Experiment: LB_plus_SM_buffer with Ffm_phage 0.1875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4064 and GFF4065 overlap by 4 nucleotides GFF4065 and GFF4066 are separated by 4 nucleotides
GFF4064 - Uncharacterized protein ImpJ/VasE, at 25,181 to 26,524
GFF4064
GFF4065 - Outer membrane protein ImpK/VasF, OmpA/MotB domain, at 26,521 to 27,825
GFF4065
GFF4066 - Putative inner membrane protein, at 27,830 to 28,603
GFF4066
Position (kb) 26
27
28 Strain fitness (log2 ratio) -1
0
1 at 25.611 kb on + strand, within GFF4064 at 25.724 kb on - strand, within GFF4064 at 25.753 kb on + strand, within GFF4064 at 25.754 kb on - strand, within GFF4064 at 25.780 kb on + strand, within GFF4064 at 26.009 kb on + strand, within GFF4064 at 26.009 kb on + strand, within GFF4064 at 26.009 kb on + strand, within GFF4064 at 26.010 kb on - strand, within GFF4064 at 26.043 kb on - strand, within GFF4064 at 26.052 kb on - strand, within GFF4064 at 26.052 kb on - strand, within GFF4064 at 26.165 kb on + strand, within GFF4064 at 26.165 kb on + strand, within GFF4064 at 26.399 kb on + strand at 26.399 kb on + strand at 26.400 kb on - strand at 26.400 kb on - strand at 26.402 kb on + strand at 26.469 kb on - strand at 26.589 kb on + strand at 26.831 kb on + strand, within GFF4065 at 26.832 kb on - strand, within GFF4065 at 26.873 kb on + strand, within GFF4065 at 26.889 kb on + strand, within GFF4065 at 27.051 kb on - strand, within GFF4065 at 27.060 kb on + strand, within GFF4065 at 27.065 kb on - strand, within GFF4065 at 27.136 kb on - strand, within GFF4065 at 27.307 kb on + strand, within GFF4065 at 27.351 kb on + strand, within GFF4065 at 27.352 kb on - strand, within GFF4065 at 27.352 kb on - strand, within GFF4065 at 27.478 kb on - strand, within GFF4065 at 27.656 kb on + strand, within GFF4065 at 27.874 kb on - strand at 27.914 kb on + strand, within GFF4066 at 27.921 kb on + strand, within GFF4066 at 27.922 kb on - strand, within GFF4066 at 27.954 kb on + strand, within GFF4066 at 27.955 kb on - strand, within GFF4066 at 27.955 kb on - strand, within GFF4066 at 27.955 kb on - strand, within GFF4066 at 28.037 kb on - strand, within GFF4066 at 28.037 kb on - strand, within GFF4066 at 28.100 kb on + strand, within GFF4066 at 28.100 kb on + strand, within GFF4066 at 28.100 kb on + strand, within GFF4066 at 28.101 kb on - strand, within GFF4066 at 28.101 kb on - strand, within GFF4066 at 28.101 kb on - strand, within GFF4066 at 28.165 kb on + strand, within GFF4066 at 28.166 kb on - strand, within GFF4066 at 28.167 kb on + strand, within GFF4066 at 28.168 kb on - strand, within GFF4066 at 28.174 kb on - strand, within GFF4066 at 28.240 kb on - strand, within GFF4066 at 28.402 kb on + strand, within GFF4066 at 28.421 kb on + strand, within GFF4066 at 28.509 kb on - strand, within GFF4066 at 28.654 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Ffm_phage 0.1875 MOI remove 25,611 + GFF4064 0.32 -0.2 25,724 - GFF4064 0.40 +0.6 25,753 + GFF4064 0.43 +0.3 25,754 - GFF4064 0.43 -0.0 25,780 + GFF4064 0.45 +0.1 26,009 + GFF4064 0.62 +0.0 26,009 + GFF4064 0.62 -0.8 26,009 + GFF4064 0.62 +0.2 26,010 - GFF4064 0.62 +0.2 26,043 - GFF4064 0.64 +1.2 26,052 - GFF4064 0.65 -0.1 26,052 - GFF4064 0.65 -0.4 26,165 + GFF4064 0.73 -0.2 26,165 + GFF4064 0.73 -0.1 26,399 + -0.3 26,399 + -0.1 26,400 - -0.0 26,400 - -0.1 26,402 + +0.1 26,469 - +0.4 26,589 + -0.3 26,831 + GFF4065 0.24 +0.6 26,832 - GFF4065 0.24 -1.0 26,873 + GFF4065 0.27 +0.5 26,889 + GFF4065 0.28 -0.1 27,051 - GFF4065 0.41 -0.4 27,060 + GFF4065 0.41 +0.0 27,065 - GFF4065 0.42 -0.2 27,136 - GFF4065 0.47 -0.0 27,307 + GFF4065 0.60 -1.1 27,351 + GFF4065 0.64 -0.0 27,352 - GFF4065 0.64 -0.4 27,352 - GFF4065 0.64 +0.4 27,478 - GFF4065 0.73 -0.1 27,656 + GFF4065 0.87 -0.3 27,874 - -0.0 27,914 + GFF4066 0.11 +0.4 27,921 + GFF4066 0.12 +0.1 27,922 - GFF4066 0.12 -0.2 27,954 + GFF4066 0.16 -0.1 27,955 - GFF4066 0.16 +0.4 27,955 - GFF4066 0.16 +0.1 27,955 - GFF4066 0.16 +0.5 28,037 - GFF4066 0.27 -0.7 28,037 - GFF4066 0.27 +0.5 28,100 + GFF4066 0.35 +0.4 28,100 + GFF4066 0.35 +0.1 28,100 + GFF4066 0.35 -0.3 28,101 - GFF4066 0.35 -0.1 28,101 - GFF4066 0.35 +0.5 28,101 - GFF4066 0.35 -0.6 28,165 + GFF4066 0.43 +0.2 28,166 - GFF4066 0.43 -0.0 28,167 + GFF4066 0.44 -0.1 28,168 - GFF4066 0.44 -0.2 28,174 - GFF4066 0.44 +0.0 28,240 - GFF4066 0.53 +0.1 28,402 + GFF4066 0.74 +0.4 28,421 + GFF4066 0.76 +0.2 28,509 - GFF4066 0.88 -1.0 28,654 - -0.0
Or see this region's nucleotide sequence