Experiment: LB_plus_SM_buffer with 6F2_phage 1.875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF686 and GFF687 are separated by 2 nucleotides GFF687 and GFF688 overlap by 1 nucleotides GFF688 and GFF689 overlap by 4 nucleotides GFF689 and GFF690 are separated by 132 nucleotides
GFF686 - Putative inner membrane protein, at 42,651 to 43,211
GFF686
GFF687 - Inner membrane protein YbhQ, at 43,214 to 44,176
GFF687
GFF688 - Cardiolipin synthetase (EC 2.7.8.-), at 44,176 to 45,417
GFF688
GFF689 - Endonuclease/Exonuclease/phosphatase family protein, at 45,414 to 46,172
GFF689
GFF690 - Putative inner membrane protein, at 46,305 to 46,715
GFF690
Position (kb)
44
45
46 Strain fitness (log2 ratio)
-1
0
1
2
3 at 43.363 kb on + strand, within GFF687 at 43.363 kb on + strand, within GFF687 at 43.609 kb on + strand, within GFF687 at 43.610 kb on - strand, within GFF687 at 43.644 kb on + strand, within GFF687 at 43.645 kb on - strand, within GFF687 at 43.645 kb on - strand, within GFF687 at 43.654 kb on + strand, within GFF687 at 43.738 kb on + strand, within GFF687 at 43.763 kb on - strand, within GFF687 at 43.783 kb on + strand, within GFF687 at 43.810 kb on + strand, within GFF687 at 43.813 kb on + strand, within GFF687 at 43.814 kb on - strand, within GFF687 at 43.839 kb on + strand, within GFF687 at 43.900 kb on - strand, within GFF687 at 43.996 kb on + strand, within GFF687 at 44.022 kb on - strand, within GFF687 at 44.171 kb on - strand at 44.171 kb on - strand at 44.269 kb on - strand at 44.367 kb on + strand, within GFF688 at 44.367 kb on + strand, within GFF688 at 44.367 kb on + strand, within GFF688 at 44.367 kb on + strand, within GFF688 at 44.368 kb on - strand, within GFF688 at 44.368 kb on - strand, within GFF688 at 44.368 kb on - strand, within GFF688 at 44.368 kb on - strand, within GFF688 at 44.410 kb on + strand, within GFF688 at 44.574 kb on - strand, within GFF688 at 44.604 kb on + strand, within GFF688 at 44.605 kb on - strand, within GFF688 at 44.979 kb on - strand, within GFF688 at 45.013 kb on - strand, within GFF688 at 45.013 kb on - strand, within GFF688 at 45.031 kb on - strand, within GFF688 at 45.031 kb on - strand, within GFF688 at 45.051 kb on + strand, within GFF688 at 45.097 kb on - strand, within GFF688 at 45.215 kb on - strand, within GFF688 at 45.217 kb on + strand, within GFF688 at 45.217 kb on + strand, within GFF688 at 45.217 kb on + strand, within GFF688 at 45.230 kb on - strand, within GFF688 at 45.518 kb on + strand, within GFF689 at 45.519 kb on - strand, within GFF689 at 45.589 kb on - strand, within GFF689 at 45.712 kb on - strand, within GFF689 at 45.712 kb on - strand, within GFF689 at 45.751 kb on - strand, within GFF689 at 45.802 kb on + strand, within GFF689 at 45.907 kb on + strand, within GFF689 at 46.208 kb on - strand at 46.208 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6F2_phage 1.875 MOI remove 43,363 + GFF687 0.15 -0.2 43,363 + GFF687 0.15 +0.3 43,609 + GFF687 0.41 -0.6 43,610 - GFF687 0.41 -0.3 43,644 + GFF687 0.45 +0.3 43,645 - GFF687 0.45 -0.3 43,645 - GFF687 0.45 +0.2 43,654 + GFF687 0.46 +0.2 43,738 + GFF687 0.54 +0.1 43,763 - GFF687 0.57 +0.3 43,783 + GFF687 0.59 -0.0 43,810 + GFF687 0.62 -0.0 43,813 + GFF687 0.62 +2.6 43,814 - GFF687 0.62 -0.4 43,839 + GFF687 0.65 -1.6 43,900 - GFF687 0.71 -0.2 43,996 + GFF687 0.81 -0.4 44,022 - GFF687 0.84 +0.2 44,171 - +1.8 44,171 - -0.1 44,269 - +1.0 44,367 + GFF688 0.15 +0.0 44,367 + GFF688 0.15 -0.3 44,367 + GFF688 0.15 +0.5 44,367 + GFF688 0.15 -0.0 44,368 - GFF688 0.15 -0.1 44,368 - GFF688 0.15 +0.3 44,368 - GFF688 0.15 +0.3 44,368 - GFF688 0.15 +0.2 44,410 + GFF688 0.19 +0.5 44,574 - GFF688 0.32 +1.1 44,604 + GFF688 0.34 +0.5 44,605 - GFF688 0.35 +0.3 44,979 - GFF688 0.65 -0.0 45,013 - GFF688 0.67 +0.9 45,013 - GFF688 0.67 +0.3 45,031 - GFF688 0.69 +0.2 45,031 - GFF688 0.69 -0.3 45,051 + GFF688 0.70 -1.7 45,097 - GFF688 0.74 +0.2 45,215 - GFF688 0.84 +0.9 45,217 + GFF688 0.84 -0.9 45,217 + GFF688 0.84 +0.2 45,217 + GFF688 0.84 +0.7 45,230 - GFF688 0.85 +0.2 45,518 + GFF689 0.14 -1.5 45,519 - GFF689 0.14 +0.2 45,589 - GFF689 0.23 -0.3 45,712 - GFF689 0.39 +0.1 45,712 - GFF689 0.39 -0.7 45,751 - GFF689 0.44 +3.3 45,802 + GFF689 0.51 -0.5 45,907 + GFF689 0.65 +0.4 46,208 - +1.0 46,208 - -0.5
Or see this region's nucleotide sequence