Experiment: KB with capsaicin 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1841 and Psyr_1842 are separated by 39 nucleotides Psyr_1842 and Psyr_1843 are separated by 30 nucleotides Psyr_1843 and Psyr_1844 are separated by 21 nucleotides Psyr_1844 and Psyr_1845 are separated by 85 nucleotides
Psyr_1841: Psyr_1841 - Aminotransferase, class I and II, at 2,121,278 to 2,122,444
_1841
Psyr_1842: Psyr_1842 - Glutathione S-transferase, N-terminal:Glutathione S-transferase, C-terminal, at 2,122,484 to 2,123,086
_1842
Psyr_1843: Psyr_1843 - Protein of unknown function DUF1311, at 2,123,117 to 2,123,503
_1843
Psyr_1844: Psyr_1844 - conserved hypothetical protein, at 2,123,525 to 2,123,890
_1844
Psyr_1845: Psyr_1845 - GTP cyclohydrolase I, at 2,123,976 to 2,124,521
_1845
Position (kb)
2123
2124 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 2122.390 kb on + strand at 2122.392 kb on + strand at 2122.393 kb on - strand at 2122.393 kb on - strand at 2122.460 kb on + strand at 2122.460 kb on + strand at 2122.460 kb on + strand at 2122.460 kb on + strand at 2122.460 kb on + strand at 2122.461 kb on - strand at 2122.461 kb on - strand at 2122.461 kb on - strand at 2122.487 kb on + strand at 2122.488 kb on - strand at 2122.496 kb on + strand at 2122.496 kb on + strand at 2122.496 kb on + strand at 2122.506 kb on - strand at 2122.523 kb on + strand at 2122.523 kb on + strand at 2122.524 kb on - strand at 2122.524 kb on - strand at 2122.543 kb on + strand at 2122.722 kb on - strand, within Psyr_1842 at 2122.781 kb on + strand, within Psyr_1842 at 2122.781 kb on + strand, within Psyr_1842 at 2122.781 kb on + strand, within Psyr_1842 at 2122.782 kb on - strand, within Psyr_1842 at 2122.826 kb on + strand, within Psyr_1842 at 2122.826 kb on + strand, within Psyr_1842 at 2122.921 kb on + strand, within Psyr_1842 at 2122.970 kb on + strand, within Psyr_1842 at 2122.970 kb on + strand, within Psyr_1842 at 2122.970 kb on + strand, within Psyr_1842 at 2122.970 kb on + strand, within Psyr_1842 at 2122.970 kb on + strand, within Psyr_1842 at 2122.970 kb on + strand, within Psyr_1842 at 2122.970 kb on + strand, within Psyr_1842 at 2122.970 kb on + strand, within Psyr_1842 at 2122.970 kb on + strand, within Psyr_1842 at 2122.971 kb on - strand, within Psyr_1842 at 2122.971 kb on - strand, within Psyr_1842 at 2122.971 kb on - strand, within Psyr_1842 at 2122.971 kb on - strand, within Psyr_1842 at 2122.971 kb on - strand, within Psyr_1842 at 2122.971 kb on - strand, within Psyr_1842 at 2122.971 kb on - strand, within Psyr_1842 at 2122.971 kb on - strand, within Psyr_1842 at 2122.971 kb on - strand, within Psyr_1842 at 2122.971 kb on - strand, within Psyr_1842 at 2122.971 kb on - strand, within Psyr_1842 at 2122.976 kb on + strand, within Psyr_1842 at 2122.977 kb on - strand, within Psyr_1842 at 2122.977 