Experiment: Formate N-free with 2mM Adenosine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS08130 and MMJJ_RS08135 are separated by 161 nucleotides MMJJ_RS08135 and MMJJ_RS08140 are separated by 210 nucleotides MMJJ_RS08140 and MMJJ_RS08145 are separated by 179 nucleotides
MMJJ_RS08130: MMJJ_RS08130 - translation initiation factor IF-2 subunit gamma, at 1,491,947 to 1,493,179
_RS08130
MMJJ_RS08135: MMJJ_RS08135 - 30S ribosomal protein S6e, at 1,493,341 to 1,493,715
_RS08135
MMJJ_RS08140: MMJJ_RS08140 - type I glutamate--ammonia ligase, at 1,493,926 to 1,495,266
_RS08140
MMJJ_RS08145: MMJJ_RS08145 - 3-phosphoshikimate 1-carboxyvinyltransferase, at 1,495,446 to 1,496,735
_RS08145
Position (kb)
1493
1494
1495
1496 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 1493.214 kb on - strand at 1493.283 kb on - strand at 1493.285 kb on - strand at 1493.285 kb on - strand at 1493.285 kb on - strand at 1493.285 kb on - strand at 1493.285 kb on - strand at 1493.286 kb on + strand at 1493.286 kb on + strand at 1493.286 kb on + strand at 1493.286 kb on + strand at 1493.286 kb on + strand at 1493.286 kb on + strand at 1493.286 kb on + strand at 1493.286 kb on + strand at 1493.289 kb on - strand at 1493.289 kb on - strand at 1493.289 kb on - strand at 1493.290 kb on + strand at 1493.290 kb on + strand at 1493.291 kb on - strand at 1493.291 kb on - strand at 1493.292 kb on + strand at 1493.292 kb on + strand at 1493.292 kb on + strand at 1493.319 kb on + strand at 1493.337 kb on - strand at 1493.407 kb on - strand, within MMJJ_RS08135 at 1493.412 kb on - strand, within MMJJ_RS08135 at 1493.412 kb on - strand, within MMJJ_RS08135 at 1493.413 kb on + strand, within MMJJ_RS08135 at 1493.413 kb on + strand, within MMJJ_RS08135 at 1493.413 kb on + strand, within MMJJ_RS08135 at 1493.458 kb on + strand, within MMJJ_RS08135 at 1493.503 kb on - strand, within MMJJ_RS08135 at 1493.504 kb on + strand, within MMJJ_RS08135 at 1493.593 kb on - strand, within MMJJ_RS08135 at 1493.718 kb on - strand at 1493.721 kb on - strand at 1493.742 kb on - strand at 1493.742 kb on - strand at 1493.742 kb on - strand at 1493.742 kb on - strand at 1493.743 kb on + strand at 1493.802 kb on - strand at 1493.821 kb on - strand at 1493.822 kb on + strand at 1493.828 kb on + strand at 1493.828 kb on + strand at 1493.832 kb on - strand at 1493.833 kb on + strand at 1493.836 kb on + strand at 1493.898 kb on - strand at 1493.899 kb on + strand at 1493.899 kb on + strand at 1493.914 kb on + strand at 1493.924 kb on + strand at 1493.928 kb on + strand at 1493.936 kb on + strand at 1493.936 kb on + strand at 1493.936 kb on + strand at 1495.239 kb on + strand at 1495.290 kb on - strand at 1495.322 kb on - strand at 1495.327 kb on + strand at 1495.340 kb on - strand at 1495.340 kb on - strand at 1495.340 kb on - strand at 1495.341 kb on + strand at 1495.341 kb on + strand at 1495.341 kb on + strand at 1495.341 kb on + strand at 1495.350 kb on - strand at 1495.350 kb on - strand at 1495.350 kb on - strand at 1495.350 kb on - strand at 1495.351 kb on + strand at 1495.351 kb on + strand at 1495.363 kb on + strand at 1495.363 kb on + strand at 1495.363 kb on + strand at 1495.382 kb on + strand at 1495.396 kb on - strand at 1495.403 kb on + strand at 1495.422 kb on + strand at 1495.427 kb on - strand at 1495.454 kb on + strand at 1495.556 kb on - strand at 1495.716 kb on + strand, within MMJJ_RS08145
Per-strain Table
Position Strand Gene LocusTag Fraction Formate N-free with 2mM Adenosine remove 1,493,214 - -0.3 1,493,283 - -1.0 1,493,285 - -2.6 1,493,285 - -0.3 1,493,285 - -2.4 1,493,285 - -3.1 1,493,285 - +2.4 1,493,286 + -2.8 1,493,286 + -1.7 1,493,286 + +1.0 1,493,286 + -1.8 1,493,286 + -0.8 1,493,286 + -1.1 1,493,286 + -2.5 1,493,286 + -2.0 1,493,289 - -0.1 1,493,289 - -1.8 1,493,289 - -1.0 1,493,290 + +1.3 1,493,290 + -0.8 1,493,291 - -3.9 1,493,291 - -0.8 1,493,292 + +0.0 1,493,292 + -1.8 1,493,292 + -2.0 1,493,319 + -2.2 1,493,337 - +3.6 1,493,407 - MMJJ_RS08135 0.18 +1.0 1,493,412 - MMJJ_RS08135 0.19 -2.4 1,493,412 - MMJJ_RS08135 0.19 -4.1 1,493,413 + MMJJ_RS08135 0.19 -1.4 1,493,413 + MMJJ_RS08135 0.19 -0.6 1,493,413 + MMJJ_RS08135 0.19 -0.7 1,493,458 + MMJJ_RS08135 0.31 +1.2 1,493,503 - MMJJ_RS08135 0.43 -0.4 1,493,504 + MMJJ_RS08135 0.43 +1.8 1,493,593 - MMJJ_RS08135 0.67 -0.3 1,493,718 - -1.1 1,493,721 - -0.1 1,493,742 - -1.1 1,493,742 - -0.3 1,493,742 - +3.2 1,493,742 - -0.6 1,493,743 + -1.6 1,493,802 - -2.5 1,493,821 - -1.1 1,493,822 + +0.4 1,493,828 + -0.7 1,493,828 + +1.5 1,493,832 - +1.8 1,493,833 + +1.1 1,493,836 + -1.7 1,493,898 - +0.0 1,493,899 + -0.1 1,493,899 + -1.8 1,493,914 + +1.3 1,493,924 + +1.1 1,493,928 + +1.5 1,493,936 + -0.8 1,493,936 + +0.1 1,493,936 + -2.3 1,495,239 + +2.0 1,495,290 - -2.0 1,495,322 - +0.0 1,495,327 + -0.6 1,495,340 - -0.4 1,495,340 - -2.5 1,495,340 - +1.1 1,495,341 + +0.4 1,495,341 + +0.3 1,495,341 + +1.4 1,495,341 + +0.3 1,495,350 - +0.3 1,495,350 - +1.4 1,495,350 - +0.3 1,495,350 - +0.0 1,495,351 + -0.4 1,495,351 + +2.1 1,495,363 + -0.6 1,495,363 + -1.3 1,495,363 + +0.0 1,495,382 + -2.0 1,495,396 - -2.6 1,495,403 + -0.1 1,495,422 + +0.6 1,495,427 - +1.4 1,495,454 + -0.3 1,495,556 - -1.9 1,495,716 + MMJJ_RS08145 0.21 -0.3
Or see this region's nucleotide sequence