Experiment: LB_plus_SM_buffer with Ffm_phage 0.1875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4021 and GFF4022 are separated by 297 nucleotides GFF4022 and GFF4023 overlap by 4 nucleotides GFF4023 and GFF4024 are separated by 185 nucleotides GFF4024 and GFF4025 are separated by 39 nucleotides
GFF4021 - FIG01200701: possible membrane protein, at 288,965 to 289,318
GFF4021
GFF4022 - UPF0033 protein YedF, at 289,616 to 289,849
GFF4022
GFF4023 - Putative transport system permease protein, at 289,846 to 291,051
GFF4023
GFF4024 - FIG00640199: hypothetical protein, at 291,237 to 291,662
GFF4024
GFF4025 - Cytoplasmic alpha-amylase (EC 3.2.1.1), at 291,702 to 293,186
GFF4025
Position (kb) 289
290
291
292 Strain fitness (log2 ratio) -1
0
1
2 at 288.891 kb on + strand at 289.181 kb on - strand, within GFF4021 at 289.930 kb on + strand at 289.941 kb on - strand at 289.986 kb on + strand, within GFF4023 at 290.085 kb on - strand, within GFF4023 at 290.200 kb on - strand, within GFF4023 at 290.228 kb on + strand, within GFF4023 at 290.270 kb on + strand, within GFF4023 at 290.326 kb on - strand, within GFF4023 at 290.614 kb on + strand, within GFF4023 at 290.750 kb on - strand, within GFF4023 at 290.855 kb on + strand, within GFF4023 at 290.856 kb on - strand, within GFF4023 at 290.960 kb on + strand at 291.123 kb on - strand at 291.123 kb on - strand at 291.129 kb on - strand at 291.496 kb on + strand, within GFF4024 at 291.525 kb on + strand, within GFF4024 at 291.830 kb on - strand at 291.935 kb on + strand, within GFF4025 at 291.960 kb on + strand, within GFF4025 at 291.961 kb on - strand, within GFF4025
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Ffm_phage 0.1875 MOI remove 288,891 + +0.1 289,181 - GFF4021 0.61 +0.1 289,930 + +0.6 289,941 - -0.1 289,986 + GFF4023 0.12 +0.6 290,085 - GFF4023 0.20 +0.1 290,200 - GFF4023 0.29 +0.3 290,228 + GFF4023 0.32 +0.1 290,270 + GFF4023 0.35 +0.5 290,326 - GFF4023 0.40 +0.6 290,614 + GFF4023 0.64 +0.1 290,750 - GFF4023 0.75 +0.4 290,855 + GFF4023 0.84 +0.3 290,856 - GFF4023 0.84 +0.4 290,960 + -0.0 291,123 - +2.3 291,123 - +0.4 291,129 - +0.9 291,496 + GFF4024 0.61 +0.4 291,525 + GFF4024 0.68 +0.2 291,830 - +0.1 291,935 + GFF4025 0.16 -0.4 291,960 + GFF4025 0.17 -0.6 291,961 - GFF4025 0.17 +0.5
Or see this region's nucleotide sequence