Experiment: Spectinomycin 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SOA0167 and SOA0168 are separated by 163 nucleotides SOA0168 and SOA0169 overlap by 4 nucleotides SOA0169 and SOA0170 overlap by 7 nucleotides SOA0170 and SOA0171 are separated by 272 nucleotides SOA0171 and SOA0172 are separated by 0 nucleotides
SOA0167: SOA0167 - ISSod9, transposase (NCBI ptt file), at 155,302 to 158,268
SOA0167
SOA0168: SOA0168 - ISSo9, nucleotidyltransferase domain protein (NCBI ptt file), at 158,432 to 158,722
SOA0168
SOA0169: SOA0169 - ISSod9, conserved hypothetical protein (NCBI ptt file), at 158,719 to 159,096
SOA0169
SOA0170: SOA0170 - ISSod9, DNA-invertase (NCBI ptt file), at 159,090 to 159,647
SOA0170
SOA0171: SOA0171 - hypothetical protein (NCBI ptt file), at 159,920 to 160,177
SOA0171
SOA0172: SOA0172 - site-specific recombinase, resolvase family (NCBI ptt file), at 160,178 to 160,804
SOA0172
Position (kb) 159
160 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 159.687 kb on + strand at 159.699 kb on + strand at 159.700 kb on - strand at 159.700 kb on - strand at 159.709 kb on + strand at 159.709 kb on + strand at 159.709 kb on + strand at 159.709 kb on + strand at 159.778 kb on + strand at 159.778 kb on - strand at 159.783 kb on + strand at 159.791 kb on - strand at 159.791 kb on - strand at 159.813 kb on - strand at 159.840 kb on - strand at 160.098 kb on + strand, within SOA0171 at 160.106 kb on - strand, within SOA0171 at 160.106 kb on - strand, within SOA0171 at 160.125 kb on - strand, within SOA0171 at 160.126 kb on - strand, within SOA0171 at 160.175 kb on - strand at 160.242 kb on + strand, within SOA0172 at 160.291 kb on - strand, within SOA0172 at 160.322 kb on + strand, within SOA0172 at 160.322 kb on - strand, within SOA0172 at 160.383 kb on + strand, within SOA0172 at 160.451 kb on + strand, within SOA0172 at 160.451 kb on + strand, within SOA0172 at 160.459 kb on - strand, within SOA0172 at 160.467 kb on - strand, within SOA0172 at 160.564 kb on + strand, within SOA0172 at 160.564 kb on + strand, within SOA0172 at 160.564 kb on + strand, within SOA0172 at 160.572 kb on - strand, within SOA0172 at 160.572 kb on - strand, within SOA0172 at 160.572 kb on - strand, within SOA0172 at 160.574 kb on + strand, within SOA0172 at 160.582 kb on - strand, within SOA0172
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.025 mg/ml remove 159,687 + -1.7 159,699 + -2.7 159,700 - -2.3 159,700 - -1.6 159,709 + -0.3 159,709 + -2.0 159,709 + -1.8 159,709 + -1.4 159,778 + -0.4 159,778 - -0.7 159,783 + -0.4 159,791 - -2.2 159,791 - -1.8 159,813 - +0.6 159,840 - -2.4 160,098 + SOA0171 0.69 -1.7 160,106 - SOA0171 0.72 -1.0 160,106 - SOA0171 0.72 -2.8 160,125 - SOA0171 0.79 -1.5 160,126 - SOA0171 0.80 -2.8 160,175 - -2.4 160,242 + SOA0172 0.10 -1.3 160,291 - SOA0172 0.18 -1.4 160,322 + SOA0172 0.23 -0.0 160,322 - SOA0172 0.23 -1.4 160,383 + SOA0172 0.33 -0.1 160,451 + SOA0172 0.44 -0.4 160,451 + SOA0172 0.44 -1.4 160,459 - SOA0172 0.45 -0.8 160,467 - SOA0172 0.46 -1.6 160,564 + SOA0172 0.62 -1.4 160,564 + SOA0172 0.62 -0.4 160,564 + SOA0172 0.62 -1.3 160,572 - SOA0172 0.63 -1.3 160,572 - SOA0172 0.63 -2.1 160,572 - SOA0172 0.63 -2.0 160,574 + SOA0172 0.63 -1.4 160,582 - SOA0172 0.64 -2.1
Or see this region's nucleotide sequence