Experiment: Fusidic 0.125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1447 and Pf1N1B4_1448 are separated by 464 nucleotides Pf1N1B4_1448 and Pf1N1B4_1449 are separated by 160 nucleotides
Pf1N1B4_1447 - Hydroxymethylpyrimidine phosphate synthase ThiC (EC 4.1.99.17), at 1,658,551 to 1,660,440
_1447
Pf1N1B4_1448 - Type I secretion outer membrane protein, TolC precursor, at 1,660,905 to 1,662,344
_1448
Pf1N1B4_1449 - 3-deoxy-D-manno-octulosonic-acid transferase (EC 2.-.-.-), at 1,662,505 to 1,663,785
_1449
Position (kb)
1660
1661
1662
1663 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 1659.971 kb on + strand, within Pf1N1B4_1447 at 1659.971 kb on + strand, within Pf1N1B4_1447 at 1659.971 kb on + strand, within Pf1N1B4_1447 at 1659.972 kb on - strand, within Pf1N1B4_1447 at 1659.993 kb on + strand, within Pf1N1B4_1447 at 1659.994 kb on - strand, within Pf1N1B4_1447 at 1660.008 kb on - strand, within Pf1N1B4_1447 at 1660.223 kb on + strand, within Pf1N1B4_1447 at 1660.223 kb on + strand, within Pf1N1B4_1447 at 1660.223 kb on + strand, within Pf1N1B4_1447 at 1660.224 kb on - strand, within Pf1N1B4_1447 at 1660.241 kb on + strand at 1660.241 kb on + strand, within Pf1N1B4_1447 at 1660.241 kb on + strand, within Pf1N1B4_1447 at 1660.241 kb on + strand, within Pf1N1B4_1447 at 1660.241 kb on + strand, within Pf1N1B4_1447 at 1660.242 kb on - strand, within Pf1N1B4_1447 at 1660.242 kb on - strand, within Pf1N1B4_1447 at 1660.242 kb on - strand, within Pf1N1B4_1447 at 1660.242 kb on - strand at 1660.242 kb on - strand, within Pf1N1B4_1447 at 1660.242 kb on - strand, within Pf1N1B4_1447 at 1660.346 kb on + strand at 1660.346 kb on + strand at 1660.347 kb on - strand at 1660.495 kb on + strand at 1660.669 kb on + strand at 1660.687 kb on + strand at 1660.687 kb on + strand at 1660.687 kb on + strand at 1660.687 kb on + strand at 1660.688 kb on - strand at 1660.688 kb on - strand at 1660.721 kb on - strand at 1660.721 kb on - strand at 1660.809 kb on + strand at 1660.809 kb on + strand at 1660.809 kb on + strand at 1660.809 kb on + strand at 1660.810 kb on - strand at 1660.810 kb on - strand at 1660.810 kb on - strand at 1660.810 kb on - strand at 1660.810 kb on - strand at 1660.810 kb on - strand at 1661.196 kb on + strand, within Pf1N1B4_1448 at 1661.197 kb on - strand, within Pf1N1B4_1448 at 1661.565 kb on + strand, within Pf1N1B4_1448 at 1661.565 kb on + strand, within Pf1N1B4_1448 at 1661.566 kb on - strand, within Pf1N1B4_1448 at 1661.566 kb on - strand, within Pf1N1B4_1448 at 1661.682 kb on + strand, within Pf1N1B4_1448 at 1661.683 kb on - strand, within Pf1N1B4_1448 at 1661.683 kb on - strand, within Pf1N1B4_1448 at 1661.683 kb on - strand, within Pf1N1B4_1448 at 1661.683 kb on - strand, within Pf1N1B4_1448 at 1661.683 kb on - strand, within Pf1N1B4_1448 at 1661.683 kb on - strand, within Pf1N1B4_1448 at 1661.683 kb on - strand, within Pf1N1B4_1448 at 1661.683 kb on - strand, within Pf1N1B4_1448 at 1661.760 kb on + strand, within Pf1N1B4_1448 at 1661.760 kb on + strand, within Pf1N1B4_1448 at 1661.760 kb on + strand, within Pf1N1B4_1448 at 1661.761 kb on - strand, within Pf1N1B4_1448 at 1661.761 kb on - strand, within Pf1N1B4_1448 at 1661.875 kb on - strand, within Pf1N1B4_1448 at 1661.875 kb on - strand, within Pf1N1B4_1448 at 1662.135 kb on + strand, within Pf1N1B4_1448 at 1662.135 kb on + strand, within Pf1N1B4_1448 at 1662.135 kb on + strand, within Pf1N1B4_1448 at 1662.135 kb on + strand, within Pf1N1B4_1448 at 1662.135 kb on + strand, within Pf1N1B4_1448 at 1662.135 kb on + strand at 1662.136 kb on - strand, within Pf1N1B4_1448 at 1662.136 kb on - strand, within Pf1N1B4_1448 at 1662.136 kb on - strand, within Pf1N1B4_1448 at 1662.136 kb on - strand at 1662.136 kb on - strand, within Pf1N1B4_1448 at 1662.153 kb on + strand, within Pf1N1B4_1448 at 1662.153 kb on + strand, within Pf1N1B4_1448 at 1662.153 kb on + strand, within Pf1N1B4_1448 at 1662.153 kb on + strand, within Pf1N1B4_1448 at 1662.154 kb on - strand, within Pf1N1B4_1448 at 1662.154 kb on - strand, within Pf1N1B4_1448 at 1662.154 kb on - strand, within Pf1N1B4_1448 at 1662.154 kb on - strand, within Pf1N1B4_1448 at 1662.175 kb on - strand, within Pf1N1B4_1448 at 1662.175 kb on - strand, within Pf1N1B4_1448 at 1662.175 kb on - strand, within Pf1N1B4_1448 at 1662.264 kb on + strand at 1662.264 kb on + strand at 1662.342 kb on + strand at 1662.343 kb on - strand at 1662.425 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.125 mg/ml remove 1,659,971 + Pf1N1B4_1447 0.75 +0.5 1,659,971 + Pf1N1B4_1447 0.75 -0.8 1,659,971 + Pf1N1B4_1447 0.75 -0.5 1,659,972 - Pf1N1B4_1447 0.75 -0.1 1,659,993 + Pf1N1B4_1447 0.76 +0.0 1,659,994 - Pf1N1B4_1447 0.76 -1.1 1,660,008 - Pf1N1B4_1447 0.77 -1.4 1,660,223 + Pf1N1B4_1447 0.88 -0.5 1,660,223 + Pf1N1B4_1447 0.88 -1.1 1,660,223 + Pf1N1B4_1447 0.88 -1.1 1,660,224 - Pf1N1B4_1447 0.89 -1.9 1,660,241 + +0.5 1,660,241 + Pf1N1B4_1447 0.89 -0.7 1,660,241 + Pf1N1B4_1447 0.89 -1.4 1,660,241 + Pf1N1B4_1447 0.89 -1.1 1,660,241 + Pf1N1B4_1447 0.89 -0.5 1,660,242 - Pf1N1B4_1447 0.89 -0.5 1,660,242 - Pf1N1B4_1447 0.89 +1.7 1,660,242 - Pf1N1B4_1447 0.89 +0.8 1,660,242 - +0.5 1,660,242 - Pf1N1B4_1447 0.89 -0.5 1,660,242 - Pf1N1B4_1447 0.89 -1.9 1,660,346 + -0.5 1,660,346 + -2.1 1,660,347 - +0.2 1,660,495 + +0.8 1,660,669 + -1.9 1,660,687 + -0.8 1,660,687 + -2.5 1,660,687 + +0.5 1,660,687 + -1.1 1,660,688 - -1.1 1,660,688 - -2.1 1,660,721 - +0.5 1,660,721 - -0.5 1,660,809 + +2.1 1,660,809 + -0.5 1,660,809 + +2.3 1,660,809 + +0.1 1,660,810 - -0.5 1,660,810 - +0.1 1,660,810 - -1.1 1,660,810 - -0.9 1,660,810 - -0.5 1,660,810 - -0.5 1,661,196 + Pf1N1B4_1448 0.20 -0.7 1,661,197 - Pf1N1B4_1448 0.20 +0.7 1,661,565 + Pf1N1B4_1448 0.46 -2.1 1,661,565 + Pf1N1B4_1448 0.46 +1.7 1,661,566 - Pf1N1B4_1448 0.46 -0.0 1,661,566 - Pf1N1B4_1448 0.46 +0.1 1,661,682 + Pf1N1B4_1448 0.54 -0.5 1,661,683 - Pf1N1B4_1448 0.54 -1.1 1,661,683 - Pf1N1B4_1448 0.54 -1.9 1,661,683 - Pf1N1B4_1448 0.54 -1.1 1,661,683 - Pf1N1B4_1448 0.54 -0.7 1,661,683 - Pf1N1B4_1448 0.54 -0.9 1,661,683 - Pf1N1B4_1448 0.54 +2.1 1,661,683 - Pf1N1B4_1448 0.54 +1.1 1,661,683 - Pf1N1B4_1448 0.54 +1.7 1,661,760 + Pf1N1B4_1448 0.59 -0.5 1,661,760 + Pf1N1B4_1448 0.59 -1.5 1,661,760 + Pf1N1B4_1448 0.59 -0.5 1,661,761 - Pf1N1B4_1448 0.59 -2.1 1,661,761 - Pf1N1B4_1448 0.59 -0.9 1,661,875 - Pf1N1B4_1448 0.67 +0.1 1,661,875 - Pf1N1B4_1448 0.67 +0.2 1,662,135 + Pf1N1B4_1448 0.85 +1.5 1,662,135 + Pf1N1B4_1448 0.85 -1.1 1,662,135 + Pf1N1B4_1448 0.85 -1.5 1,662,135 + Pf1N1B4_1448 0.85 -1.1 1,662,135 + Pf1N1B4_1448 0.85 -0.5 1,662,135 + +3.8 1,662,136 - Pf1N1B4_1448 0.85 +1.2 1,662,136 - Pf1N1B4_1448 0.85 -0.9 1,662,136 - Pf1N1B4_1448 0.85 +0.3 1,662,136 - +0.5 1,662,136 - Pf1N1B4_1448 0.85 +1.0 1,662,153 + Pf1N1B4_1448 0.87 +0.6 1,662,153 + Pf1N1B4_1448 0.87 -1.9 1,662,153 + Pf1N1B4_1448 0.87 -1.5 1,662,153 + Pf1N1B4_1448 0.87 -0.2 1,662,154 - Pf1N1B4_1448 0.87 -2.3 1,662,154 - Pf1N1B4_1448 0.87 -1.5 1,662,154 - Pf1N1B4_1448 0.87 +0.5 1,662,154 - Pf1N1B4_1448 0.87 +2.3 1,662,175 - Pf1N1B4_1448 0.88 +1.7 1,662,175 - Pf1N1B4_1448 0.88 -0.8 1,662,175 - Pf1N1B4_1448 0.88 -0.5 1,662,264 + -2.5 1,662,264 + +0.2 1,662,342 + +0.7 1,662,343 - +0.5 1,662,425 - -0.5
Or see this region's nucleotide sequence