Experiment: KB with Nitrofurantoin 0.0625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_3946 and Psyr_3947 overlap by 4 nucleotides Psyr_3947 and Psyr_3948 are separated by 8 nucleotides Psyr_3948 and Psyr_3949 are separated by 59 nucleotides Psyr_3949 and Psyr_3950 are separated by 60 nucleotides
Psyr_3946: Psyr_3946 - Proteobacterial methyltransferase, at 4,694,539 to 4,695,282
_3946
Psyr_3947: Psyr_3947 - Methyltransferase, putative, at 4,695,279 to 4,696,238
_3947
Psyr_3948: Psyr_3948 - pyridoxine 5'-phosphate synthase, at 4,696,247 to 4,696,987
_3948
Psyr_3949: Psyr_3949 - DNA replication and repair protein RecO, at 4,697,047 to 4,697,730
_3949
Psyr_3950: Psyr_3950 - Small GTP-binding protein domain:GTP-binding:GTP-binding protein Era, at 4,697,791 to 4,698,693
_3950
Position (kb)
4696
4697 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 4695.864 kb on + strand, within Psyr_3947 at 4695.865 kb on - strand, within Psyr_3947 at 4695.917 kb on - strand, within Psyr_3947 at 4696.155 kb on + strand at 4696.249 kb on - strand at 4696.249 kb on - strand at 4697.031 kb on - strand at 4697.038 kb on - strand at 4697.078 kb on - strand at 4697.179 kb on + strand, within Psyr_3949 at 4697.217 kb on - strand, within Psyr_3949 at 4697.217 kb on - strand, within Psyr_3949 at 4697.217 kb on - strand, within Psyr_3949 at 4697.252 kb on + strand, within Psyr_3949 at 4697.252 kb on + strand, within Psyr_3949 at 4697.252 kb on + strand, within Psyr_3949 at 4697.252 kb on + strand, within Psyr_3949 at 4697.252 kb on + strand, within Psyr_3949 at 4697.252 kb on + strand, within Psyr_3949 at 4697.253 kb on - strand, within Psyr_3949 at 4697.253 kb on - strand, within Psyr_3949 at 4697.399 kb on + strand, within Psyr_3949 at 4697.400 kb on - strand, within Psyr_3949 at 4697.400 kb on - strand, within Psyr_3949 at 4697.465 kb on + strand, within Psyr_3949 at 4697.465 kb on + strand, within Psyr_3949 at 4697.465 kb on + strand, within Psyr_3949 at 4697.466 kb on - strand, within Psyr_3949 at 4697.702 kb on + strand at 4697.702 kb on + strand at 4697.702 kb on + strand at 4697.702 kb on + strand at 4697.702 kb on + strand at 4697.702 kb on + strand at 4697.702 kb on + strand at 4697.703 kb on - strand at 4697.703 kb on - strand at 4697.703 kb on - strand at 4697.703 kb on - strand at 4697.703 kb on - strand at 4697.792 kb on + strand at 4697.792 kb on + strand at 4697.792 kb on + strand at 4697.793 kb on - strand at 4697.793 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Nitrofurantoin 0.0625 mg/ml remove 4,695,864 + Psyr_3947 0.61 +0.7 4,695,865 - Psyr_3947 0.61 -2.1 4,695,917 - Psyr_3947 0.66 +1.3 4,696,155 + -0.3 4,696,249 - +0.0 4,696,249 - +1.2 4,697,031 - +0.7 4,697,038 - -0.2 4,697,078 - -2.1 4,697,179 + Psyr_3949 0.19 -2.7 4,697,217 - Psyr_3949 0.25 -2.5 4,697,217 - Psyr_3949 0.25 -3.5 4,697,217 - Psyr_3949 0.25 -2.9 4,697,252 + Psyr_3949 0.30 -2.5 4,697,252 + Psyr_3949 0.30 -2.5 4,697,252 + Psyr_3949 0.30 -2.7 4,697,252 + Psyr_3949 0.30 -2.3 4,697,252 + Psyr_3949 0.30 -3.1 4,697,252 + Psyr_3949 0.30 -0.7 4,697,253 - Psyr_3949 0.30 -1.5 4,697,253 - Psyr_3949 0.30 -2.2 4,697,399 + Psyr_3949 0.51 -1.9 4,697,400 - Psyr_3949 0.52 -3.3 4,697,400 - Psyr_3949 0.52 -0.9 4,697,465 + Psyr_3949 0.61 -3.2 4,697,465 + Psyr_3949 0.61 -1.3 4,697,465 + Psyr_3949 0.61 -2.7 4,697,466 - Psyr_3949 0.61 -0.9 4,697,702 + -3.4 4,697,702 + -2.8 4,697,702 + -1.9 4,697,702 + -0.3 4,697,702 + -1.7 4,697,702 + -1.0 4,697,702 + -1.8 4,697,703 - -1.3 4,697,703 - -0.6 4,697,703 - +0.6 4,697,703 - -3.9 4,697,703 - -1.9 4,697,792 + -0.9 4,697,792 + -2.9 4,697,792 + -3.3 4,697,793 - -0.1 4,697,793 - -1.1
Or see this region's nucleotide sequence