Experiment: 1-ethyl-3-methylimidazolium lysine 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_2778 and Shew_2779 are separated by 327 nucleotides Shew_2779 and Shew_2780 are separated by 86 nucleotides Shew_2780 and Shew_2781 are separated by 23 nucleotides
Shew_2778: Shew_2778 - diguanylate cyclase/phosphodiesterase with PAS/PAC and GAF sensor(s) (RefSeq), at 3,284,738 to 3,287,770
_2778
Shew_2779: Shew_2779 - amino acid ABC transporter (RefSeq), at 3,288,098 to 3,288,907
_2779
Shew_2780: Shew_2780 - thioesterase superfamily protein (RefSeq), at 3,288,994 to 3,289,434
_2780
Shew_2781: Shew_2781 - diguanylate cyclase/phosphodiesterase (RefSeq), at 3,289,458 to 3,290,954
_2781
Position (kb)
3288
3289 Strain fitness (log2 ratio)
-2
-1
0
1 at 3287.415 kb on + strand, within Shew_2778 at 3287.562 kb on - strand at 3287.916 kb on - strand at 3288.391 kb on + strand, within Shew_2779 at 3288.783 kb on + strand, within Shew_2779 at 3288.791 kb on - strand, within Shew_2779 at 3288.882 kb on + strand at 3288.890 kb on - strand at 3288.890 kb on - strand at 3289.075 kb on + strand, within Shew_2780 at 3289.603 kb on - strand at 3289.801 kb on + strand, within Shew_2781
Per-strain Table
Position Strand Gene LocusTag Fraction 1-ethyl-3-methylimidazolium lysine 10 mM remove 3,287,415 + Shew_2778 0.88 -1.0 3,287,562 - -0.1 3,287,916 - +0.1 3,288,391 + Shew_2779 0.36 -2.0 3,288,783 + Shew_2779 0.85 -2.2 3,288,791 - Shew_2779 0.86 -1.3 3,288,882 + +0.6 3,288,890 - -0.3 3,288,890 - +0.4 3,289,075 + Shew_2780 0.18 -0.9 3,289,603 - -0.7 3,289,801 + Shew_2781 0.23 -2.0
Or see this region's nucleotide sequence