Experiment: LB_plus_SM_buffer with 6F2_phage 0.1875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF712 and GFF713 are separated by 36 nucleotides GFF713 and GFF714 are separated by 242 nucleotides GFF714 and GFF715 overlap by 4 nucleotides GFF715 and GFF716 are separated by 43 nucleotides
GFF712 - hypothetical protein, at 68,757 to 68,885
GFF712
GFF713 - FIG01047037: hypothetical protein, at 68,922 to 69,092
GFF713
GFF714 - Mn-dependent transcriptional regulator MntR, at 69,335 to 69,808
GFF714
GFF715 - Putative membrane protein, at 69,805 to 70,917
GFF715
GFF716 - L,D-transpeptidase YbiS, at 70,961 to 71,881
GFF716
Position (kb) 69
70
71 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4
5 at 68.808 kb on - strand, within GFF712 at 68.808 kb on - strand, within GFF712 at 69.066 kb on - strand, within GFF713 at 69.077 kb on + strand at 69.078 kb on - strand at 69.129 kb on - strand at 69.129 kb on - strand at 69.149 kb on + strand at 69.149 kb on + strand at 69.150 kb on - strand at 69.151 kb on + strand at 69.151 kb on + strand at 69.201 kb on - strand at 69.201 kb on - strand at 69.201 kb on - strand at 69.205 kb on - strand at 69.211 kb on - strand at 69.212 kb on + strand at 69.278 kb on + strand at 69.278 kb on + strand at 69.390 kb on + strand, within GFF714 at 69.391 kb on - strand, within GFF714 at 69.391 kb on - strand, within GFF714 at 69.448 kb on - strand, within GFF714 at 69.456 kb on - strand, within GFF714 at 69.696 kb on + strand, within GFF714 at 69.938 kb on + strand, within GFF715 at 70.365 kb on - strand, within GFF715 at 70.368 kb on + strand, within GFF715 at 70.374 kb on + strand, within GFF715 at 70.430 kb on + strand, within GFF715 at 70.430 kb on + strand, within GFF715 at 70.432 kb on + strand, within GFF715 at 70.433 kb on - strand, within GFF715 at 70.433 kb on - strand, within GFF715 at 70.560 kb on + strand, within GFF715 at 70.573 kb on - strand, within GFF715 at 70.721 kb on - strand, within GFF715 at 70.721 kb on - strand, within GFF715 at 70.801 kb on - strand, within GFF715 at 70.801 kb on - strand, within GFF715 at 70.808 kb on + strand at 70.852 kb on + strand at 70.898 kb on + strand at 70.932 kb on + strand at 70.932 kb on + strand at 70.933 kb on - strand at 71.010 kb on - strand at 71.054 kb on - strand, within GFF716 at 71.059 kb on + strand, within GFF716 at 71.063 kb on - strand, within GFF716 at 71.136 kb on + strand, within GFF716 at 71.250 kb on - strand, within GFF716 at 71.310 kb on + strand, within GFF716 at 71.311 kb on - strand, within GFF716 at 71.488 kb on + strand, within GFF716 at 71.547 kb on + strand, within GFF716 at 71.550 kb on + strand, within GFF716 at 71.551 kb on - strand, within GFF716 at 71.734 kb on + strand, within GFF716 at 71.911 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6F2_phage 0.1875 MOI remove 68,808 - GFF712 0.40 -0.8 68,808 - GFF712 0.40 -1.0 69,066 - GFF713 0.84 -0.3 69,077 + +0.2 69,078 - -0.8 69,129 - +0.4 69,129 - +0.3 69,149 + +1.6 69,149 + -0.8 69,150 - +0.2 69,151 + +0.0 69,151 + -0.6 69,201 - +0.5 69,201 - +0.2 69,201 - +0.3 69,205 - -2.8 69,211 - -0.8 69,212 + +0.2 69,278 + +0.6 69,278 + +0.0 69,390 + GFF714 0.12 +0.0 69,391 - GFF714 0.12 -0.5 69,391 - GFF714 0.12 +1.0 69,448 - GFF714 0.24 -0.4 69,456 - GFF714 0.26 +0.0 69,696 + GFF714 0.76 -0.3 69,938 + GFF715 0.12 +0.2 70,365 - GFF715 0.50 -0.8 70,368 + GFF715 0.51 +1.4 70,374 + GFF715 0.51 +0.9 70,430 + GFF715 0.56 +0.8 70,430 + GFF715 0.56 -0.4 70,432 + GFF715 0.56 +0.1 70,433 - GFF715 0.56 +0.7 70,433 - GFF715 0.56 +0.1 70,560 + GFF715 0.68 +0.7 70,573 - GFF715 0.69 +2.4 70,721 - GFF715 0.82 -0.6 70,721 - GFF715 0.82 +2.9 70,801 - GFF715 0.89 -2.3 70,801 - GFF715 0.89 +0.5 70,808 + +0.1 70,852 + +0.1 70,898 + -0.1 70,932 + -1.0 70,932 + +0.1 70,933 - -0.5 71,010 - -0.7 71,054 - GFF716 0.10 -0.8 71,059 + GFF716 0.11 -0.2 71,063 - GFF716 0.11 +4.8 71,136 + GFF716 0.19 -0.3 71,250 - GFF716 0.31 -1.4 71,310 + GFF716 0.38 +0.4 71,311 - GFF716 0.38 +1.4 71,488 + GFF716 0.57 -0.4 71,547 + GFF716 0.64 +0.2 71,550 + GFF716 0.64 -0.9 71,551 - GFF716 0.64 -0.3 71,734 + GFF716 0.84 +2.7 71,911 - -2.7
Or see this region's nucleotide sequence