Experiment: KB with capsaicin 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0032 and Psyr_0033 are separated by 125 nucleotides Psyr_0033 and Psyr_0034 overlap by 4 nucleotides Psyr_0034 and Psyr_0035 are separated by 79 nucleotides
Psyr_0032: Psyr_0032 - conserved hypothetical protein, at 36,536 to 37,066
_0032
Psyr_0033: Psyr_0033 - tryptophan synthase, alpha chain, at 37,192 to 38,001
_0033
Psyr_0034: Psyr_0034 - tryptophan synthase, beta chain, at 37,998 to 39,227
_0034
Psyr_0035: Psyr_0035 - regulatory protein, LysR:LysR, substrate-binding protein, at 39,307 to 40,263
_0035
Position (kb) 37
38
39
40 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 37.045 kb on + strand at 37.140 kb on + strand at 37.140 kb on + strand at 37.140 kb on + strand at 37.140 kb on + strand at 37.140 kb on + strand at 37.140 kb on + strand at 37.140 kb on + strand at 37.140 kb on + strand at 37.140 kb on + strand at 37.140 kb on + strand at 37.140 kb on + strand at 37.141 kb on - strand at 37.141 kb on - strand at 37.141 kb on - strand at 37.141 kb on - strand at 37.141 kb on - strand at 37.141 kb on - strand at 37.141 kb on - strand at 37.141 kb on - strand at 37.141 kb on - strand at 37.141 kb on - strand at 37.144 kb on + strand at 37.144 kb on + strand at 37.144 kb on + strand at 37.144 kb on + strand at 37.144 kb on + strand at 37.144 kb on + strand at 37.144 kb on + strand at 37.145 kb on - strand at 37.145 kb on - strand at 37.145 kb on - strand at 37.145 kb on - strand at 37.145 kb on - strand at 37.145 kb on - strand at 37.145 kb on - strand at 37.145 kb on - strand at 37.145 kb on - strand at 37.176 kb on - strand at 37.207 kb on - strand at 37.207 kb on - strand at 37.210 kb on + strand at 37.210 kb on + strand at 37.211 kb on - strand at 37.211 kb on - strand at 37.211 kb on - strand at 37.211 kb on - strand at 37.211 kb on - strand at 37.475 kb on + strand, within Psyr_0033 at 37.475 kb on + strand, within Psyr_0033 at 37.475 kb on + strand, within Psyr_0033 at 37.475 kb on + strand, within Psyr_0033 at 37.476 kb on - strand, within Psyr_0033 at 37.476 kb on - strand, within Psyr_0033 at 37.476 kb on - strand, within Psyr_0033 at 37.478 kb on + strand, within Psyr_0033 at 37.695 kb on - strand, within Psyr_0033 at 37.695 kb on - strand, within Psyr_0033 at 37.695 kb on - strand, within Psyr_0033 at 37.695 kb on - strand, within Psyr_0033 at 37.697 kb on + strand, within Psyr_0033 at 37.991 kb on - strand at 37.991 kb on - strand at 38.002 kb on - strand at 38.234 kb on - strand, within Psyr_0034 at 38.234 kb on - strand, within Psyr_0034 at 38.234 kb on - strand, within Psyr_0034 at 38.350 kb on + strand, within Psyr_0034 at 38.351 kb on - strand, within Psyr_0034 at 38.351 kb on - strand, within Psyr_0034 at 38.351 kb on - strand, within Psyr_0034 at 38.648 kb on - strand, within Psyr_0034 at 38.806 kb on + strand, within Psyr_0034 at 38.806 kb on + strand, within Psyr_0034 at 38.806 kb on + strand, within