Experiment: H2 N-free with 10mM D-glucosamine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS04750 and MMJJ_RS04755 are separated by 59 nucleotides MMJJ_RS04755 and MMJJ_RS04760 are separated by 28 nucleotides
MMJJ_RS04750: MMJJ_RS04750 - 5,10-methenyltetrahydromethanopterin hydrogenase, at 890,930 to 891,994
_RS04750
MMJJ_RS04755: MMJJ_RS04755 - 5,10-methenyltetrahydromethanopterin hydrogenase cofactor biosynthesis protein HmdB, at 892,054 to 893,079
_RS04755
MMJJ_RS04760: MMJJ_RS04760 - 5,10-methenyltetrahydromethanopterin hydrogenase cofactor biosynthesis protein HmdC, at 893,108 to 894,640
_RS04760
Position (kb)
892
893
894 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 891.087 kb on - strand, within MMJJ_RS04750 at 891.087 kb on - strand, within MMJJ_RS04750 at 891.087 kb on - strand, within MMJJ_RS04750 at 891.087 kb on - strand, within MMJJ_RS04750 at 891.088 kb on + strand, within MMJJ_RS04750 at 891.100 kb on - strand, within MMJJ_RS04750 at 891.151 kb on + strand, within MMJJ_RS04750 at 891.153 kb on - strand, within MMJJ_RS04750 at 891.154 kb on + strand, within MMJJ_RS04750 at 891.182 kb on + strand, within MMJJ_RS04750 at 891.196 kb on + strand, within MMJJ_RS04750 at 891.241 kb on + strand, within MMJJ_RS04750 at 891.253 kb on - strand, within MMJJ_RS04750 at 891.295 kb on + strand, within MMJJ_RS04750 at 891.295 kb on + strand, within MMJJ_RS04750 at 891.364 kb on + strand, within MMJJ_RS04750 at 891.367 kb on - strand, within MMJJ_RS04750 at 891.367 kb on - strand, within MMJJ_RS04750 at 891.368 kb on + strand, within MMJJ_RS04750 at 891.368 kb on + strand, within MMJJ_RS04750 at 891.402 kb on - strand, within MMJJ_RS04750 at 891.445 kb on + strand, within MMJJ_RS04750 at 891.458 kb on + strand, within MMJJ_RS04750 at 891.514 kb on + strand, within MMJJ_RS04750 at 891.527 kb on - strand, within MMJJ_RS04750 at 891.583 kb on + strand, within MMJJ_RS04750 at 891.611 kb on - strand, within MMJJ_RS04750 at 891.611 kb on - strand, within MMJJ_RS04750 at 891.616 kb on - strand, within MMJJ_RS04750 at 891.663 kb on - strand, within MMJJ_RS04750 at 891.697 kb on - strand, within MMJJ_RS04750 at 891.754 kb on + strand, within MMJJ_RS04750 at 891.862 kb on - strand, within MMJJ_RS04750 at 891.913 kb on - strand at 891.914 kb on + strand at 891.914 kb on + strand at 891.992 kb on - strand at 891.997 kb on + strand at 891.997 kb on + strand at 892.000 kb on + strand at 892.002 kb on - strand at 892.003 kb on + strand at 892.009 kb on - strand at 892.010 kb on + strand at 892.038 kb on + strand at 892.059 kb on + strand at 892.070 kb on - strand at 892.107 kb on + strand at 892.112 kb on - strand at 892.112 kb on - strand at 892.125 kb on + strand at 892.152 kb on + strand at 892.155 kb on - strand at 892.156 kb on + strand at 892.181 kb on - strand, within MMJJ_RS04755 at 892.182 kb on + strand, within MMJJ_RS04755 at 892.210 kb on + strand at 892.210 kb on + strand, within MMJJ_RS04755 at 892.256 kb on - strand, within MMJJ_RS04755 at 892.256 kb on - strand, within MMJJ_RS04755 at 892.260 kb on - strand, within MMJJ_RS04755 at 892.260 kb on - strand, within MMJJ_RS04755 at 892.260 kb on - strand, within MMJJ_RS04755 at 892.260 kb on - strand, within MMJJ_RS04755 at 892.261 kb on + strand, within MMJJ_RS04755 at 892.261 kb on + strand, within MMJJ_RS04755 at 892.261 kb on + strand, within MMJJ_RS04755 at 892.264 kb on + strand, within MMJJ_RS04755 at 892.291 kb on - strand, within MMJJ_RS04755 at 892.292 kb on + strand, within MMJJ_RS04755 at 892.328 kb on + strand, within MMJJ_RS04755 at 892.331 kb on - strand, within MMJJ_RS04755 at 892.331 kb on - strand, within MMJJ_RS04755 at 892.332 kb on + strand, within MMJJ_RS04755 at 892.332 kb on + strand, within MMJJ_RS04755 at 892.445 kb on - strand, within MMJJ_RS04755 at 892.457 kb on - strand, within MMJJ_RS04755 at 892.484 kb on - strand, within MMJJ_RS04755 at 892.514 kb on - strand, within MMJJ_RS04755 at 892.546 kb on - strand, within MMJJ_RS04755 at 892.548 kb on - strand, within MMJJ_RS04755 at 892.553 kb on - strand, within MMJJ_RS04755 at 892.553 kb on - strand, within MMJJ_RS04755 at 892.553 kb on - strand at 892.557 kb on - strand, within MMJJ_RS04755 at 892.558 kb on + strand, within MMJJ_RS04755 at 892.558 kb on + strand, within MMJJ_RS04755 at 892.563 kb on + strand, within MMJJ_RS04755 at 892.637 kb on - strand, within MMJJ_RS04755 at 892.671 kb on + strand, within MMJJ_RS04755 at 892.676 kb on - strand, within MMJJ_RS04755 at 892.694 kb on - strand, within MMJJ_RS04755 at 892.699 kb on + strand, within MMJJ_RS04755 at 892.719 kb on - strand, within MMJJ_RS04755 at 892.720 kb on + strand, within MMJJ_RS04755 at 892.773 kb on + strand, within MMJJ_RS04755 at 892.790 kb on + strand, within MMJJ_RS04755 at 892.860 kb on + strand, within MMJJ_RS04755 at 892.888 kb on + strand, within MMJJ_RS04755 at 892.964 kb on - strand, within MMJJ_RS04755 at 892.992 kb on - strand at 893.036 kb on - strand at 893.037 kb on + strand at 893.037 kb on + strand at 893.045 kb on - strand at 893.073 kb on + strand at 893.077 kb on - strand at 893.077 kb on - strand at 893.078 kb on + strand at 893.087 kb on + strand at 893.096 kb on - strand at 893.142 kb on - strand at 893.401 kb on + strand, within MMJJ_RS04760 at 893.401 kb on + strand, within MMJJ_RS04760 at 893.478 kb on + strand, within MMJJ_RS04760 