kb on - strand, within Psyr_1842 at 2123.067 kb on - strand at 2123.068 kb on + strand at 2123.068 kb on + strand at 2123.069 kb on - strand at 2123.069 kb on - strand at 2123.069 kb on - strand at 2123.074 kb on + strand at 2123.116 kb on + strand at 2123.116 kb on + strand at 2123.116 kb on + strand at 2123.116 kb on + strand at 2123.134 kb on - strand at 2123.267 kb on + strand, within Psyr_1843 at 2123.267 kb on + strand, within Psyr_1843 at 2123.398 kb on + strand, within Psyr_1843 at 2123.468 kb on + strand at 2123.468 kb on + strand at 2123.468 kb on + strand at 2123.468 kb on + strand at 2123.468 kb on + strand at 2123.468 kb on + strand at 2123.469 kb on - strand at 2123.469 kb on - strand at 2123.469 kb on - strand at 2123.469 kb on - strand at 2123.469 kb on - strand at 2123.553 kb on + strand at 2123.577 kb on + strand, within Psyr_1844 at 2123.577 kb on + strand, within Psyr_1844 at 2123.578 kb on - strand, within Psyr_1844 at 2123.578 kb on - strand, within Psyr_1844 at 2123.578 kb on - strand, within Psyr_1844 at 2123.578 kb on - strand, within Psyr_1844 at 2123.578 kb on - strand, within Psyr_1844 at 2123.578 kb on - strand, within Psyr_1844 at 2123.578 kb on - strand, within Psyr_1844 at 2123.902 kb on + strand at 2123.903 kb on - strand at 2124.202 kb on + strand, within Psyr_1845 at 2124.202 kb on + strand, within Psyr_1845 at 2124.202 kb on + strand, within Psyr_1845 at 2124.202 kb on + strand, within Psyr_1845 at 2124.202 kb on + strand, within Psyr_1845 at 2124.202 kb on + strand, within Psyr_1845 at 2124.202 kb on + strand, within Psyr_1845 at 2124.202 kb on + strand, within Psyr_1845 at 2124.202 kb on + strand, within Psyr_1845 at 2124.202 kb on + strand, within Psyr_1845 at 2124.203 kb on - strand, within Psyr_1845 at 2124.203 kb on - strand, within Psyr_1845 at 2124.203 kb on - strand, within Psyr_1845 at 2124.203 kb on - strand, within Psyr_1845 at 2124.203 kb on - strand, within Psyr_1845 at 2124.203 kb on - strand, within Psyr_1845 at 2124.203 kb on - strand, within Psyr_1845 at 2124.203 kb on - strand, within Psyr_1845 at 2124.260 kb on - strand, within Psyr_1845 at 2124.260 kb on - strand, within Psyr_1845 at 2124.415 kb on + strand, within Psyr_1845 at 2124.415 kb on + strand, within Psyr_1845 at 2124.416 kb on - strand, within Psyr_1845 at 2124.416 kb on - strand, within Psyr_1845 at 2124.502 kb on + strand at 2124.502 kb on + strand at 2124.502 kb on + strand at 2124.502 kb on + strand at 2124.502 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with capsaicin 0.25 mg/ml remove 2,122,390 + -0.5 2,122,392 + +0.6 2,122,393 - +0.4 2,122,393 - +0.1 2,122,460 + -0.8 2,122,460 + +2.8 2,122,460 + +0.9 2,122,460 + -1.6 2,122,460 + -0.3 2,122,461 - -0.0 2,122,461 - +0.0 2,122,461 - -0.1 2,122,487 + -1.2 2,122,488 - +0.3 2,122,496 + +1.5 2,122,496 + +4.1 