Psyr_0034 at 38.807 kb on - strand, within Psyr_0034 at 38.807 kb on - strand, within Psyr_0034 at 38.807 kb on - strand, within Psyr_0034 at 38.807 kb on - strand, within Psyr_0034 at 38.807 kb on - strand, within Psyr_0034 at 38.807 kb on - strand, within Psyr_0034 at 38.920 kb on - strand, within Psyr_0034 at 39.028 kb on + strand, within Psyr_0034 at 39.028 kb on + strand, within Psyr_0034 at 39.053 kb on - strand, within Psyr_0034 at 39.053 kb on - strand, within Psyr_0034 at 39.068 kb on - strand, within Psyr_0034 at 39.257 kb on + strand at 39.258 kb on - strand at 39.258 kb on - strand at 39.258 kb on - strand at 39.270 kb on - strand at 39.602 kb on + strand, within Psyr_0035 at 39.602 kb on + strand, within Psyr_0035 at 39.602 kb on + strand, within Psyr_0035 at 39.602 kb on + strand, within Psyr_0035 at 39.602 kb on + strand, within Psyr_0035 at 39.602 kb on + strand, within Psyr_0035 at 39.602 kb on + strand, within Psyr_0035 at 39.602 kb on + strand, within Psyr_0035 at 39.602 kb on + strand, within Psyr_0035 at 39.602 kb on + strand, within Psyr_0035 at 39.602 kb on + strand, within Psyr_0035 at 39.602 kb on + strand, within Psyr_0035 at 39.602 kb on + strand, within Psyr_0035 at 39.602 kb on + strand, within Psyr_0035 at 39.602 kb on + strand, within Psyr_0035 at 39.602 kb on + strand, within Psyr_0035 at 39.602 kb on + strand, within Psyr_0035 at 39.602 kb on + strand, within Psyr_0035 at 39.602 kb on + strand, within Psyr_0035 at 39.602 kb on + strand, within Psyr_0035 at 39.602 kb on + strand, within Psyr_0035 at 39.602 kb on + strand, within Psyr_0035 at 39.603 kb on - strand, within Psyr_0035 at 39.603 kb on - strand, within Psyr_0035 at 39.603 kb on - strand, within Psyr_0035 at 39.603 kb on - strand, within Psyr_0035 at 39.603 kb on - strand, within Psyr_0035 at 39.603 kb on - strand, within Psyr_0035 at 39.603 kb on - strand, within Psyr_0035 at 39.603 kb on - strand, within Psyr_0035 at 39.603 kb on - strand, within Psyr_0035 at 39.603 kb on - strand, within Psyr_0035 at 39.792 kb on - strand, within Psyr_0035 at 39.792 kb on - strand, within Psyr_0035 at 39.792 kb on - strand, within Psyr_0035 at 40.033 kb on + strand, within Psyr_0035 at 40.033 kb on + strand, within Psyr_0035 at 40.034 kb on - strand, within Psyr_0035 at 40.034 kb on - strand, within Psyr_0035 at 40.034 kb on - strand, within Psyr_0035 at 40.034 kb on - strand, within Psyr_0035 at 40.037 kb on - strand, within Psyr_0035 at 40.037 kb on - strand, within Psyr_0035 at 40.037 kb on - strand, within Psyr_0035 at 40.037 kb on - strand, within Psyr_0035 at 40.037 kb on - strand, within Psyr_0035
Per-strain Table
