Experiment: Polymyxin B sulfate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_1312 and Psest_1313 are separated by 199 nucleotides Psest_1313 and Psest_1314 are separated by 59 nucleotides Psest_1314 and Psest_1315 overlap by 14 nucleotides Psest_1315 and Psest_1316 overlap by 4 nucleotides
Psest_1312: Psest_1312 - ABC-type branched-chain amino acid transport systems, periplasmic component, at 1,413,061 to 1,414,179
_1312
Psest_1313: Psest_1313 - Uncharacterized conserved small protein, at 1,414,379 to 1,414,696
_1313
Psest_1314: Psest_1314 - Putative Zn-dependent protease, at 1,414,756 to 1,416,339
_1314
Psest_1315: Psest_1315 - hypothetical protein, at 1,416,326 to 1,416,628
_1315
Psest_1316: Psest_1316 - Predicted transmembrane sensor domain, at 1,416,625 to 1,417,983
_1316
Position (kb)
1414
1415
1416
1417 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 1413.836 kb on + strand, within Psest_1312 at 1413.836 kb on + strand, within Psest_1312 at 1413.837 kb on - strand, within Psest_1312 at 1413.837 kb on - strand, within Psest_1312 at 1414.113 kb on + strand at 1414.113 kb on + strand at 1414.115 kb on + strand at 1414.115 kb on + strand at 1414.115 kb on + strand at 1414.115 kb on + strand at 1414.116 kb on - strand at 1414.179 kb on + strand at 1414.179 kb on + strand at 1414.179 kb on + strand at 1414.179 kb on + strand at 1414.179 kb on + strand at 1414.179 kb on + strand at 1414.180 kb on - strand at 1414.180 kb on - strand at 1414.180 kb on - strand at 1414.180 kb on - strand at 1414.180 kb on - strand at 1414.180 kb on - strand at 1414.180 kb on - strand at 1414.180 kb on - strand at 1414.280 kb on + strand at 1414.281 kb on - strand at 1414.281 kb on - strand at 1414.364 kb on + strand at 1414.403 kb on + strand at 1414.404 kb on - strand at 1414.479 kb on + strand, within Psest_1313 at 1414.479 kb on + strand, within Psest_1313 at 1414.479 kb on + strand, within Psest_1313 at 1414.480 kb on - strand, within Psest_1313 at 1414.480 kb on - strand, within Psest_1313 at 1414.848 kb on + strand at 1414.848 kb on + strand at 1414.956 kb on - strand, within Psest_1314 at 1414.973 kb on + strand, within Psest_1314 at 1414.973 kb on + strand, within Psest_1314 at 1414.974 kb on - strand, within Psest_1314 at 1414.974 kb on - strand, within Psest_1314 at 1415.070 kb on + strand, within Psest_1314 at 1415.186 kb on + strand, within Psest_1314 at 1415.186 kb on + strand, within Psest_1314 at 1415.186 kb on + strand, within Psest_1314 at 1415.186 kb on + strand, within Psest_1314 at 1415.186 kb on + strand, within Psest_1314 at 1415.187 kb on - strand, within Psest_1314 at 1415.229 kb on + strand, within Psest_1314 at 1415.229 kb on + strand, within Psest_1314 at 1415.230 kb on - strand, within Psest_1314 at 1415.230 kb on - strand, within Psest_1314 at 1415.273 kb on + strand, within Psest_1314 at 1415.288 kb on + strand, within Psest_1314 at 1415.289 kb on - strand, within Psest_1314 at 1415.466 kb on + strand, within Psest_1314 at 1415.563 kb on + strand, within Psest_1314 at 1415.563 kb on + strand, within Psest_1314 at 1415.564 kb on - strand, within Psest_1314 at 1415.564 kb on - strand, within Psest_1314 at 1415.623 kb on + strand, within Psest_1314 at 