Experiment: Formate N-free with 2mM Adenosine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS06310 and MMJJ_RS06315 are separated by 16 nucleotides MMJJ_RS06315 and MMJJ_RS06320 are separated by 21 nucleotides MMJJ_RS06320 and MMJJ_RS06325 are separated by 10 nucleotides MMJJ_RS06325 and MMJJ_RS06330 are separated by 36 nucleotides
MMJJ_RS06310: MMJJ_RS06310 - tetrahydromethanopterin S-methyltransferase subunit A, at 1,169,338 to 1,170,099
_RS06310
MMJJ_RS06315: MMJJ_RS06315 - tetrahydromethanopterin S-methyltransferase subunit A, at 1,170,116 to 1,170,835
_RS06315
MMJJ_RS06320: MMJJ_RS06320 - tetrahydromethanopterin S-methyltransferase subunit B, at 1,170,857 to 1,171,183
_RS06320
MMJJ_RS06325: MMJJ_RS06325 - tetrahydromethanopterin S-methyltransferase subunit C, at 1,171,194 to 1,171,982
_RS06325
MMJJ_RS06330: MMJJ_RS06330 - tetrahydromethanopterin S-methyltransferase subunit D, at 1,172,019 to 1,172,726
_RS06330
Position (kb) 1170
1171
1172 Strain fitness (log2 ratio) -1
0
1 at 1170.858 kb on - strand at 1171.166 kb on - strand at 1171.779 kb on + strand, within MMJJ_RS06325 at 1171.863 kb on + strand, within MMJJ_RS06325 at 1171.987 kb on + strand at 1171.987 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Formate N-free with 2mM Adenosine remove 1,170,858 - -0.3 1,171,166 - -0.8 1,171,779 + MMJJ_RS06325 0.74 -0.6 1,171,863 + MMJJ_RS06325 0.85 +0.4 1,171,987 + +0.4 1,171,987 + +0.4
Or see this region's nucleotide sequence