Experiment: PYE with Vancomycin 0.007 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_00700 and CCNA_00701 are separated by 586 nucleotides CCNA_00701 and CCNA_03888 are separated by 144 nucleotides CCNA_03888 and CCNA_00703 are separated by 102 nucleotides CCNA_00703 and CCNA_00704 are separated by 42 nucleotides CCNA_00704 and CCNA_00705 are separated by 151 nucleotides
CCNA_00700: CCNA_00700 - TonB-dependent receptor, at 759,099 to 761,024
_00700
CCNA_00701: CCNA_00701 - cold shock protein CspB, at 761,611 to 761,820
_00701
CCNA_03888: CCNA_03888 - hypothetical protein, at 761,965 to 762,324
_03888
CCNA_00703: CCNA_00703 - hypothetical protein, at 762,427 to 762,633
_00703
CCNA_00704: CCNA_00704 - hypothetical protein, at 762,676 to 762,873
_00704
CCNA_00705: CCNA_00705 - hypothetical protein, at 763,025 to 763,315
_00705
Position (kb) 761
762
763 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 760.989 kb on + strand at 761.076 kb on - strand at 761.076 kb on - strand at 761.076 kb on - strand at 761.130 kb on - strand at 761.135 kb on + strand at 761.143 kb on - strand at 761.146 kb on + strand at 761.213 kb on + strand at 761.231 kb on + strand at 761.252 kb on + strand at 761.275 kb on - strand at 761.544 kb on - strand at 761.544 kb on - strand at 761.580 kb on - strand at 761.580 kb on - strand at 761.610 kb on + strand at 761.632 kb on - strand, within CCNA_00701 at 761.650 kb on + strand, within CCNA_00701 at 761.651 kb on + strand, within CCNA_00701 at 761.667 kb on + strand, within CCNA_00701 at 761.670 kb on - strand, within CCNA_00701 at 761.671 kb on + strand, within CCNA_00701 at 761.675 kb on - strand, within CCNA_00701 at 761.689 kb on - strand, within CCNA_00701 at 761.717 kb on + strand, within CCNA_00701 at 761.717 kb on + strand at 761.722 kb on + strand, within CCNA_00701 at 761.725 kb on - strand, within CCNA_00701 at 761.730 kb on - strand, within CCNA_00701 at 761.743 kb on + strand, within CCNA_00701 at 761.751 kb on + strand, within CCNA_00701 at 761.751 kb on - strand, within CCNA_00701 at 761.759 kb on - strand, within CCNA_00701 at 761.776 kb on - strand, within CCNA_00701 at 761.785 kb on + strand, within CCNA_00701 at 761.831 kb on + strand at 761.839 kb on - strand at 761.839 kb on - strand at 761.848 kb on + strand at 761.849 kb on - strand at 761.856 kb on - strand at 761.905 kb on - strand at 761.907 kb on + strand at 761.910 kb on - strand at 761.921 kb on + strand at 761.932 kb on + strand at 761.945 kb on - strand at 762.035 kb on + strand, within CCNA_03888 at 762.048 kb on + strand, within CCNA_03888 at 762.067 kb on + strand, within CCNA_03888 at 762.084 kb on + strand, within CCNA_03888 at 762.092 kb on - strand, within CCNA_03888 at 762.092 kb on - strand, within CCNA_03888 at 762.092 kb on - strand, within CCNA_03888 at 762.092 kb on - strand, within CCNA_03888 at 762.092 kb on - strand, within CCNA_03888 at 762.092 kb on - strand, within CCNA_03888 at 762.092 kb on - strand, within CCNA_03888 at 762.108 kb on + strand, within CCNA_03888 at 762.111 kb on - strand, within CCNA_03888 at 762.116 kb on - strand, within CCNA_03888 at 762.116 kb on - strand, within CCNA_03888 at 762.157 kb on - strand, within CCNA_03888 at 762.167 kb on - strand, within CCNA_03888 at 762.217 kb on + strand, within CCNA_03888 at 762.238 kb on - strand, within CCNA_03888 at 762.239 kb on + strand, within CCNA_03888 at 762.249 