Experiment: Fusidic 0.125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_248 and Pf1N1B4_249 are separated by 67 nucleotides Pf1N1B4_249 and Pf1N1B4_250 are separated by 181 nucleotides Pf1N1B4_250 and Pf1N1B4_251 are separated by 256 nucleotides
Pf1N1B4_248 - EAL-domain protein, at 304,330 to 305,493
_248
Pf1N1B4_249 - Deoxyguanosinetriphosphate triphosphohydrolase (EC 3.1.5.1), at 305,561 to 306,892
_249
Pf1N1B4_250 - response regulator, at 307,074 to 307,466
_250
Pf1N1B4_251 - DNA-binding response regulator, LuxR family, at 307,723 to 308,349
_251
Position (kb) 305
306
307 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 304.574 kb on + strand, within Pf1N1B4_248 at 304.575 kb on - strand, within Pf1N1B4_248 at 304.981 kb on + strand, within Pf1N1B4_248 at 304.986 kb on - strand, within Pf1N1B4_248 at 304.986 kb on - strand, within Pf1N1B4_248 at 304.986 kb on - strand, within Pf1N1B4_248 at 305.072 kb on - strand at 305.314 kb on - strand, within Pf1N1B4_248 at 305.338 kb on + strand at 305.339 kb on - strand, within Pf1N1B4_248 at 305.393 kb on - strand at 305.491 kb on + strand at 305.492 kb on - strand at 305.492 kb on - strand at 306.033 kb on + strand, within Pf1N1B4_249 at 306.033 kb on + strand, within Pf1N1B4_249 at 306.038 kb on - strand, within Pf1N1B4_249 at 306.038 kb on - strand at 306.038 kb on - strand, within Pf1N1B4_249 at 306.050 kb on + strand, within Pf1N1B4_249 at 306.084 kb on - strand, within Pf1N1B4_249 at 306.084 kb on - strand, within Pf1N1B4_249 at 306.084 kb on - strand, within Pf1N1B4_249 at 306.101 kb on + strand, within Pf1N1B4_249 at 306.101 kb on + strand, within Pf1N1B4_249 at 306.101 kb on + strand, within Pf1N1B4_249 at 306.111 kb on - strand, within Pf1N1B4_249 at 306.263 kb on + strand, within Pf1N1B4_249 at 306.264 kb on - strand, within Pf1N1B4_249 at 306.654 kb on - strand, within Pf1N1B4_249 at 306.745 kb on - strand, within Pf1N1B4_249 at 306.789 kb on + strand at 306.790 kb on - strand at 306.790 kb on - strand at 306.790 kb on - strand at 306.842 kb on + strand at 306.842 kb on + strand at 306.842 kb on + strand at 306.842 kb on + strand at 306.842 kb on + strand at 306.842 kb on + strand at 306.843 kb on - strand at 306.843 kb on - strand at 306.843 kb on - strand at 306.843 kb on - strand at 306.843 kb on - strand at 306.956 kb on + strand at 306.957 kb on - strand at 306.982 kb on - strand at 307.030 kb on + strand at 307.031 kb on - strand at 307.073 kb on + strand at 307.073 kb on + strand at 307.074 kb on - strand at 307.074 kb on - strand at 307.074 kb on - strand at 307.291 kb on - strand, within Pf1N1B4_250 at 307.325 kb on - strand, within Pf1N1B4_250 at 307.555 kb on - strand at 307.593 kb on + strand at 307.597 kb on + strand at 307.644 kb on + strand at 307.738 kb on - strand at 307.799 kb on - strand, within Pf1N1B4_251
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.125 mg/ml remove 304,574 + Pf1N1B4_248 0.21 -0.8 304,575 - Pf1N1B4_248 0.21 +0.2 304,981 + Pf1N1B4_248 0.56 +0.0 304,986 - Pf1N1B4_248 0.56 +1.1 304,986 - Pf1N1B4_248 0.56 +0.4 304,986 - Pf1N1B4_248 0.56 +0.2 305,072 - -1.8 305,314 - Pf1N1B4_248 0.85 -1.8 305,338 + +1.8 305,339 - Pf1N1B4_248 0.87 -0.2 305,393 - +0.3 305,491 + -0.9 305,492 - +0.5 305,492 - +2.2 306,033 + Pf1N1B4_249 0.35 -1.1 306,033 + Pf1N1B4_249 0.35 +0.8 306,038 - Pf1N1B4_249 0.36 -0.2 306,038 - -0.2 306,038 - Pf1N1B4_249 0.36 -1.8 306,050 + Pf1N1B4_249 0.37 -1.2 306,084 - Pf1N1B4_249 0.39 -0.1 306,084 - Pf1N1B4_249 0.39 -1.4 306,084 - Pf1N1B4_249 0.39 +0.5 306,101 + Pf1N1B4_249 0.41 +0.0 306,101 + Pf1N1B4_249 0.41 +0.2 306,101 + Pf1N1B4_249 0.41 -0.2 306,111 - Pf1N1B4_249 0.41 -1.2 306,263 + Pf1N1B4_249 0.53 +0.7 306,264 - Pf1N1B4_249 0.53 -1.2 306,654 - Pf1N1B4_249 0.82 +1.3 306,745 - Pf1N1B4_249 0.89 -0.7 306,789 + +1.4 306,790 - -2.2 306,790 - -0.2 306,790 - -1.8 306,842 + -0.5 306,842 + -0.2 306,842 + +1.4 306,842 + -0.8 306,842 + -1.2 306,842 + +1.4 306,843 - -0.2 306,843 - -1.5 306,843 - -1.2 306,843 - +1.4 306,843 - -1.2 306,956 + -1.2 306,957 - +0.8 306,982 - -2.5 307,030 + +0.3 307,031 - -2.2 307,073 + -2.2 307,073 + +1.2 307,074 - -2.8 307,074 - -3.0 307,074 - +1.2 307,291 - Pf1N1B4_250 0.55 -0.5 307,325 - Pf1N1B4_250 0.64 +0.4 307,555 - -2.5 307,593 + -0.8 307,597 + -2.2 307,644 + -2.2 307,738 - -0.2 307,799 - Pf1N1B4_251 0.12 -0.5
Or see this region's nucleotide sequence