1415.623 kb on + strand, within Psest_1314 at 1415.623 kb on + strand, within Psest_1314 at 1415.623 kb on + strand, within Psest_1314 at 1415.623 kb on + strand, within Psest_1314 at 1415.623 kb on + strand, within Psest_1314 at 1415.624 kb on - strand, within Psest_1314 at 1415.624 kb on - strand, within Psest_1314 at 1415.624 kb on - strand, within Psest_1314 at 1415.658 kb on - strand, within Psest_1314 at 1415.658 kb on - strand, within Psest_1314 at 1415.658 kb on - strand, within Psest_1314 at 1415.658 kb on - strand, within Psest_1314 at 1415.896 kb on + strand, within Psest_1314 at 1415.896 kb on + strand, within Psest_1314 at 1415.925 kb on - strand, within Psest_1314 at 1415.937 kb on - strand, within Psest_1314 at 1415.963 kb on + strand, within Psest_1314 at 1416.044 kb on + strand, within Psest_1314 at 1416.045 kb on - strand, within Psest_1314 at 1416.045 kb on - strand, within Psest_1314 at 1416.045 kb on - strand, within Psest_1314 at 1416.045 kb on - strand, within Psest_1314 at 1416.045 kb on - strand, within Psest_1314 at 1416.045 kb on - strand, within Psest_1314 at 1416.045 kb on - strand, within Psest_1314 at 1416.061 kb on + strand, within Psest_1314 at 1416.062 kb on - strand, within Psest_1314 at 1416.116 kb on + strand, within Psest_1314 at 1416.116 kb on + strand, within Psest_1314 at 1416.116 kb on + strand, within Psest_1314 at 1416.116 kb on + strand, within Psest_1314 at 1416.161 kb on + strand, within Psest_1314 at 1416.162 kb on - strand, within Psest_1314 at 1416.162 kb on - strand, within Psest_1314 at 1416.189 kb on - strand at 1416.189 kb on - strand at 1416.189 kb on - strand at 1416.289 kb on + strand at 1416.322 kb on + strand at 1416.322 kb on + strand at 1416.322 kb on + strand at 1416.323 kb on - strand at 1416.323 kb on - strand at 1416.323 kb on - strand at 1416.379 kb on + strand, within Psest_1315 at 1416.612 kb on + strand at 1416.613 kb on - strand at 1416.613 kb on - strand at 1416.620 kb on + strand at 1416.639 kb on + strand at 1416.646 kb on + strand at 1416.989 kb on + strand, within Psest_1316 at 1416.989 kb on + strand, within Psest_1316 at 1416.989 kb on + strand, within Psest_1316 at 1416.989 kb on + strand, within Psest_1316 at 1416.989 kb on + strand, within Psest_1316 at 1416.990 kb on - strand, within Psest_1316 at 1416.990 kb on - strand, within Psest_1316 at 1416.990 kb on - strand, within Psest_1316 at 1416.990 kb on - strand, within Psest_1316 at 1416.990 kb on - strand, within Psest_1316 at 1417.002 kb on - strand, within Psest_1316 at 1417.037 kb on + strand, within Psest_1316 at 1417.038 kb on - strand, within Psest_1316 at 1417.038 kb on - strand, within Psest_1316 at 1417.138 kb on + strand, within Psest_1316 at 1417.219 kb on - strand, within Psest_1316