kb on + strand, within CCNA_03888 at 762.256 kb on + strand, within CCNA_03888 at 762.256 kb on + strand, within CCNA_03888 at 762.264 kb on - strand, within CCNA_03888 at 762.279 kb on + strand, within CCNA_03888 at 762.287 kb on - strand, within CCNA_03888 at 762.307 kb on + strand at 762.341 kb on + strand at 762.341 kb on + strand at 762.346 kb on + strand at 762.349 kb on - strand at 762.349 kb on - strand at 762.354 kb on - strand at 762.354 kb on - strand at 762.385 kb on - strand at 762.385 kb on - strand at 762.412 kb on + strand at 762.419 kb on + strand at 762.424 kb on + strand at 762.424 kb on + strand at 762.424 kb on + strand at 762.424 kb on + strand at 762.432 kb on - strand at 762.432 kb on - strand at 762.432 kb on - strand at 762.432 kb on - strand at 762.432 kb on - strand at 762.475 kb on - strand, within CCNA_00703 at 762.480 kb on + strand, within CCNA_00703 at 762.488 kb on - strand, within CCNA_00703 at 762.488 kb on - strand, within CCNA_00703 at 762.501 kb on - strand, within CCNA_00703 at 762.558 kb on - strand, within CCNA_00703 at 762.577 kb on + strand, within CCNA_00703 at 762.577 kb on + strand, within CCNA_00703 at 762.580 kb on - strand, within CCNA_00703 at 762.583 kb on + strand, within CCNA_00703 at 762.585 kb on - strand, within CCNA_00703 at 762.585 kb on - strand, within CCNA_00703 at 762.585 kb on - strand, within CCNA_00703 at 762.585 kb on - strand, within CCNA_00703 at 762.585 kb on - strand, within CCNA_00703 at 762.585 kb on - strand, within CCNA_00703 at 762.590 kb on - strand, within CCNA_00703 at 762.590 kb on - strand, within CCNA_00703 at 762.632 kb on + strand at 762.669 kb on - strand at 762.696 kb on + strand, within CCNA_00704 at 762.696 kb on + strand, within CCNA_00704 at 762.704 kb on - strand, within CCNA_00704 at 762.704 kb on - strand, within CCNA_00704 at 762.704 kb on - strand, within CCNA_00704 at 762.729 kb on + strand, within CCNA_00704 at 762.836 kb on - strand, within CCNA_00704 at 762.847 kb on - strand, within CCNA_00704 at 762.847 kb on - strand, within CCNA_00704 at 762.902 kb on - strand at 762.902 kb on - strand at 762.906 kb on + strand at 762.906 kb on + strand at 762.916 kb on + strand at 762.916 kb on + strand at 762.916 kb on + strand at 762.924 kb on - strand at 762.951 kb on + strand at 762.951 kb on + strand at 762.959 kb on - strand at 762.959 kb on - strand at 762.959 kb on - strand at 762.959 kb on - strand at 762.959 kb on - strand at 763.010 kb on + strand at 763.018 kb on - strand at 763.064 kb on + strand, within CCNA_00705 at 763.072 kb on - strand, within CCNA_00705 at 763.072 kb on - strand, within CCNA_00705 at 763.072 kb on - strand, within CCNA_00705 at 763.105 kb on - strand, within CCNA_00705 at 763.125 kb on + strand, within CCNA_00705 at 763.183 kb on - strand, within CCNA_00705 at 763.263 kb on + strand, within CCNA_00705 at 763.273 kb on + strand, within CCNA_00705 at 763.281 kb on - strand, within CCNA_00705 at 763.300 kb on - strand at 763.305 kb on - strand at 763.322 kb on - strand at 763.322 kb on - strand at 763.324 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with Vancomycin 0.007 mg/ml remove 760,989 + +1.8 761,076 - -1.2 761,076 - +2.5 761,076 - -0.2 761,130 - +0.8 761,135 + -1.1 761,143 - -0.5 761,146 + +1.5 761,213 + -0.2 761,231 + +1.8 761,252 + +0.8 761,275 - +2.8 761,544 - -0.4 761,544 - +1.8 761,580 - +0.5 761,580 - +1.2 761,610 + -0.4 