Experiment: LB_plus_SM_buffer
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1240 and GFF1241 are separated by 269 nucleotides GFF1241 and GFF1242 are separated by 207 nucleotides
GFF1240 - Virulence genes transcriptional activator, at 71,644 to 72,537
GFF1240
GFF1241 - hypothetical protein, at 72,807 to 72,938
GFF1241
GFF1242 - Mobile element protein, at 73,146 to 74,183
GFF1242
Position (kb)
72
73 Strain fitness (log2 ratio)
-1
0
1 at 71.819 kb on - strand, within GFF1240 at 71.872 kb on - strand, within GFF1240 at 71.895 kb on + strand, within GFF1240 at 71.899 kb on - strand, within GFF1240 at 71.902 kb on + strand, within GFF1240 at 72.451 kb on - strand at 72.458 kb on - strand at 72.687 kb on - strand at 72.732 kb on + strand at 72.732 kb on + strand at 72.732 kb on + strand at 72.733 kb on - strand at 72.756 kb on - strand at 72.883 kb on + strand, within GFF1241 at 72.902 kb on + strand, within GFF1241 at 72.937 kb on - strand at 73.049 kb on - strand at 73.049 kb on - strand at 73.049 kb on - strand at 73.062 kb on - strand at 73.062 kb on - strand at 73.156 kb on + strand at 73.156 kb on + strand at 73.156 kb on + strand at 73.328 kb on - strand, within GFF1242 at 73.342 kb on + strand, within GFF1242 at 73.361 kb on - strand, within GFF1242 at 73.407 kb on - strand, within GFF1242 at 73.432 kb on + strand, within GFF1242 at 73.548 kb on + strand, within GFF1242 at 73.565 kb on + strand, within GFF1242 at 73.614 kb on + strand, within GFF1242 at 73.894 kb on - strand, within GFF1242 at 73.929 kb on - strand, within GFF1242 at 73.932 kb on + strand, within GFF1242 at 73.933 kb on - strand, within GFF1242 at 73.933 kb on - strand, within GFF1242 at 73.933 kb on - strand, within GFF1242
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer remove 71,819 - GFF1240 0.20 +0.4 71,872 - GFF1240 0.26 -0.3 71,895 + GFF1240 0.28 -0.6 71,899 - GFF1240 0.29 +0.0 71,902 + GFF1240 0.29 +0.1 72,451 - -0.0 72,458 - -0.5 72,687 - +0.4 72,732 + +0.0 72,732 + +0.0 72,732 + -0.1 72,733 - +0.2 72,756 - +0.9 72,883 + GFF1241 0.58 +0.1 72,902 + GFF1241 0.72 +0.5 72,937 - +0.3 73,049 - -0.3 73,049 - -0.1 73,049 - -0.6 73,062 - +0.5 73,062 - -0.5 73,156 + -0.6 73,156 + +0.3 73,156 + -0.4 73,328 - GFF1242 0.18 -0.4 73,342 + GFF1242 0.19 -1.1 73,361 - GFF1242 0.21 +0.2 73,407 - GFF1242 0.25 +0.0 73,432 + GFF1242 0.28 +0.0 73,548 + GFF1242 0.39 -0.8 73,565 + GFF1242 0.40 -0.1 73,614 + GFF1242 0.45 -0.1 73,894 - GFF1242 0.72 -0.5 73,929 - GFF1242 0.75 +0.7 73,932 + GFF1242 0.76 +0.1 73,933 - GFF1242 0.76 +0.3 73,933 - GFF1242 0.76 +0.3 73,933 - GFF1242 0.76 +0.1
Or see this region's nucleotide sequence