Experiment: H2 N-free with 2g/L casaminos 10mM Ammonium chloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS04585 and MMJJ_RS04590 are separated by 71 nucleotides MMJJ_RS04590 and MMJJ_RS04595 are separated by 16 nucleotides MMJJ_RS04595 and MMJJ_RS04600 are separated by 0 nucleotides MMJJ_RS04600 and MMJJ_RS04605 are separated by 25 nucleotides
MMJJ_RS04585: MMJJ_RS04585 - phenylacetate--CoA ligase, at 855,178 to 856,479
_RS04585
MMJJ_RS04590: MMJJ_RS04590 - 50S ribosomal protein L39e, at 856,551 to 856,706
_RS04590
MMJJ_RS04595: MMJJ_RS04595 - hypothetical protein, at 856,723 to 857,322
_RS04595
MMJJ_RS04600: MMJJ_RS04600 - DNA-binding protein, at 857,323 to 857,682
_RS04600
MMJJ_RS04605: MMJJ_RS04605 - 30S ribosomal protein S19e, at 857,708 to 858,133
_RS04605
Position (kb) 856
857
858 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 855.794 kb on - strand, within MMJJ_RS04585 at 855.893 kb on - strand, within MMJJ_RS04585 at 855.943 kb on + strand, within MMJJ_RS04585 at 856.025 kb on - strand, within MMJJ_RS04585 at 856.025 kb on - strand, within MMJJ_RS04585 at 856.029 kb on - strand, within MMJJ_RS04585 at 856.038 kb on - strand, within MMJJ_RS04585 at 856.038 kb on - strand, within MMJJ_RS04585 at 856.039 kb on + strand, within MMJJ_RS04585 at 856.039 kb on + strand, within MMJJ_RS04585 at 856.062 kb on - strand, within MMJJ_RS04585 at 856.275 kb on + strand, within MMJJ_RS04585 at 856.294 kb on + strand, within MMJJ_RS04585 at 856.296 kb on + strand, within MMJJ_RS04585 at 856.365 kb on - strand at 856.366 kb on + strand at 856.406 kb on - strand at 856.417 kb on + strand at 856.417 kb on + strand at 856.441 kb on + strand at 856.478 kb on + strand at 856.491 kb on + strand at 856.725 kb on + strand at 856.769 kb on - strand at 856.769 kb on - strand at 856.882 kb on - strand, within MMJJ_RS04595 at 856.882 kb on - strand, within MMJJ_RS04595 at 857.018 kb on + strand, within MMJJ_RS04595 at 857.024 kb on - strand, within MMJJ_RS04595 at 857.060 kb on + strand, within MMJJ_RS04595 at 857.122 kb on - strand, within MMJJ_RS04595 at 857.222 kb on + strand, within MMJJ_RS04595 at 857.274 kb on - strand at 857.275 kb on + strand at 857.450 kb on + strand, within MMJJ_RS04600 at 857.665 kb on - strand at 857.709 kb on - strand at 857.709 kb on - strand at 857.710 kb on + strand at 857.710 kb on + strand at 857.729 kb on - strand at 857.729 kb on - strand at 858.136 kb on + strand at 858.260 kb on - strand at 858.276 kb on + strand at 858.299 kb on - strand at 858.300 kb on + strand at 858.303 kb on + strand at 858.304 kb on - strand at 858.305 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 2g/L casaminos 10mM Ammonium chloride remove 855,794 - MMJJ_RS04585 0.47 +0.3 855,893 - MMJJ_RS04585 0.55 +0.6 855,943 + MMJJ_RS04585 0.59 +0.6 856,025 - MMJJ_RS04585 0.65 +1.0 856,025 - MMJJ_RS04585 0.65 +0.8 856,029 - MMJJ_RS04585 0.65 -0.3 856,038 - MMJJ_RS04585 0.66 +0.7 856,038 - MMJJ_RS04585 0.66 +0.5 856,039 + MMJJ_RS04585 0.66 +1.3 856,039 + MMJJ_RS04585 0.66 -0.0 856,062 - MMJJ_RS04585 0.68 -0.7 856,275 + MMJJ_RS04585 0.84 -1.4 856,294 + MMJJ_RS04585 0.86 -0.2 856,296 + MMJJ_RS04585 0.86 -0.4 856,365 - -0.1 856,366 + +1.0 856,406 - +0.1 856,417 + +0.4 856,417 + +0.2 856,441 + -0.1 856,478 + +0.4 856,491 + -0.3 856,725 + -0.5 856,769 - -2.4 856,769 - -2.5 856,882 - MMJJ_RS04595 0.27 -2.9 856,882 - MMJJ_RS04595 0.27 -1.2 857,018 + MMJJ_RS04595 0.49 -3.5 857,024 - MMJJ_RS04595 0.50 -3.4 857,060 + MMJJ_RS04595 0.56 -3.6 857,122 - MMJJ_RS04595 0.67 -1.2 857,222 + MMJJ_RS04595 0.83 -0.7 857,274 - -2.8 857,275 + -2.2 857,450 + MMJJ_RS04600 0.35 -2.6 857,665 - -4.8 857,709 - -1.7 857,709 - +0.9 857,710 + -2.4 857,710 + -3.3 857,729 - -3.4 857,729 - -2.8 858,136 + -2.4 858,260 - -2.1 858,276 + +0.5 858,299 - +0.5 858,300 + -2.4 858,303 + +0.2 858,304 - -1.7 858,305 + -0.7
Or see this region's nucleotide sequence