Experiment: H2 N-free with 2mM Adenosine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS02930 and MMJJ_RS02935 are separated by 31 nucleotides MMJJ_RS02935 and MMJJ_RS02940 are separated by 47 nucleotides MMJJ_RS02940 and MMJJ_RS02945 are separated by 155 nucleotides MMJJ_RS02945 and MMJJ_RS02950 are separated by 93 nucleotides MMJJ_RS02950 and MMJJ_RS02955 are separated by 3 nucleotides
MMJJ_RS02930: MMJJ_RS02930 - DUF359 domain-containing protein, at 547,996 to 548,472
_RS02930
MMJJ_RS02935: MMJJ_RS02935 - DNA-directed RNA polymerase subunit E'', at 548,504 to 548,680
_RS02935
MMJJ_RS02940: MMJJ_RS02940 - DNA-directed RNA polymerase, at 548,728 to 549,291
_RS02940
MMJJ_RS02945: MMJJ_RS02945 - dihydroorotate dehydrogenase, at 549,447 to 550,361
_RS02945
MMJJ_RS02950: MMJJ_RS02950 - epoxyqueuosine reductase, at 550,455 to 551,246
_RS02950
MMJJ_RS02955: MMJJ_RS02955 - glutamate-cysteine ligase family protein, at 551,250 to 552,353
_RS02955
Position (kb)
549
550
551 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 548.468 kb on - strand at 548.483 kb on - strand at 548.484 kb on + strand at 548.484 kb on + strand at 548.484 kb on + strand at 548.484 kb on + strand at 548.485 kb on - strand at 548.486 kb on + strand at 548.486 kb on + strand at 548.500 kb on - strand at 548.505 kb on - strand at 548.506 kb on + strand at 548.506 kb on + strand at 548.506 kb on + strand at 548.534 kb on - strand, within MMJJ_RS02935 at 548.534 kb on - strand, within MMJJ_RS02935 at 548.535 kb on + strand, within MMJJ_RS02935 at 548.667 kb on + strand at 548.708 kb on - strand at 548.709 kb on + strand at 548.743 kb on - strand at 549.321 kb on - strand at 549.335 kb on - strand at 549.353 kb on - strand at 549.356 kb on - strand at 549.370 kb on - strand at 549.370 kb on - strand at 549.370 kb on - strand at 549.372 kb on - strand at 549.372 kb on - strand at 549.373 kb on + strand at 549.374 kb on - strand at 549.374 kb on - strand at 549.374 kb on - strand at 549.374 kb on - strand at 549.374 kb on - strand at 549.374 kb on - strand at 549.375 kb on + strand at 549.375 kb on + strand at 549.375 kb on + strand at 549.376 kb on - strand at 549.376 kb on - strand at 549.376 kb on - strand at 549.376 kb on - strand at 549.376 kb on - strand at 549.377 kb on + strand at 549.377 kb on + strand at 549.377 kb on + strand at 549.392 kb on + strand at 549.395 kb on - strand at 549.396 kb on + strand at 549.416 kb on + strand at 549.418 kb on + strand at 549.667 kb on + strand, within MMJJ_RS02945 at 549.673 kb on - strand, within MMJJ_RS02945 at 550.076 kb on - strand, within MMJJ_RS02945 at 550.239 kb on + strand at 550.398 kb on - strand at 550.405 kb on - strand at 550.407 kb on - strand at 550.407 kb on - strand at 550.427 kb on - strand at 550.428 kb on + strand at 550.431 kb on - strand at 550.431 kb on - strand at 550.431 kb on - strand at 550.431 kb on - strand at 550.431 kb on - strand at 550.431 kb on - strand at 550.431 kb on - strand at 550.431 kb on - strand at 550.431 kb on - strand at 550.432 kb on + strand at 550.432 kb on + strand at 550.432 kb on + strand at 550.432 kb on + strand at 550.432 kb on + strand at 550.432 kb on + strand at 550.432 kb on + strand at 550.432 kb on + strand at 550.432 kb on + strand at 550.432 kb on + strand at 550.440 kb on + strand at 550.455 kb on + strand at 550.465 kb on - strand at 550.526 kb on - strand at 550.546 kb on - strand, within MMJJ_RS02950 at 550.579 kb on - strand, within MMJJ_RS02950 at 550.585 kb on - strand, within MMJJ_RS02950 at 550.651 kb on - strand, within MMJJ_RS02950 at 550.651 kb on - strand, within MMJJ_RS02950 at 550.682 kb on - strand, within MMJJ_RS02950 at 550.683 kb on + strand, within MMJJ_RS02950 at 550.696 kb on - strand, within MMJJ_RS02950 at 550.696 kb on - strand, within MMJJ_RS02950 at 550.751 kb on - strand, within MMJJ_RS02950 at 550.751 kb on - strand, within MMJJ_RS02950 at 550.752 kb on + strand, within MMJJ_RS02950 at 550.752 kb on + strand, within MMJJ_RS02950 at 550.752 kb on + strand, within MMJJ_RS02950 at 550.805 kb on - strand, within MMJJ_RS02950 at 550.805 kb on - strand, within MMJJ_RS02950 at 550.805 kb on - strand, within MMJJ_RS02950 at 550.806 kb on + strand, within MMJJ_RS02950 at 550.806 kb on + strand, within MMJJ_RS02950 at 550.860 kb on - strand, within MMJJ_RS02950 at 550.860 kb on - strand, within MMJJ_RS02950 at 550.897 kb on - strand, within MMJJ_RS02950 at 550.897 kb on - strand, within MMJJ_RS02950 at 550.898 kb on + strand, within MMJJ_RS02950 at 550.898 kb on + strand, within MMJJ_RS02950 at 550.901 kb on - strand, within MMJJ_RS02950 at 550.902 kb on + strand, within MMJJ_RS02950 at 550.919 kb on - strand, within MMJJ_RS02950 at 550.919 kb on - strand, within MMJJ_RS02950 at 550.919 kb on - strand, within MMJJ_RS02950 at 550.920 kb on + strand, within MMJJ_RS02950 at 550.920 kb on + strand, within MMJJ_RS02950 at 550.920 kb on + strand, within MMJJ_RS02950 at 550.924 kb on - strand, within MMJJ_RS02950 at 550.924 kb on - strand, within MMJJ_RS02950 at 550.929 kb on + strand, within MMJJ_RS02950 at 550.944 kb on + strand, within MMJJ_RS02950 at 550.964 kb on + strand, within MMJJ_RS02950 at 550.967 kb on - strand, within MMJJ_RS02950 at 550.967 kb on - strand, within MMJJ_RS02950 at 550.968 kb on + strand, within MMJJ_RS02950 at 550.968 kb on + strand, within MMJJ_RS02950 at 550.988 kb on - strand, within MMJJ_RS02950 at 551.021 kb on - strand, within MMJJ_RS02950 at 551.021 kb on - strand, within MMJJ_RS02950 at 551.021 kb on - strand, within MMJJ_RS02950 at 551.022 kb on + strand, within MMJJ_RS02950 at 551.022 kb on + strand, within MMJJ_RS02950 at 551.025 kb on - strand, within MMJJ_RS02950 at 551.106 kb on - strand, within MMJJ_RS02950 at 551.113 kb on - strand, within MMJJ_RS02950 at 551.223 kb on - strand at 551.225 kb on - strand at 551.225 kb on - strand at 551.298 kb on - strand at 551.298 kb on - strand at 551.309 kb on - strand at 551.309 kb on - strand at 551.333 kb on - strand at 551.339 kb on - strand at 551.339 kb on - strand at 551.339 kb on - strand at 551.340 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 2mM Adenosine remove 548,468 - -0.7 548,483 - -1.7 548,484 + -0.0 548,484 + +0.8 548,484 + -0.2 548,484 + -0.9 548,485 - -0.7 548,486 + -0.7 548,486 + +0.6 548,500 - -1.2 548,505 - +2.4 548,506 + -1.0 548,506 + -0.5 548,506 + +0.0 548,534 - MMJJ_RS02935 0.17 -0.8 548,534 - MMJJ_RS02935 0.17 +0.0 548,535 + MMJJ_RS02935 0.18 -0.2 548,667 + +1.2 548,708 - -2.0 548,709 + +1.6 548,743 - -2.0 549,321 - -1.0 549,335 - -1.4 549,353 - -1.6 549,356 - -0.1 549,370 - +1.0 549,370 - -0.3 549,370 - -0.4 549,372 - -2.4 549,372 - -0.4 549,373 + +1.2 549,374 - -0.4 549,374 - -2.0 549,374 - -1.7 549,374 - -1.6 549,374 - -0.7 549,374 - -1.0 549,375 + -1.4 549,375 + +0.9 549,375 + +0.4 549,376 - -1.1 549,376 - -1.0 549,376 - +1.3 549,376 - -1.6 549,376 - -0.9 549,377 + -1.5 549,377 + -2.2 549,377 + -3.1 549,392 + -1.8 549,395 - -0.2 549,396 + -0.5 549,416 + +0.1 549,418 + +0.4 549,667 + MMJJ_RS02945 0.24 +0.9 549,673 - MMJJ_RS02945 0.25 -0.6 550,076 - MMJJ_RS02945 0.69 +0.4 550,239 + +1.6 550,398 - -1.8 550,405 - -0.1 550,407 - +0.9 550,407 - +0.0 550,427 - -0.5 550,428 + -0.6 550,431 - +0.1 550,431 - -1.2 550,431 - -0.5 550,431 - +0.1 550,431 - +0.9 550,431 - +1.0 550,431 - -2.8 550,431 - -2.1 550,431 - -1.2 550,432 + -0.7 550,432 + +1.0 550,432 + -0.1 550,432 + +0.2 550,432 + -0.7 550,432 + -0.3 550,432 + -0.8 550,432 + +1.2 550,432 + +0.9 550,432 + -1.5 550,440 + -0.6 550,455 + -1.5 550,465 - +1.0 550,526 - -0.2 550,546 - MMJJ_RS02950 0.11 +0.1 550,579 - MMJJ_RS02950 0.16 +0.9 550,585 - MMJJ_RS02950 0.16 -0.4 550,651 - MMJJ_RS02950 0.25 -1.7 550,651 - MMJJ_RS02950 0.25 -1.2 550,682 - MMJJ_RS02950 0.29 +2.7 550,683 + MMJJ_RS02950 0.29 -0.4 550,696 - MMJJ_RS02950 0.30 +0.3 550,696 - MMJJ_RS02950 0.30 +1.2 550,751 - MMJJ_RS02950 0.37 -0.6 550,751 - MMJJ_RS02950 0.37 +0.1 550,752 + MMJJ_RS02950 0.38 -1.2 550,752 + MMJJ_RS02950 0.38 -1.1 550,752 + MMJJ_RS02950 0.38 -2.3 550,805 - MMJJ_RS02950 0.44 +0.5 550,805 - MMJJ_RS02950 0.44 +0.6 550,805 - MMJJ_RS02950 0.44 +0.8 550,806 + MMJJ_RS02950 0.44 +0.4 550,806 + MMJJ_RS02950 0.44 +0.2 550,860 - MMJJ_RS02950 0.51 -0.1 550,860 - MMJJ_RS02950 0.51 +0.9 550,897 - MMJJ_RS02950 0.56 +0.5 550,897 - MMJJ_RS02950 0.56 -0.7 550,898 + MMJJ_RS02950 0.56 -1.3 550,898 + MMJJ_RS02950 0.56 -0.1 550,901 - MMJJ_RS02950 0.56 -2.2 550,902 + MMJJ_RS02950 0.56 +2.9 550,919 - MMJJ_RS02950 0.59 -3.1 550,919 - MMJJ_RS02950 0.59 +1.3 550,919 - MMJJ_RS02950 0.59 -1.3 550,920 + MMJJ_RS02950 0.59 -1.0 550,920 + MMJJ_RS02950 0.59 -1.2 550,920 + MMJJ_RS02950 0.59 -0.3 550,924 - MMJJ_RS02950 0.59 +0.1 550,924 - MMJJ_RS02950 0.59 -1.0 550,929 + MMJJ_RS02950 0.60 +0.4 550,944 + MMJJ_RS02950 0.62 -0.6 550,964 + MMJJ_RS02950 0.64 -0.8 550,967 - MMJJ_RS02950 0.65 +0.1 550,967 - MMJJ_RS02950 0.65 -0.1 550,968 + MMJJ_RS02950 0.65 -0.5 550,968 + MMJJ_RS02950 0.65 +0.7 550,988 - MMJJ_RS02950 0.67 +1.6 551,021 - MMJJ_RS02950 0.71 +0.8 551,021 - MMJJ_RS02950 0.71 +3.4 551,021 - MMJJ_RS02950 0.71 +0.9 551,022 + MMJJ_RS02950 0.72 +0.1 551,022 + MMJJ_RS02950 0.72 -0.5 551,025 - MMJJ_RS02950 0.72 -0.2 551,106 - MMJJ_RS02950 0.82 -0.6 551,113 - MMJJ_RS02950 0.83 +0.1 551,223 - +0.1 551,225 - -0.1 551,225 - +0.9 551,298 - +0.1 551,298 - -0.1 551,309 - -0.2 551,309 - -0.5 551,333 - +3.7 551,339 - +0.9 551,339 - -1.7 551,339 - -0.3 551,340 + +0.9
Or see this region's nucleotide sequence