Experiment: methylglyoxal 0.001 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_3474 and Shew_3475 are separated by 238 nucleotides Shew_3475 and Shew_3476 are separated by 156 nucleotides Shew_3476 and Shew_3477 overlap by 30 nucleotides Shew_3477 and Shew_3478 are separated by 186 nucleotides
Shew_3474: Shew_3474 - ATP-dependent DNA helicase RecQ (RefSeq), at 4,160,255 to 4,162,087
_3474
Shew_3475: Shew_3475 - hypothetical protein (RefSeq), at 4,162,326 to 4,162,790
_3475
Shew_3476: Shew_3476 - transporter DMT superfamily protein (RefSeq), at 4,162,947 to 4,163,834
_3476
Shew_3477: Shew_3477 - hypothetical protein (RefSeq), at 4,163,805 to 4,164,344
_3477
Shew_3478: Shew_3478 - N-acetylglutamate synthase (RefSeq), at 4,164,531 to 4,165,853
_3478
Position (kb)
4162
4163
4164 Strain fitness (log2 ratio)
-1
0
1 at 4161.971 kb on - strand at 4161.994 kb on - strand at 4161.994 kb on - strand at 4162.141 kb on + strand at 4162.307 kb on - strand at 4162.312 kb on - strand at 4162.353 kb on - strand at 4162.632 kb on - strand, within Shew_3475 at 4162.848 kb on + strand at 4162.856 kb on - strand at 4162.921 kb on + strand at 4163.023 kb on + strand at 4163.084 kb on - strand, within Shew_3476 at 4163.170 kb on - strand, within Shew_3476 at 4163.296 kb on + strand, within Shew_3476 at 4163.755 kb on - strand at 4163.960 kb on + strand, within Shew_3477 at 4164.097 kb on - strand, within Shew_3477 at 4164.494 kb on + strand at 4164.494 kb on + strand at 4164.502 kb on - strand at 4164.502 kb on - strand at 4164.779 kb on - strand, within Shew_3478 at 4164.809 kb on - strand, within Shew_3478 at 4164.810 kb on - strand, within Shew_3478
Per-strain Table
Position Strand Gene LocusTag Fraction methylglyoxal 0.001 vol% remove 4,161,971 - -0.9 4,161,994 - +0.2 4,161,994 - -0.3 4,162,141 + -0.1 4,162,307 - -0.0 4,162,312 - -1.0 4,162,353 - -0.4 4,162,632 - Shew_3475 0.66 -0.3 4,162,848 + +0.5 4,162,856 - +0.6 4,162,921 + +0.8 4,163,023 + +0.2 4,163,084 - Shew_3476 0.15 +0.6 4,163,170 - Shew_3476 0.25 +0.5 4,163,296 + Shew_3476 0.39 +0.9 4,163,755 - -0.1 4,163,960 + Shew_3477 0.29 +0.5 4,164,097 - Shew_3477 0.54 +0.5 4,164,494 + +0.1 4,164,494 + +1.1 4,164,502 - -0.2 4,164,502 - +0.3 4,164,779 - Shew_3478 0.19 +0.3 4,164,809 - Shew_3478 0.21 -0.2 4,164,810 - Shew_3478 0.21 -0.0
Or see this region's nucleotide sequence