Experiment: MoLS4 without vitamins with Casaminos 0.2%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt atoC and DVU3306 are separated by 229 nucleotides DVU3306 and tRNA-Arg are separated by 46 nucleotides tRNA-Arg and ubiX are separated by 243 nucleotides
DVU3305: atoC - sigma-54 dependent transcriptional regulator/response regulator (TIGR), at 3,482,737 to 3,484,092
atoC
DVU3306: DVU3306 - hypothetical protein (TIGR), at 3,484,322 to 3,484,486
DVU3306
DVU_tRNA-Arg-6: tRNA-Arg - tRNA with anticodon CCG for Arg (VIMSS-AUTO), at 3,484,533 to 3,484,609
tRNA-Arg
DVU3307: ubiX - 3-octaprenyl-4-hydroxybenzoate carboxy-lyase (TIGR), at 3,484,853 to 3,485,416
ubiX
Position (kb)
3484
3485 Strain fitness (log2 ratio)
-2
-1
0
1 at 3483.350 kb on - strand, within atoC at 3483.429 kb on - strand, within atoC at 3483.434 kb on - strand, within atoC at 3483.449 kb on - strand, within atoC at 3483.464 kb on + strand, within atoC at 3483.524 kb on - strand, within atoC at 3483.524 kb on - strand, within atoC at 3483.629 kb on - strand, within atoC at 3483.643 kb on + strand, within atoC at 3483.644 kb on + strand, within atoC at 3483.714 kb on + strand, within atoC at 3483.784 kb on - strand, within atoC at 3483.813 kb on + strand, within atoC at 3483.813 kb on + strand, within atoC at 3483.821 kb on - strand, within atoC at 3483.850 kb on + strand, within atoC at 3483.852 kb on + strand, within atoC at 3483.853 kb on - strand, within atoC at 3483.870 kb on + strand, within atoC at 3483.900 kb on + strand, within atoC at 3483.951 kb on - strand, within atoC at 3483.958 kb on - strand at 3483.970 kb on + strand at 3484.079 kb on - strand at 3484.175 kb on - strand at 3484.202 kb on + strand at 3484.288 kb on + strand at 3484.291 kb on - strand at 3484.291 kb on - strand at 3484.293 kb on + strand at 3484.293 kb on + strand at 3484.296 kb on - strand at 3484.312 kb on + strand at 3484.398 kb on - strand, within DVU3306 at 3484.398 kb on - strand, within DVU3306 at 3484.398 kb on - strand, within DVU3306 at 3484.399 kb on - strand, within DVU3306 at 3484.413 kb on - strand, within DVU3306 at 3484.656 kb on + strand at 3484.669 kb on - strand at 3484.669 kb on - strand at 3484.751 kb on - strand at 3484.850 kb on + strand at 3485.409 kb on + strand at 3485.417 kb on + strand at 3485.432 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 without vitamins with Casaminos 0.2% remove 3,483,350 - atoC DVU3305 0.45 +0.2 3,483,429 - atoC DVU3305 0.51 +0.3 3,483,434 - atoC DVU3305 0.51 -0.7 3,483,449 - atoC DVU3305 0.53 -0.1 3,483,464 + atoC DVU3305 0.54 -2.0 3,483,524 - atoC DVU3305 0.58 -0.5 3,483,524 - atoC DVU3305 0.58 -0.1 3,483,629 - atoC DVU3305 0.66 -0.0 3,483,643 + atoC DVU3305 0.67 +0.3 3,483,644 + atoC DVU3305 0.67 -0.4 3,483,714 + atoC DVU3305 0.72 +0.3 3,483,784 - atoC DVU3305 0.77 -0.2 3,483,813 + atoC DVU3305 0.79 -0.2 3,483,813 + atoC DVU3305 0.79 -0.9 3,483,821 - atoC DVU3305 0.80 +0.7 3,483,850 + atoC DVU3305 0.82 +0.1 3,483,852 + atoC DVU3305 0.82 +0.0 3,483,853 - atoC DVU3305 0.82 +0.2 3,483,870 + atoC DVU3305 0.84 -2.4 3,483,900 + atoC DVU3305 0.86 +0.4 3,483,951 - atoC DVU3305 0.90 +0.7 3,483,958 - -0.2 3,483,970 + +0.2 3,484,079 - -0.2 3,484,175 - -0.1 3,484,202 + -0.3 3,484,288 + -2.5 3,484,291 - +0.9 3,484,291 - -0.1 3,484,293 + +0.2 3,484,293 + -0.1 3,484,296 - -0.4 3,484,312 + -0.1 3,484,398 - DVU3306 0.46 +0.3 3,484,398 - DVU3306 0.46 +0.2 3,484,398 - DVU3306 0.46 -0.1 3,484,399 - DVU3306 0.47 -0.3 3,484,413 - DVU3306 0.55 +0.5 3,484,656 + -0.5 3,484,669 - -0.0 3,484,669 - -0.2 3,484,751 - -0.7 3,484,850 + -1.0 3,485,409 + +0.2 3,485,417 + +0.3 3,485,432 + +0.5
Or see this region's nucleotide sequence