Experiment: LB_plus_SM_buffer with Ffm_phage 0.000001875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF286 and GFF287 are separated by 161 nucleotides GFF287 and GFF288 overlap by 19 nucleotides GFF288 and GFF289 are separated by 19 nucleotides GFF289 and GFF290 overlap by 11 nucleotides GFF290 and GFF291 overlap by 4 nucleotides
GFF286 - Mediator of hyperadherence YidE, at 3,890 to 5,551
GFF286
GFF287 - hypothetical protein, at 5,713 to 5,859
GFF287
GFF288 - hypothetical protein, at 5,841 to 5,975
GFF288
GFF289 - Putative inner membrane protein, at 5,995 to 6,342
GFF289
GFF290 - Putative inner membrane protein, at 6,332 to 6,694
GFF290
GFF291 - Putative arylsulfatase regulatory protein, at 6,691 to 7,221
GFF291
Position (kb) 5
6 Strain fitness (log2 ratio) -1
0
1 at 4.756 kb on - strand, within GFF286 at 4.823 kb on - strand, within GFF286 at 5.028 kb on + strand, within GFF286 at 5.156 kb on + strand, within GFF286 at 5.200 kb on - strand, within GFF286 at 5.295 kb on - strand, within GFF286 at 5.299 kb on + strand, within GFF286 at 5.299 kb on + strand, within GFF286 at 5.503 kb on + strand at 5.503 kb on + strand at 5.504 kb on - strand at 5.592 kb on + strand at 5.714 kb on + strand at 5.818 kb on - strand, within GFF287 at 5.878 kb on + strand, within GFF288 at 5.885 kb on - strand, within GFF288 at 5.895 kb on - strand, within GFF288 at 5.970 kb on - strand at 5.973 kb on + strand at 5.973 kb on + strand at 5.973 kb on + strand at 5.974 kb on - strand at 5.974 kb on - strand at 6.199 kb on + strand, within GFF289 at 6.200 kb on - strand, within GFF289 at 6.200 kb on - strand, within GFF289 at 6.209 kb on + strand, within GFF289 at 6.261 kb on + strand, within GFF289 at 6.267 kb on + strand, within GFF289 at 6.311 kb on + strand at 6.347 kb on - strand at 6.388 kb on + strand, within GFF290 at 6.389 kb on - strand, within GFF290 at 6.422 kb on + strand, within GFF290 at 6.532 kb on + strand, within GFF290 at 6.688 kb on + strand at 6.789 kb on - strand, within GFF291 at 6.789 kb on - strand, within GFF291 at 6.820 kb on - strand, within GFF291
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Ffm_phage 0.000001875 MOI remove 4,756 - GFF286 0.52 +1.1 4,823 - GFF286 0.56 -0.2 5,028 + GFF286 0.68 +0.2 5,156 + GFF286 0.76 -1.1 5,200 - GFF286 0.79 +0.1 5,295 - GFF286 0.85 -0.1 5,299 + GFF286 0.85 +0.1 5,299 + GFF286 0.85 +0.1 5,503 + +0.3 5,503 + +0.0 5,504 - -0.0 5,592 + -0.2 5,714 + -0.3 5,818 - GFF287 0.71 -1.2 5,878 + GFF288 0.27 +0.2 5,885 - GFF288 0.33 +0.2 5,895 - GFF288 0.40 -0.6 5,970 - +0.3 5,973 + -0.1 5,973 + -0.1 5,973 + -0.3 5,974 - +0.4 5,974 - -0.0 6,199 + GFF289 0.59 +0.5 6,200 - GFF289 0.59 +0.7 6,200 - GFF289 0.59 -0.9 6,209 + GFF289 0.61 +0.2 6,261 + GFF289 0.76 -0.8 6,267 + GFF289 0.78 -0.3 6,311 + +0.9 6,347 - +1.2 6,388 + GFF290 0.15 +0.1 6,389 - GFF290 0.16 +0.0 6,422 + GFF290 0.25 -0.2 6,532 + GFF290 0.55 +0.2 6,688 + +0.7 6,789 - GFF291 0.18 -0.3 6,789 - GFF291 0.18 -0.3 6,820 - GFF291 0.24 -0.0
Or see this region's nucleotide sequence