Experiment: KB with capsaicin 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0289 and Psyr_0290 are separated by 122 nucleotides Psyr_0290 and Psyr_0291 are separated by 186 nucleotides Psyr_0291 and Psyr_0292 are separated by 231 nucleotides
Psyr_0289: Psyr_0289 - Radical SAM, at 307,483 to 308,541
_0289
Psyr_0290: Psyr_0290 - ThiJ/PfpI, at 308,664 to 309,332
_0290
Psyr_0291: Psyr_0291 - DedA, at 309,519 to 310,181
_0291
Psyr_0292: Psyr_0292 - porphobilinogen synthase, at 310,413 to 311,423
_0292
Position (kb) 309
310
311 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 308.549 kb on + strand at 308.549 kb on + strand at 308.550 kb on - strand at 308.557 kb on + strand at 308.570 kb on + strand at 308.575 kb on + strand at 308.582 kb on + strand at 308.582 kb on + strand at 308.583 kb on - strand at 308.755 kb on + strand, within Psyr_0290 at 308.755 kb on + strand, within Psyr_0290 at 308.755 kb on + strand, within Psyr_0290 at 308.755 kb on + strand, within Psyr_0290 at 308.755 kb on + strand, within Psyr_0290 at 308.755 kb on + strand, within Psyr_0290 at 308.755 kb on + strand, within Psyr_0290 at 308.755 kb on + strand, within Psyr_0290 at 308.756 kb on - strand, within Psyr_0290 at 308.756 kb on - strand, within Psyr_0290 at 308.756 kb on - strand, within Psyr_0290 at 308.756 kb on - strand, within Psyr_0290 at 308.756 kb on - strand, within Psyr_0290 at 308.756 kb on - strand, within Psyr_0290 at 308.756 kb on - strand, within Psyr_0290 at 308.756 kb on - strand, within Psyr_0290 at 308.896 kb on + strand, within Psyr_0290 at 308.896 kb on + strand, within Psyr_0290 at 308.896 kb on + strand, within Psyr_0290 at 308.897 kb on - strand, within Psyr_0290 at 309.290 kb on - strand at 309.312 kb on + strand at 309.312 kb on + strand at 309.313 kb on - strand at 309.374 kb on + strand at 309.375 kb on - strand at 309.521 kb on - strand at 309.709 kb on + strand, within Psyr_0291 at 309.710 kb on - strand, within Psyr_0291 at 309.758 kb on - strand, within Psyr_0291 at 309.817 kb on + strand, within Psyr_0291 at 309.817 kb on + strand, within Psyr_0291 at 310.007 kb on - strand, within Psyr_0291 at 310.007 kb on - strand, within Psyr_0291 at 310.007 kb on - strand, within Psyr_0291 at 310.027 kb on + strand, within Psyr_0291 at 310.027 kb on + strand, within Psyr_0291 at 310.027 kb on + strand, within Psyr_0291 at 310.028 kb on - strand, within Psyr_0291 at 310.028 kb on - strand, within Psyr_0291 at 310.044 kb on + strand, within Psyr_0291 at 310.073 kb on - strand, within Psyr_0291 at 310.073 kb on - strand, within Psyr_0291 at 310.246 kb on + strand at 310.246 kb on + strand at 310.246 kb on + strand at 310.246 kb on + strand at 310.246 kb on + strand at 310.246 kb on + strand at 310.246 kb on + strand at 310.246 kb on + strand at 310.246 kb