Experiment: m.b. Sisomicin 0.005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c03900 and PGA1_c03910 overlap by 4 nucleotides PGA1_c03910 and PGA1_c03920 are separated by 95 nucleotides PGA1_c03920 and PGA1_c03930 are separated by 1 nucleotides
PGA1_c03900: PGA1_c03900 - hypothetical protein, at 376,259 to 377,797
_c03900
PGA1_c03910: PGA1_c03910 - short chain dehydrogenase / reductase, at 377,794 to 378,552
_c03910
PGA1_c03920: PGA1_c03920 - Putative transmembrane protein (Alph_Pro_TM)., at 378,648 to 379,538
_c03920
PGA1_c03930: PGA1_c03930 - Predicted permeases, at 379,540 to 380,463
_c03930
Position (kb) 378
379
380 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 377.665 kb on + strand at 377.675 kb on + strand at 377.683 kb on - strand at 377.726 kb on - strand at 377.747 kb on + strand at 377.747 kb on + strand at 377.755 kb on - strand at 377.767 kb on - strand at 377.767 kb on - strand at 377.798 kb on + strand at 377.813 kb on - strand at 377.835 kb on - strand at 377.918 kb on + strand, within PGA1_c03910 at 377.926 kb on - strand, within PGA1_c03910 at 377.927 kb on + strand, within PGA1_c03910 at 377.935 kb on - strand, within PGA1_c03910 at 377.959 kb on + strand, within PGA1_c03910 at 377.988 kb on + strand, within PGA1_c03910 at 377.988 kb on + strand, within PGA1_c03910 at 377.988 kb on + strand, within PGA1_c03910 at 377.996 kb on - strand, within PGA1_c03910 at 378.001 kb on + strand, within PGA1_c03910 at 378.037 kb on - strand, within PGA1_c03910 at 378.086 kb on + strand, within PGA1_c03910 at 378.210 kb on - strand, within PGA1_c03910 at 378.254 kb on - strand, within PGA1_c03910 at 378.304 kb on - strand, within PGA1_c03910 at 378.304 kb on - strand, within PGA1_c03910 at 378.304 kb on - strand, within PGA1_c03910 at 378.304 kb on - strand, within PGA1_c03910 at 378.304 kb on - strand, within PGA1_c03910 at 378.306 kb on + strand, within PGA1_c03910 at 378.316 kb on - strand, within PGA1_c03910 at 378.399 kb on + strand, within PGA1_c03910 at 378.399 kb on + strand, within PGA1_c03910 at 378.399 kb on + strand, within PGA1_c03910 at 378.407 kb on - strand, within PGA1_c03910 at 378.407 kb on - strand, within PGA1_c03910 at 378.407 kb on - strand, within PGA1_c03910 at 378.427 kb on + strand, within PGA1_c03910 at 378.452 kb on - strand, within PGA1_c03910 at 378.481 kb on - strand at 378.626 kb on + strand at 378.644 kb on - strand at 378.725 kb on - strand at 378.773 kb on + strand, within PGA1_c03920 at 378.773 kb on + strand, within PGA1_c03920 at 378.933 kb on - strand, within PGA1_c03920 at 379.089 kb on - strand, within PGA1_c03920 at 379.144 kb on - strand, within PGA1_c03920 at 379.157 kb on + strand, within PGA1_c03920 at 379.237 kb on + strand, within PGA1_c03920 at 379.482 kb on + strand at 379.482 kb on + strand at 379.490 kb on - strand at 379.615 kb on + strand at 379.626 kb on + strand at 379.634 kb on - strand, within PGA1_c03930 at 379.750 kb on + strand, within PGA1_c03930 at 379.826 kb on - strand, within PGA1_c03930 at 379.929 kb on - strand, within PGA1_c03930 at 379.934 kb on - strand, within PGA1_c03930 at 379.963 kb on - strand, within PGA1_c03930 at 380.125 kb on - strand, within PGA1_c03930 at 380.234 kb on - strand, within PGA1_c03930 at 380.468 kb on + strand at 380.468 kb on + strand at 380.476 kb on - strand at 380.476 kb on - strand at 380.476 kb on - strand at 380.476 kb on - strand at 380.476 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Sisomicin 0.005 mg/ml remove 377,665 + +0.2 377,675 + -0.8 377,683 - -1.8 377,726 - +0.4 377,747 + -1.6 377,747 + -1.3 377,755 - -1.0 377,767 - -0.1 377,767 - +0.1 377,798 + -0.1 377,813 - -2.7 377,835 - -0.4 377,918 + PGA1_c03910 0.16 -1.0 377,926 - PGA1_c03910 0.17 +2.2 377,927 + PGA1_c03910 0.18 -1.6 377,935 - PGA1_c03910 0.19 -0.8 377,959 + PGA1_c03910 0.22 -0.1 377,988 + PGA1_c03910 0.26 +0.3 377,988 + PGA1_c03910 0.26 -0.2 377,988 + PGA1_c03910 0.26 -0.8 377,996 - PGA1_c03910 0.27 -0.4 378,001 + PGA1_c03910 0.27 -1.2 378,037 - PGA1_c03910 0.32 +1.0 378,086 + PGA1_c03910 0.38 +0.3 378,210 - PGA1_c03910 0.55 -0.2 378,254 - PGA1_c03910 0.61 -1.8 378,304 - PGA1_c03910 0.67 +1.0 378,304 - PGA1_c03910 0.67 -0.2 378,304 - PGA1_c03910 0.67 +0.2 378,304 - PGA1_c03910 0.67 -0.9 378,304 - PGA1_c03910 0.67 -2.9 378,306 + PGA1_c03910 0.67 +0.2 378,316 - PGA1_c03910 0.69 -0.2 378,399 + PGA1_c03910 0.80 +1.2 378,399 + PGA1_c03910 0.80 -0.1 378,399 + PGA1_c03910 0.80 -2.2 378,407 - PGA1_c03910 0.81 -0.4 378,407 - PGA1_c03910 0.81 +0.6 378,407 - PGA1_c03910 0.81 -0.8 378,427 + PGA1_c03910 0.83 -0.8 378,452 - PGA1_c03910 0.87 -1.3 378,481 - -0.8 378,626 + -1.0 378,644 - +0.1 378,725 - -2.4 378,773 + PGA1_c03920 0.14 -3.4 378,773 + PGA1_c03920 0.14 -1.2 378,933 - PGA1_c03920 0.32 -0.6 379,089 - PGA1_c03920 0.49 -1.9 379,144 - PGA1_c03920 0.56 -3.9 379,157 + PGA1_c03920 0.57 -0.8 379,237 + PGA1_c03920 0.66 -3.4 379,482 + -2.5 379,482 + -1.8 379,490 - -3.4 379,615 + -1.8 379,626 + -1.6 379,634 - PGA1_c03930 0.10 -2.0 379,750 + PGA1_c03930 0.23 -2.7 379,826 - PGA1_c03930 0.31 -2.4 379,929 - PGA1_c03930 0.42 +0.2 379,934 - PGA1_c03930 0.43 -1.0 379,963 - PGA1_c03930 0.46 -2.7 380,125 - PGA1_c03930 0.63 -2.7 380,234 - PGA1_c03930 0.75 -3.2 380,468 + -1.6 380,468 + +0.5 380,476 - +0.3 380,476 - -1.8 380,476 - +0.8 380,476 - +0.5 380,476 - -0.4
Or see this region's nucleotide sequence