Experiment: Choline Lysine 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0408 and Shew_0409 overlap by 77 nucleotides Shew_0409 and Shew_0410 are separated by 41 nucleotides Shew_0410 and Shew_0411 are separated by 192 nucleotides
Shew_0408: Shew_0408 - hypothetical protein (RefSeq), at 457,734 to 462,044
_0408
Shew_0409: Shew_0409 - Nitrilase/cyanide hydratase and apolipoprotein N-acyltransferase (RefSeq), at 461,968 to 462,798
_0409
Shew_0410: Shew_0410 - peptidase U62, modulator of DNA gyrase (RefSeq), at 462,840 to 464,288
_0410
Shew_0411: Shew_0411 - outer membrane efflux protein (RefSeq), at 464,481 to 465,884
_0411
Position (kb) 462
463
464
465 Strain fitness (log2 ratio) -1
0
1
2 at 462.097 kb on - strand, within Shew_0409 at 462.391 kb on - strand, within Shew_0409 at 462.431 kb on + strand, within Shew_0409 at 462.446 kb on - strand, within Shew_0409 at 462.615 kb on + strand, within Shew_0409 at 462.717 kb on + strand at 463.192 kb on + strand, within Shew_0410 at 463.287 kb on - strand, within Shew_0410 at 463.869 kb on + strand, within Shew_0410 at 463.882 kb on - strand, within Shew_0410 at 463.940 kb on - strand, within Shew_0410 at 464.291 kb on - strand at 464.626 kb on - strand, within Shew_0411 at 464.665 kb on - strand, within Shew_0411 at 464.937 kb on + strand, within Shew_0411 at 465.036 kb on - strand, within Shew_0411 at 465.243 kb on - strand, within Shew_0411 at 465.268 kb on + strand, within Shew_0411 at 465.276 kb on - strand, within Shew_0411
Per-strain Table
Position Strand Gene LocusTag Fraction Choline Lysine 10 mM remove 462,097 - Shew_0409 0.16 -0.2 462,391 - Shew_0409 0.51 +0.3 462,431 + Shew_0409 0.56 +0.5 462,446 - Shew_0409 0.58 +0.9 462,615 + Shew_0409 0.78 +0.2 462,717 + +0.2 463,192 + Shew_0410 0.24 +0.9 463,287 - Shew_0410 0.31 +1.5 463,869 + Shew_0410 0.71 +0.3 463,882 - Shew_0410 0.72 +1.1 463,940 - Shew_0410 0.76 +0.4 464,291 - +0.7 464,626 - Shew_0411 0.10 +0.4 464,665 - Shew_0411 0.13 +0.6 464,937 + Shew_0411 0.32 -0.4 465,036 - Shew_0411 0.40 +1.0 465,243 - Shew_0411 0.54 -0.5 465,268 + Shew_0411 0.56 +2.2 465,276 - Shew_0411 0.57 +0.3
Or see this region's nucleotide sequence