761,632 - CCNA_00701 0.10 +1.8 761,650 + CCNA_00701 0.19 -1.9 761,651 + CCNA_00701 0.19 -1.2 761,667 + CCNA_00701 0.27 +1.6 761,670 - CCNA_00701 0.28 +3.4 761,671 + CCNA_00701 0.29 +0.6 761,675 - CCNA_00701 0.30 -0.5 761,689 - CCNA_00701 0.37 -1.1 761,717 + CCNA_00701 0.50 -0.7 761,717 + +1.2 761,722 + CCNA_00701 0.53 +0.2 761,725 - CCNA_00701 0.54 +1.2 761,730 - CCNA_00701 0.57 -0.7 761,743 + CCNA_00701 0.63 +0.0 761,751 + CCNA_00701 0.67 +0.1 761,751 - CCNA_00701 0.67 +0.8 761,759 - CCNA_00701 0.70 +1.2 761,776 - CCNA_00701 0.79 -0.1 761,785 + CCNA_00701 0.83 +2.5 761,831 + -0.5 761,839 - +1.8 761,839 - -1.6 761,848 + +2.8 761,849 - -0.4 761,856 - +0.3 761,905 - -0.9 761,907 + +1.0 761,910 - +0.2 761,921 + +0.2 761,932 + +0.5 761,945 - +0.0 762,035 + CCNA_03888 0.19 -0.9 762,048 + CCNA_03888 0.23 -2.2 762,067 + CCNA_03888 0.28 -1.4 762,084 + CCNA_03888 0.33 -1.8 762,092 - CCNA_03888 0.35 +0.9 762,092 - CCNA_03888 0.35 +0.8 762,092 - CCNA_03888 0.35 -1.9 762,092 - CCNA_03888 0.35 -2.1 762,092 - CCNA_03888 0.35 +0.5 762,092 - CCNA_03888 0.35 +1.2 762,092 - CCNA_03888 0.35 +0.2 762,108 + CCNA_03888 0.40 -0.7 762,111 - CCNA_03888 0.41 +0.8 762,116 - CCNA_03888 0.42 -0.5 762,116 - CCNA_03888 0.42 -0.7 762,157 - CCNA_03888 0.53 -0.5 762,167 - CCNA_03888 0.56 -0.8 762,217 + CCNA_03888 0.70 -0.4 762,238 - CCNA_03888 0.76 -0.2 762,239 + CCNA_03888 0.76 +0.5 762,249 + CCNA_03888 0.79 +0.2 762,256 + CCNA_03888 0.81 -0.7 762,256 + CCNA_03888 0.81 -1.0 762,264 - CCNA_03888 0.83 -0.4 762,279 + CCNA_03888 0.87 -1.1 762,287 - CCNA_03888 0.89 +0.1 762,307 + +1.0 762,341 + -0.0 762,341 + -0.5 762,346 + -2.1 762,349 - -0.4 762,349 - -1.0 762,354 - -1.4 762,354 - -1.5 762,385 - -1.1 762,385 - +0.4 762,412 + -0.9 762,419 + +0.5 762,424 + +1.2 762,424 + -1.0 762,424 + -1.1 762,424 + -1.1 762,432 - +0.2 762,432 - -0.1 762,432 - -0.1 762,432 - +1.4 762,432 - +0.5 762,475 - CCNA_00703 0.23 -1.3 762,480 + CCNA_00703 0.26 +0.2 762,488 - CCNA_00703 0.29 +0.8 762,488 - CCNA_00703 0.29 +0.1 762,501 - CCNA_00703 0.36 +0.1 762,558 - CCNA_00703 0.63 -0.7 762,577 + CCNA_00703 0.72 +0.2 762,577 + CCNA_00703 0.72 +0.2 762,580 - CCNA_00703 0.74 -0.7 762,583 + CCNA_00703 0.75 -0.2 762,585 - CCNA_00703 0.76 -0.5 762,585 - CCNA_00703 0.76 -0.1 762,585 - CCNA_00703 0.76 +0.2 762,585 - CCNA_00703 0.76 +0.8 762,585 - CCNA_00703 0.76 -0.2 762,585 - CCNA_00703 0.76 +0.8 762,590 - CCNA_00703 0.79 -0.7 762,590 - CCNA_00703 0.79 -0.8 762,632 + -0.5 762,669 - -0.2 762,696 + CCNA_00704 0.10 +1.1 762,696 + CCNA_00704 0.10 +0.1 762,704 - CCNA_00704 0.14 -2.6 762,704 - CCNA_00704 0.14 -2.3 762,704 - CCNA_00704 0.14 +2.2 762,729 + CCNA_00704 0.27 -0.2 762,836 - CCNA_00704 0.81 +0.5 762,847 - CCNA_00704 0.86 -0.9 762,847 - CCNA_00704 0.86 +0.5 762,902 - -1.5 762,902 - +0.5 762,906 + +0.6 762,906 + -1.1 762,916 + +1.6 762,916 + +1.1 762,916 + +1.1 762,924 - -0.9 762,951 + -1.1 762,951 + +0.9 762,959 - +2.8 762,959 - +0.1 762,959 - -1.6 762,959 - +0.3 762,959 - +2.8 763,010 + +0.5 763,018 - +1.0 763,064 + CCNA_00705 0.13 +0.2 763,072 - CCNA_00705 0.16 -0.5 763,072 - CCNA_00705 0.16 +1.5 763,072 - CCNA_00705 0.16 +0.4 763,105 - CCNA_00705 0.27 +0.5 763,125 + CCNA_00705 0.34 -1.0 763,183 - CCNA_00705 0.54 +0.5 763,263 + CCNA_00705 0.82 +0.8 763,273 + CCNA_00705 0.85 -0.1 763,281 - CCNA_00705 0.88 +1.1 763,300 - +0.8 763,305 - +0.4 763,322 - +0.8 763,322 - +0.8 763,324 - +0.4
Or see this region's nucleotide sequence