Experiment: LB_plus_SM_buffer with Ffm_phage 0.000001875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2218 and GFF2219 are separated by 188 nucleotides GFF2219 and GFF2220 are separated by 312 nucleotides
GFF2218 - Protein of unknown function DUF414, at 9,391 to 9,906
GFF2218
GFF2219 - Coproporphyrinogen III oxidase, oxygen-independent (EC 1.3.99.22), at 10,095 to 11,468
GFF2219
GFF2220 - Nitrogen regulation protein NR(I), at 11,781 to 13,190
GFF2220
Position (kb)
10
11
12 Strain fitness (log2 ratio)
-2
-1
0
1 at 9.181 kb on + strand at 9.181 kb on + strand at 9.181 kb on + strand at 9.184 kb on + strand at 9.190 kb on + strand at 9.586 kb on - strand, within GFF2218 at 9.642 kb on + strand, within GFF2218 at 9.725 kb on + strand, within GFF2218 at 9.776 kb on + strand, within GFF2218 at 9.877 kb on - strand at 9.878 kb on - strand at 9.939 kb on + strand at 9.940 kb on - strand at 9.956 kb on + strand at 9.956 kb on + strand at 9.961 kb on + strand at 10.140 kb on + strand at 10.141 kb on - strand at 10.141 kb on - strand at 10.142 kb on + strand at 10.143 kb on - strand at 10.143 kb on - strand at 10.147 kb on - strand at 10.161 kb on + strand at 10.163 kb on + strand at 10.163 kb on + strand at 10.163 kb on + strand at 10.261 kb on - strand, within GFF2219 at 10.439 kb on + strand, within GFF2219 at 10.440 kb on - strand, within GFF2219 at 10.440 kb on - strand, within GFF2219 at 10.452 kb on - strand, within GFF2219 at 10.471 kb on + strand, within GFF2219 at 10.617 kb on - strand, within GFF2219 at 10.704 kb on - strand, within GFF2219 at 10.815 kb on - strand, within GFF2219 at 10.815 kb on - strand, within GFF2219 at 10.818 kb on + strand, within GFF2219 at 10.818 kb on + strand, within GFF2219 at 10.819 kb on - strand, within GFF2219 at 10.819 kb on - strand, within GFF2219 at 10.899 kb on - strand, within GFF2219 at 11.100 kb on + strand, within GFF2219 at 11.101 kb on - strand, within GFF2219 at 11.289 kb on + strand, within GFF2219 at 11.312 kb on - strand, within GFF2219 at 11.348 kb on + strand at 11.657 kb on - strand at 11.669 kb on - strand at 11.720 kb on + strand at 11.754 kb on - strand at 11.878 kb on - strand at 11.977 kb on - strand, within GFF2220 at 12.063 kb on + strand, within GFF2220 at 12.064 kb on - strand, within GFF2220 at 12.406 kb on + strand, within GFF2220 at 12.406 kb on + strand, within GFF2220 at 12.407 kb on - strand, within GFF2220
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Ffm_phage 0.000001875 MOI remove 9,181 + -0.5 9,181 + -0.2 9,181 + -0.3 9,184 + +0.1 9,190 + -0.0 9,586 - GFF2218 0.38 -0.9 9,642 + GFF2218 0.49 -0.2 9,725 + GFF2218 0.65 -0.0 9,776 + GFF2218 0.75 +0.2 9,877 - -0.6 9,878 - +0.0 9,939 + +0.0 9,940 - +1.6 9,956 + +0.0 9,956 + -0.4 9,961 + -0.8 10,140 + -0.9 10,141 - -0.6 10,141 - +0.0 10,142 + -0.7 10,143 - -1.0 10,143 - -1.2 10,147 - -0.5 10,161 + +0.1 10,163 + -0.5 10,163 + -1.1 10,163 + -0.4 10,261 - GFF2219 0.12 +0.4 10,439 + GFF2219 0.25 -0.9 10,440 - GFF2219 0.25 +0.8 10,440 - GFF2219 0.25 +0.1 10,452 - GFF2219 0.26 +0.2 10,471 + GFF2219 0.27 -1.6 10,617 - GFF2219 0.38 -1.8 10,704 - GFF2219 0.44 -0.4 10,815 - GFF2219 0.52 -2.5 10,815 - GFF2219 0.52 -0.6 10,818 + GFF2219 0.53 -1.1 10,818 + GFF2219 0.53 -0.5 10,819 - GFF2219 0.53 -0.3 10,819 - GFF2219 0.53 -0.1 10,899 - GFF2219 0.59 -0.2 11,100 + GFF2219 0.73 -0.8 11,101 - GFF2219 0.73 -1.0 11,289 + GFF2219 0.87 -1.3 11,312 - GFF2219 0.89 -0.0 11,348 + +0.6 11,657 - -0.3 11,669 - +0.2 11,720 + +0.1 11,754 - +0.9 11,878 - -1.2 11,977 - GFF2220 0.14 -0.5 12,063 + GFF2220 0.20 -1.5 12,064 - GFF2220 0.20 -0.3 12,406 + GFF2220 0.44 -1.1 12,406 + GFF2220 0.44 -1.5 12,407 - GFF2220 0.44 -2.7
Or see this region's nucleotide sequence