at 893.479 kb on - strand, within MMJJ_RS04760 at 893.482 kb on - strand, within MMJJ_RS04760 at 893.653 kb on - strand, within MMJJ_RS04760 at 893.811 kb on - strand, within MMJJ_RS04760 at 893.811 kb on - strand, within MMJJ_RS04760 at 893.811 kb on - strand, within MMJJ_RS04760 at 893.874 kb on - strand, within MMJJ_RS04760 at 893.874 kb on - strand, within MMJJ_RS04760 at 893.874 kb on - strand, within MMJJ_RS04760 at 893.875 kb on + strand, within MMJJ_RS04760 at 893.875 kb on + strand, within MMJJ_RS04760 at 893.875 kb on + strand, within MMJJ_RS04760 at 893.875 kb on + strand, within MMJJ_RS04760 at 893.875 kb on + strand, within MMJJ_RS04760 at 893.880 kb on - strand, within MMJJ_RS04760 at 893.881 kb on + strand, within MMJJ_RS04760 at 893.899 kb on - strand, within MMJJ_RS04760 at 894.037 kb on + strand, within MMJJ_RS04760 at 894.037 kb on + strand, within MMJJ_RS04760 at 894.073 kb on - strand, within MMJJ_RS04760 at 894.073 kb on - strand, within MMJJ_RS04760 at 894.073 kb on - strand, within MMJJ_RS04760 at 894.073 kb on - strand, within MMJJ_RS04760 at 894.073 kb on - strand, within MMJJ_RS04760 at 894.073 kb on - strand, within MMJJ_RS04760 at 894.074 kb on + strand, within MMJJ_RS04760
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM D-glucosamine remove 891,087 - MMJJ_RS04750 0.15 -2.6 891,087 - MMJJ_RS04750 0.15 -1.8 891,087 - MMJJ_RS04750 0.15 +1.0 891,087 - MMJJ_RS04750 0.15 -1.8 891,088 + MMJJ_RS04750 0.15 +0.1 891,100 - MMJJ_RS04750 0.16 -1.3 891,151 + MMJJ_RS04750 0.21 -2.3 891,153 - MMJJ_RS04750 0.21 -2.9 891,154 + MMJJ_RS04750 0.21 -2.2 891,182 + MMJJ_RS04750 0.24 -2.8 891,196 + MMJJ_RS04750 0.25 -0.6 891,241 + MMJJ_RS04750 0.29 -2.6 891,253 - MMJJ_RS04750 0.30 +0.2 891,295 + MMJJ_RS04750 0.34 -3.1 891,295 + MMJJ_RS04750 0.34 -1.7 891,364 + MMJJ_RS04750 0.41 -0.4 891,367 - MMJJ_RS04750 0.41 -1.7 891,367 - MMJJ_RS04750 0.41 +0.6 891,368 + MMJJ_RS04750 0.41 -1.8 891,368 + MMJJ_RS04750 0.41 -0.3 891,402 - MMJJ_RS04750 0.44 -0.5 891,445 + MMJJ_RS04750 0.48 -0.7 891,458 + MMJJ_RS04750 0.50 -1.1 891,514 + MMJJ_RS04750 0.55 +3.8 891,527 - MMJJ_RS04750 0.56 +1.8 891,583 + MMJJ_RS04750 0.61 -1.1 891,611 - MMJJ_RS04750 0.64 -0.6 891,611 - MMJJ_RS04750 0.64 -2.8 891,616 - MMJJ_RS04750 0.64 -1.4 891,663 - MMJJ_RS04750 0.69 +0.3 891,697 - MMJJ_RS04750 0.72 -0.4 891,754 + MMJJ_RS04750 0.77 -0.6 891,862 - MMJJ_RS04750 0.88 -0.3 891,913 - -1.1 891,914 + -1.7 891,914 + -2.6 891,992 - +0.9 891,997 + -1.5 891,997 + -0.6 892,000 + -1.0 892,002 - -1.8 892,003 + -0.5 892,009 - -2.1 892,010 + -0.6 892,038 + -0.9 892,059 + -3.2 892,070 - -1.5 892,107 + -0.5 892,112 - -2.4 892,112 - -0.1 892,125 + -1.9 892,152 + -1.4 892,155 - -1.1 892,156 + -1.2 892,181 - MMJJ_RS04755 0.12 -1.5 892,182 + MMJJ_RS04755 0.12 -3.4 892,210 + -0.1 892,210 + MMJJ_RS04755 0.15 -3.2 892,256 - MMJJ_RS04755 0.20 -2.2 892,256 - MMJJ_RS04755 0.20 -0.8 892,260 - MMJJ_RS04755 0.20 -0.6 892,260 - MMJJ_RS04755 0.20 -2.9 892,260 - MMJJ_RS04755 0.20 -1.7 892,260 - MMJJ_RS04755 0.20 -2.4 892,261 + MMJJ_RS04755 0.20 -0.7 892,261 + MMJJ_RS04755 0.20 +0.1 892,261 + MMJJ_RS04755 0.20 -0.8 892,264 + MMJJ_RS04755 0.20 -1.4 892,291 - MMJJ_RS04755 0.23 -2.1 892,292 + MMJJ_RS04755 0.23 -3.4 892,328 + MMJJ_RS04755 0.27 -2.9 892,331 - MMJJ_RS04755 0.27 -2.4 892,331 - MMJJ_RS04755 0.27 -1.7 892,332 + MMJJ_RS04755 0.27 -2.5 892,332 + MMJJ_RS04755 0.27 -1.4 892,445 - MMJJ_RS04755 0.38 -2.9 892,457 - MMJJ_RS04755 0.39 -2.6 892,484 - MMJJ_RS04755 0.42 -1.8 892,514 - MMJJ_RS04755 0.45 -1.8 892,546 - MMJJ_RS04755 0.48 -0.3 892,548 - MMJJ_RS04755 0.48 -1.2 892,553 - MMJJ_RS04755 0.49 -1.8 892,553 - MMJJ_RS04755 0.49 -2.3 892,553 - -0.9 892,557 - MMJJ_RS04755 0.49 -1.0 892,558 + MMJJ_RS04755 0.49 -1.7 892,558 + MMJJ_RS04755 0.49 -0.8 892,563 + MMJJ_RS04755 0.50 -0.1 892,637 - MMJJ_RS04755 0.57 +2.2 892,671 + MMJJ_RS04755 0.60 -0.2 892,676 - MMJJ_RS04755 0.61 -2.2 892,694 - MMJJ_RS04755 0.62 -1.0 892,699 + MMJJ_RS04755 0.63 -4.0 892,719 - MMJJ_RS04755 0.65 -2.9 892,720 + MMJJ_RS04755 0.65 -3.7 892,773 + MMJJ_RS04755 0.70 -1.2 892,790 + MMJJ_RS04755 0.72 -0.8 892,860 + MMJJ_RS04755 0.79 -0.4 892,888 + MMJJ_RS04755 0.81 -2.6 892,964 - MMJJ_RS04755 0.89 -1.8 892,992 - -0.5 893,036 - -0.7 893,037 + -2.4 893,037 + -2.0 893,045 - -2.6 893,073 + -0.6 893,077 - -1.0 893,077 - +0.7 893,078 + -0.5 893,087 + -0.7 893,096 - +0.6 893,142 - -2.3 893,401 + MMJJ_RS04760 0.19 -0.9 893,401 + MMJJ_RS04760 0.19 +0.4 893,478 + MMJJ_RS04760 0.24 -2.7 893,479 - MMJJ_RS04760 0.24 -2.6 893,482 - MMJJ_RS04760 0.24 -2.3 893,653 - MMJJ_RS04760 0.36 -1.7 893,811 - MMJJ_RS04760 0.46 -1.6 893,811 - MMJJ_RS04760 0.46 -0.7 893,811 - MMJJ_RS04760 0.46 -1.0 893,874 - MMJJ_RS04760 0.50 -1.2 893,874 - MMJJ_RS04760 0.50 -2.2 893,874 - MMJJ_RS04760 0.50 -1.8 893,875 + MMJJ_RS04760 0.50 -3.6 893,875 + MMJJ_RS04760 0.50 -2.9 893,875 + MMJJ_RS04760 0.50 -2.2 893,875 + MMJJ_RS04760 0.50 -1.4 893,875 + MMJJ_RS04760 0.50 -1.4 893,880 - MMJJ_RS04760 0.50 -1.4 893,881 + MMJJ_RS04760 0.50 -3.1 893,899 - MMJJ_RS04760 0.52 -2.3 894,037 + MMJJ_RS04760 0.61 -1.4 894,037 + MMJJ_RS04760 0.61 -2.1 894,073 - MMJJ_RS04760 0.63 -1.9 894,073 - MMJJ_RS04760 0.63 -2.8 894,073 - MMJJ_RS04760 0.63 -2.7 894,073 - MMJJ_RS04760 0.63 -2.8 894,073 - MMJJ_RS04760 0.63 -1.4 894,073 - MMJJ_RS04760 0.63 -1.1 894,074 + MMJJ_RS04760 0.63 -3.2
Or see this region's nucleotide sequence