Experiment: LB_plus_SM_buffer with 6F2_phage 1.875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3478 and GFF3479 are separated by 341 nucleotides GFF3479 and GFF3480 are separated by 41 nucleotides GFF3480 and GFF3481 are separated by 351 nucleotides
GFF3478 - NAD-dependent glyceraldehyde-3-phosphate dehydrogenase (EC 1.2.1.12), at 230,746 to 231,741
GFF3478
GFF3479 - Peptide methionine sulfoxide reductase MsrB (EC 1.8.4.12), at 232,083 to 232,496
GFF3479
GFF3480 - Uncharacterized protein YeaC, at 232,538 to 232,816
GFF3480
GFF3481 - Nicotinamidase (EC 3.5.1.19), at 233,168 to 233,824
GFF3481
Position (kb) 232
233 Strain fitness (log2 ratio) -2
-1
0
1 at 231.943 kb on + strand at 231.943 kb on + strand at 231.946 kb on + strand at 231.947 kb on - strand at 232.176 kb on - strand, within GFF3479 at 232.193 kb on + strand, within GFF3479 at 232.320 kb on + strand, within GFF3479 at 232.320 kb on + strand, within GFF3479 at 232.324 kb on + strand, within GFF3479 at 232.325 kb on - strand, within GFF3479 at 232.439 kb on + strand, within GFF3479 at 232.580 kb on + strand, within GFF3480 at 232.580 kb on + strand, within GFF3480 at 232.612 kb on + strand, within GFF3480 at 232.677 kb on + strand, within GFF3480 at 232.711 kb on + strand, within GFF3480 at 232.712 kb on - strand, within GFF3480 at 232.712 kb on - strand, within GFF3480 at 232.835 kb on + strand at 233.113 kb on + strand at 233.141 kb on + strand at 233.142 kb on - strand at 233.142 kb on - strand at 233.170 kb on - strand at 233.216 kb on - strand at 233.217 kb on + strand at 233.218 kb on - strand at 233.330 kb on + strand, within GFF3481 at 233.361 kb on + strand, within GFF3481 at 233.387 kb on - strand, within GFF3481 at 233.466 kb on + strand, within GFF3481 at 233.467 kb on - strand, within GFF3481 at 233.496 kb on + strand, within GFF3481
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6F2_phage 1.875 MOI remove 231,943 + +0.3 231,943 + -0.2 231,946 + -0.4 231,947 - -0.1 232,176 - GFF3479 0.22 -0.6 232,193 + GFF3479 0.27 +0.1 232,320 + GFF3479 0.57 -1.1 232,320 + GFF3479 0.57 -0.4 232,324 + GFF3479 0.58 -0.6 232,325 - GFF3479 0.58 -0.4 232,439 + GFF3479 0.86 -0.6 232,580 + GFF3480 0.15 -0.0 232,580 + GFF3480 0.15 -0.2 232,612 + GFF3480 0.27 -1.8 232,677 + GFF3480 0.50 +1.2 232,711 + GFF3480 0.62 +1.6 232,712 - GFF3480 0.62 +0.2 232,712 - GFF3480 0.62 -0.3 232,835 + +0.1 233,113 + +0.7 233,141 + -0.6 233,142 - +0.6 233,142 - +0.3 233,170 - -0.1 233,216 - -1.0 233,217 + -0.1 233,218 - -0.1 233,330 + GFF3481 0.25 +0.4 233,361 + GFF3481 0.29 -0.3 233,387 - GFF3481 0.33 -1.2 233,466 + GFF3481 0.45 +0.3 233,467 - GFF3481 0.46 +0.0 233,496 + GFF3481 0.50 -0.9
Or see this region's nucleotide sequence