Experiment: m.b. Cisplatin 0.05 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_1100 and Dshi_1101 are separated by 64 nucleotides Dshi_1101 and Dshi_1102 are separated by 76 nucleotides Dshi_1102 and Dshi_1103 are separated by 312 nucleotides Dshi_1103 and Dshi_1104 overlap by 4 nucleotides Dshi_1104 and Dshi_1105 are separated by 3 nucleotides
Dshi_1100: Dshi_1100 - ribosomal L11 methyltransferase (RefSeq), at 1,128,118 to 1,128,987
_1100
Dshi_1101: Dshi_1101 - hypothetical protein (RefSeq), at 1,129,052 to 1,129,375
_1101
Dshi_1102: Dshi_1102 - protein of unknown function DUF1127 (RefSeq), at 1,129,452 to 1,129,649
_1102
Dshi_1103: Dshi_1103 - crossover junction endodeoxyribonuclease RuvC (RefSeq), at 1,129,962 to 1,130,492
_1103
Dshi_1104: Dshi_1104 - Holliday junction DNA helicase RuvA (RefSeq), at 1,130,489 to 1,131,163
_1104
Dshi_1105: Dshi_1105 - Holliday junction DNA helicase RuvB (RefSeq), at 1,131,167 to 1,132,240
_1105
Position (kb)
1129
1130
1131 Strain fitness (log2 ratio)
-2
-1
0
1 at 1129.107 kb on - strand, within Dshi_1101 at 1129.146 kb on - strand, within Dshi_1101 at 1129.716 kb on + strand at 1129.724 kb on - strand at 1129.724 kb on - strand at 1129.783 kb on + strand at 1129.789 kb on - strand at 1129.789 kb on - strand at 1129.791 kb on - strand at 1129.827 kb on - strand at 1129.868 kb on - strand at 1129.898 kb on + strand at 1130.096 kb on - strand, within Dshi_1103 at 1130.191 kb on + strand, within Dshi_1103 at 1130.413 kb on + strand, within Dshi_1103 at 1130.773 kb on + strand, within Dshi_1104 at 1130.890 kb on + strand, within Dshi_1104
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Cisplatin 0.05 mg/ml remove 1,129,107 - Dshi_1101 0.17 -0.7 1,129,146 - Dshi_1101 0.29 +0.0 1,129,716 + +0.7 1,129,724 - -1.5 1,129,724 - -0.6 1,129,783 + -1.9 1,129,789 - -2.2 1,129,789 - +1.3 1,129,791 - -0.5 1,129,827 - -1.4 1,129,868 - -0.5 1,129,898 + -0.3 1,130,096 - Dshi_1103 0.25 -1.5 1,130,191 + Dshi_1103 0.43 -0.7 1,130,413 + Dshi_1103 0.85 -1.3 1,130,773 + Dshi_1104 0.42 -0.7 1,130,890 + Dshi_1104 0.59 -1.3
Or see this region's nucleotide sequence