Position Strand Gene LocusTag Fraction KB with capsaicin 0.25 mg/ml remove 37,045 + -0.6 37,140 + -2.3 37,140 + -0.5 37,140 + -0.0 37,140 + -0.7 37,140 + -0.3 37,140 + +0.0 37,140 + -0.4 37,140 + -1.6 37,140 + -0.3 37,140 + +0.2 37,140 + +0.1 37,141 - +0.4 37,141 - -0.3 37,141 - -0.0 37,141 - -0.5 37,141 - +0.4 37,141 - -0.2 37,141 - +0.5 37,141 - -1.2 37,141 - +0.1 37,141 - +0.5 37,144 + +0.8 37,144 + -1.4 37,144 + +0.7 37,144 + +0.1 37,144 + -0.3 37,144 + +0.5 37,144 + -1.3 37,145 - -0.8 37,145 - +0.2 37,145 - +1.2 37,145 - +1.2 37,145 - -0.3 37,145 - +1.2 37,145 - +2.2 37,145 - -0.2 37,145 - -0.1 37,176 - +0.3 37,207 - -3.4 37,207 - +0.6 37,210 + -0.8 37,210 + -1.7 37,211 - +1.2 37,211 - +0.4 37,211 - +0.3 37,211 - -0.8 37,211 - +0.2 37,475 + Psyr_0033 0.35 -1.3 37,475 + Psyr_0033 0.35 -2.2 37,475 + Psyr_0033 0.35 +1.2 37,475 + Psyr_0033 0.35 -0.9 37,476 - Psyr_0033 0.35 -0.5 37,476 - Psyr_0033 0.35 +2.6 37,476 - Psyr_0033 0.35 -0.3 37,478 + Psyr_0033 0.35 +0.7 37,695 - Psyr_0033 0.62 +0.5 37,695 - Psyr_0033 0.62 +0.3 37,695 - Psyr_0033 0.62 -2.2 37,695 - Psyr_0033 0.62 +0.0 37,697 + Psyr_0033 0.62 -1.8 37,991 - +0.1 37,991 - -0.2 38,002 - +1.2 38,234 - Psyr_0034 0.19 +0.0 38,234 - Psyr_0034 0.19 -1.4 38,234 - Psyr_0034 0.19 -0.8 38,350 + Psyr_0034 0.29 -1.8 38,351 - Psyr_0034 0.29 -1.6 38,351 - Psyr_0034 0.29 +0.4 38,351 - Psyr_0034 0.29 -1.6 38,648 - Psyr_0034 0.53 -0.8 38,806 + Psyr_0034 0.66 -1.6 38,806 + Psyr_0034 0.66 -1.9 38,806 + Psyr_0034 0.66 -1.9 38,807 - Psyr_0034 0.66 -0.5 38,807 - Psyr_0034 0.66 -0.8 38,807 - Psyr_0034 0.66 -1.6 38,807 - Psyr_0034 0.66 -1.1 38,807 - Psyr_0034 0.66 +1.6 38,807 - Psyr_0034 0.66 +1.2 38,920 - Psyr_0034 0.75 +0.5 39,028 + Psyr_0034 0.84 -2.5 39,028 + Psyr_0034 0.84 -1.3 39,053 - Psyr_0034 0.86 -0.9 39,053 - Psyr_0034 0.86 -1.8 39,068 - Psyr_0034 0.87 -1.3 39,257 + -0.1 39,258 - -0.1 39,258 - -0.8 39,258 - +0.1 39,270 - -1.5 39,602 + Psyr_0035 0.31 -1.1 39,602 + Psyr_0035 0.31 +0.1 39,602 + Psyr_0035 0.31 -1.5 39,602 + Psyr_0035 0.31 +0.5 39,602 + Psyr_0035 0.31 -0.5 39,602 + Psyr_0035 0.31 -1.0 39,602 + Psyr_0035 0.31 +0.2 39,602 + Psyr_0035 0.31 -1.1 39,602 + Psyr_0035 0.31 -0.1 39,602 + Psyr_0035 0.31 +0.9 39,602 + Psyr_0035 0.31 +0.4 39,602 + Psyr_0035 0.31 -0.6 39,602 + Psyr_0035 0.31 +0.7 39,602 + Psyr_0035 0.31 -0.1 39,602 + Psyr_0035 0.31 -0.5 39,602 + Psyr_0035 0.31 -1.1 39,602 + Psyr_0035 0.31 -0.3 39,602 + Psyr_0035 0.31 +1.1 39,602 + Psyr_0035 0.31 -1.8 39,602 + Psyr_0035 0.31 -0.1 39,602 + Psyr_0035 0.31 +0.3 39,602 + Psyr_0035 0.31 -0.5 39,603 - Psyr_0035 0.31 +0.7 39,603 - Psyr_0035 0.31 -0.8 39,603 - Psyr_0035 0.31 -0.8 39,603 - Psyr_0035 0.31 -1.1 39,603 - Psyr_0035 0.31 +0.3 39,603 - Psyr_0035 0.31 +0.1 39,603 - Psyr_0035 0.31 -0.3 39,603 - Psyr_0035 0.31 +0.7 39,603 - Psyr_0035 0.31 +1.1 39,603 - Psyr_0035 0.31 -0.6 39,792 - Psyr_0035 0.51 -0.1 39,792 - Psyr_0035 0.51 -0.5 39,792 - Psyr_0035 0.51 -1.4 40,033 + Psyr_0035 0.76 +0.4 40,033 + Psyr_0035 0.76 +0.4 40,034 - Psyr_0035 0.76 +0.7 40,034 - Psyr_0035 0.76 +1.8 40,034 - Psyr_0035 0.76 +0.6 40,034 - Psyr_0035 0.76 +0.8 40,037 - Psyr_0035 0.76 -0.7 40,037 - Psyr_0035 0.76 +0.2 40,037 - Psyr_0035 0.76 -0.7 40,037 - Psyr_0035 0.76 -0.8 40,037 - Psyr_0035 0.76 -0.4
Or see this region's nucleotide sequence