2,122,496 + -0.5 2,122,506 - +1.2 2,122,523 + -0.5 2,122,523 + +1.1 2,122,524 - -0.8 2,122,524 - +1.5 2,122,543 + +0.0 2,122,722 - Psyr_1842 0.39 +0.1 2,122,781 + Psyr_1842 0.49 -0.3 2,122,781 + Psyr_1842 0.49 +0.7 2,122,781 + Psyr_1842 0.49 +0.2 2,122,782 - Psyr_1842 0.49 -1.1 2,122,826 + Psyr_1842 0.57 -0.4 2,122,826 + Psyr_1842 0.57 -1.2 2,122,921 + Psyr_1842 0.72 -0.9 2,122,970 + Psyr_1842 0.81 -0.6 2,122,970 + Psyr_1842 0.81 -0.3 2,122,970 + Psyr_1842 0.81 +0.2 2,122,970 + Psyr_1842 0.81 -0.1 2,122,970 + Psyr_1842 0.81 +0.2 2,122,970 + Psyr_1842 0.81 +2.3 2,122,970 + Psyr_1842 0.81 -1.4 2,122,970 + Psyr_1842 0.81 +0.2 2,122,970 + Psyr_1842 0.81 +0.1 2,122,971 - Psyr_1842 0.81 +1.0 2,122,971 - Psyr_1842 0.81 +0.2 2,122,971 - Psyr_1842 0.81 -0.3 2,122,971 - Psyr_1842 0.81 +0.4 2,122,971 - Psyr_1842 0.81 +1.1 2,122,971 - Psyr_1842 0.81 +0.6 2,122,971 - Psyr_1842 0.81 +2.2 2,122,971 - Psyr_1842 0.81 +0.3 2,122,971 - Psyr_1842 0.81 +0.9 2,122,971 - Psyr_1842 0.81 -0.8 2,122,971 - Psyr_1842 0.81 +0.9 2,122,976 + Psyr_1842 0.82 +0.0 2,122,977 - Psyr_1842 0.82 +0.6 2,122,977 - Psyr_1842 0.82 -0.0 2,123,067 - -1.1 2,123,068 + -0.5 2,123,068 + +0.7 2,123,069 - -1.8 2,123,069 - -0.5 2,123,069 - +0.1 2,123,074 + +0.2 2,123,116 + -1.3 2,123,116 + -0.1 2,123,116 + -0.5 2,123,116 + +2.9 2,123,134 - +1.2 2,123,267 + Psyr_1843 0.39 +0.3 2,123,267 + Psyr_1843 0.39 -1.0 2,123,398 + Psyr_1843 0.73 -0.7 2,123,468 + -1.4 2,123,468 + +0.7 2,123,468 + +1.0 2,123,468 + -0.2 2,123,468 + +0.6 2,123,468 + +1.2 2,123,469 - -0.1 2,123,469 - -0.9 2,123,469 - -0.4 2,123,469 - -0.7 2,123,469 - -0.2 2,123,553 + +1.6 2,123,577 + Psyr_1844 0.14 +0.9 2,123,577 + Psyr_1844 0.14 +0.7 2,123,578 - Psyr_1844 0.14 +0.4 2,123,578 - Psyr_1844 0.14 -0.1 2,123,578 - Psyr_1844 0.14 +0.1 2,123,578 - Psyr_1844 0.14 +2.2 2,123,578 - Psyr_1844 0.14 +0.1 2,123,578 - Psyr_1844 0.14 +1.2 2,123,578 - Psyr_1844 0.14 +0.2 2,123,902 + -0.6 2,123,903 - +1.7 2,124,202 + Psyr_1845 0.41 +0.0 2,124,202 + Psyr_1845 0.41 +1.0 2,124,202 + Psyr_1845 0.41 -0.4 2,124,202 + Psyr_1845 0.41 +0.8 2,124,202 + Psyr_1845 0.41 +0.0 2,124,202 + Psyr_1845 0.41 -1.1 2,124,202 + Psyr_1845 0.41 -0.2 2,124,202 + Psyr_1845 0.41 -2.4 2,124,202 + Psyr_1845 0.41 -0.3 2,124,202 + Psyr_1845 0.41 +1.0 2,124,203 - Psyr_1845 0.42 -1.6 2,124,203 - Psyr_1845 0.42 -2.0 2,124,203 - Psyr_1845 0.42 +0.3 2,124,203 - Psyr_1845 0.42 +1.0 2,124,203 - Psyr_1845 0.42 -0.5 2,124,203 - Psyr_1845 0.42 +1.1 2,124,203 - Psyr_1845 0.42 +0.6 2,124,203 - Psyr_1845 0.42 -0.5 2,124,260 - Psyr_1845 0.52 +0.6 2,124,260 - Psyr_1845 0.52 +0.4 2,124,415 + Psyr_1845 0.80 -0.4 2,124,415 + Psyr_1845 0.80 +0.1 2,124,416 - Psyr_1845 0.81 -0.6 2,124,416 - Psyr_1845 0.81 -0.5 2,124,502 + +1.2 2,124,502 + +1.6 2,124,502 + -3.8 2,124,502 + -2.1 2,124,502 + -0.1
Or see this region's nucleotide sequence