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 0.0001 mg/ml remove 1,413,836 + Psest_1312 0.69 +2.6 1,413,836 + Psest_1312 0.69 -0.4 1,413,837 - Psest_1312 0.69 +0.1 1,413,837 - Psest_1312 0.69 +1.3 1,414,113 + +0.6 1,414,113 + +0.2 1,414,115 + -2.4 1,414,115 + -2.4 1,414,115 + -0.4 1,414,115 + +0.4 1,414,116 - +2.1 1,414,179 + -0.1 1,414,179 + -0.1 1,414,179 + +1.0 1,414,179 + +0.2 1,414,179 + -1.4 1,414,179 + +2.7 1,414,180 - +0.1 1,414,180 - -0.8 1,414,180 - +0.5 1,414,180 - -1.8 1,414,180 - +1.0 1,414,180 - +1.9 1,414,180 - +1.3 1,414,180 - -0.4 1,414,280 + +1.7 1,414,281 - -1.3 1,414,281 - +0.7 1,414,364 + +0.2 1,414,403 + +0.4 1,414,404 - +0.6 1,414,479 + Psest_1313 0.31 +1.8 1,414,479 + Psest_1313 0.31 +0.2 1,414,479 + Psest_1313 0.31 +2.5 1,414,480 - Psest_1313 0.32 -0.8 1,414,480 - Psest_1313 0.32 +1.0 1,414,848 + -0.9 1,414,848 + +1.3 1,414,956 - Psest_1314 0.13 +2.2 1,414,973 + Psest_1314 0.14 +1.8 1,414,973 + Psest_1314 0.14 -2.3 1,414,974 - Psest_1314 0.14 +1.2 1,414,974 - Psest_1314 0.14 +0.6 1,415,070 + Psest_1314 0.20 +0.6 1,415,186 + Psest_1314 0.27 +0.2 1,415,186 + Psest_1314 0.27 +0.3 1,415,186 + Psest_1314 0.27 -1.6 1,415,186 + Psest_1314 0.27 -2.0 1,415,186 + Psest_1314 0.27 +1.7 1,415,187 - Psest_1314 0.27 +0.6 1,415,229 + Psest_1314 0.30 +2.2 1,415,229 + Psest_1314 0.30 +1.0 1,415,230 - Psest_1314 0.30 +2.2 1,415,230 - Psest_1314 0.30 +3.1 1,415,273 + Psest_1314 0.33 -2.3 1,415,288 + Psest_1314 0.34 +1.4 1,415,289 - Psest_1314 0.34 -2.9 1,415,466 + Psest_1314 0.45 -0.4 1,415,563 + Psest_1314 0.51 +0.2 1,415,563 + Psest_1314 0.51 +1.3 1,415,564 - Psest_1314 0.51 +1.5 1,415,564 - Psest_1314 0.51 +1.2 1,415,623 + Psest_1314 0.55 -0.1 1,415,623 + Psest_1314 0.55 -0.1 1,415,623 + Psest_1314 0.55 +1.8 1,415,623 + Psest_1314 0.55 +0.2 1,415,623 + Psest_1314 0.55 -0.4 1,415,623 + Psest_1314 0.55 -0.2 1,415,624 - Psest_1314 0.55 +0.8 1,415,624 - Psest_1314 0.55 -0.4 1,415,624 - Psest_1314 0.55 -1.6 1,415,658 - Psest_1314 0.57 -0.6 1,415,658 - Psest_1314 0.57 -1.6 1,415,658 - Psest_1314 0.57 +0.7 1,415,658 - Psest_1314 0.57 +0.0 1,415,896 + Psest_1314 0.72 +0.7 1,415,896 + Psest_1314 0.72 +1.3 1,415,925 - Psest_1314 0.74 +1.1 1,415,937 - Psest_1314 0.75 +0.2 1,415,963 + Psest_1314 0.76 +0.2 1,416,044 + Psest_1314 0.81 +1.5 1,416,045 - Psest_1314 0.81 +0.9 1,416,045 - Psest_1314 0.81 +0.7 1,416,045 - Psest_1314 0.81 +1.7 1,416,045 - Psest_1314 0.81 +0.2 1,416,045 - Psest_1314 0.81 +0.7 1,416,045 - Psest_1314 0.81 +0.0 1,416,045 - Psest_1314 0.81 +2.6 1,416,061 + Psest_1314 0.82 +4.1 1,416,062 - Psest_1314 0.82 -0.7 1,416,116 + Psest_1314 0.86 -0.4 1,416,116 + Psest_1314 0.86 +2.2 1,416,116 + Psest_1314 0.86 -0.8 1,416,116 + Psest_1314 0.86 -2.0 1,416,161 + Psest_1314 0.89 -1.1 1,416,162 - Psest_1314 0.89 +0.4 1,416,162 - Psest_1314 0.89 -1.6 1,416,189 - +1.5 1,416,189 - +0.2 1,416,189 - +1.6 1,416,289 + +1.5 1,416,322 + -0.4 1,416,322 + +3.2 1,416,322 + +1.7 1,416,323 - -1.6 1,416,323 - -2.6 1,416,323 - +0.5 1,416,379 + Psest_1315 0.17 +1.1 1,416,612 + +0.3 1,416,613 - -2.8 1,416,613 - -2.2 1,416,620 + +0.2 1,416,639 + +2.0 1,416,646 + -0.8 1,416,989 + Psest_1316 0.27 -0.1 1,416,989 + Psest_1316 0.27 -1.4 1,416,989 + Psest_1316 0.27 -0.1 1,416,989 + Psest_1316 0.27 +2.1 1,416,989 + Psest_1316 0.27 +1.0 1,416,990 - Psest_1316 0.27 -1.4 1,416,990 - Psest_1316 0.27 +2.0 1,416,990 - Psest_1316 0.27 +0.2 1,416,990 - Psest_1316 0.27 -0.5 1,416,990 - Psest_1316 0.27 -1.1 1,417,002 - Psest_1316 0.28 -1.8 1,417,037 + Psest_1316 0.30 +1.6 1,417,038 - Psest_1316 0.30 -1.6 1,417,038 - Psest_1316 0.30 +0.2 1,417,138 + Psest_1316 0.38 +3.3 1,417,219 - Psest_1316 0.44 +1.6
Or see this region's nucleotide sequence