0.1 4,323,149 + -1.9 4,323,150 - -0.0 4,323,195 + -0.8 4,323,410 + -0.9 4,323,410 + -2.6 4,323,410 + +0.3 4,323,410 + +0.7 4,323,410 + -0.3 4,323,410 + +1.5 4,323,411 - +1.4 4,323,411 - -0.6
Or see this region's nucleotide sequence