on + strand at 310.246 kb on + strand at 310.246 kb on + strand at 310.246 kb on + strand at 310.246 kb on + strand at 310.246 kb on + strand at 310.247 kb on - strand at 310.247 kb on - strand at 310.247 kb on - strand at 310.247 kb on - strand at 310.247 kb on - strand at 310.247 kb on - strand at 310.247 kb on - strand at 310.247 kb on - strand at 310.247 kb on - strand at 310.247 kb on - strand at 310.247 kb on - strand at 310.247 kb on - strand at 310.247 kb on - strand at 310.289 kb on - strand at 310.289 kb on - strand at 310.289 kb on - strand at 310.367 kb on + strand at 310.367 kb on + strand at 310.369 kb on + strand at 310.369 kb on + strand at 310.369 kb on + strand at 310.369 kb on + strand at 310.369 kb on + strand at 310.369 kb on + strand at 310.369 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with capsaicin 0.25 mg/ml remove 308,549 + +0.4 308,549 + +1.4 308,550 - +0.3 308,557 + +1.2 308,570 + -0.8 308,575 + -0.8 308,582 + -0.4 308,582 + +0.6 308,583 - -0.4 308,755 + Psyr_0290 0.14 -0.1 308,755 + Psyr_0290 0.14 +0.1 308,755 + Psyr_0290 0.14 +0.3 308,755 + Psyr_0290 0.14 +0.3 308,755 + Psyr_0290 0.14 +0.1 308,755 + Psyr_0290 0.14 -1.2 308,755 + Psyr_0290 0.14 +0.4 308,755 + Psyr_0290 0.14 +1.5 308,756 - Psyr_0290 0.14 +2.2 308,756 - Psyr_0290 0.14 +0.4 308,756 - Psyr_0290 0.14 +0.2 308,756 - Psyr_0290 0.14 +1.0 308,756 - Psyr_0290 0.14 -0.1 308,756 - Psyr_0290 0.14 +0.2 308,756 - Psyr_0290 0.14 +1.2 308,756 - Psyr_0290 0.14 +0.7 308,896 + Psyr_0290 0.35 -0.3 308,896 + Psyr_0290 0.35 -1.3 308,896 + Psyr_0290 0.35 -0.1 308,897 - Psyr_0290 0.35 -0.8 309,290 - +1.1 309,312 + +0.2 309,312 + -0.0 309,313 - -0.1 309,374 + -0.0 309,375 - +1.0 309,521 - +0.6 309,709 + Psyr_0291 0.29 -3.2 309,710 - Psyr_0291 0.29 -1.2 309,758 - Psyr_0291 0.36 -0.3 309,817 + Psyr_0291 0.45 -1.0 309,817 + Psyr_0291 0.45 -0.4 310,007 - Psyr_0291 0.74 -0.5 310,007 - Psyr_0291 0.74 -1.9 310,007 - Psyr_0291 0.74 -1.0 310,027 + Psyr_0291 0.77 -0.6 310,027 + Psyr_0291 0.77 +0.2 310,027 + Psyr_0291 0.77 -1.4 310,028 - Psyr_0291 0.77 -0.1 310,028 - Psyr_0291 0.77 +0.5 310,044 + Psyr_0291 0.79 -0.8 310,073 - Psyr_0291 0.84 +0.1 310,073 - Psyr_0291 0.84 +0.1 310,246 + -0.1 310,246 + -0.1 310,246 + -1.0 310,246 + +1.7 310,246 + +1.9 310,246 + -0.6 310,246 + +0.2 310,246 + +1.0 310,246 + -1.1 310,246 + +0.5 310,246 + +0.9 310,246 + -1.1 310,246 + +1.5 310,246 + -0.8 310,247 - -0.2 310,247 - +0.4 310,247 - +0.5 310,247 - +0.9 310,247 - -0.4 310,247 - +0.8 310,247 - -0.7 310,247 - -0.7 310,247 - +3.8 310,247 - +1.2 310,247 - +0.2 310,247 - -2.2 310,247 - +0.3 310,289 - +0.7 310,289 - +0.7 310,289 - -1.7 310,367 + +1.2 310,367 + -0.2 310,369 + -2.4 310,369 + +0.4 310,369 + +1.1 310,369 + -0.3 310,369 + -0.3 310,369 + -0.8 310,369 + -2.2
Or see this region's nucleotide sequence