Experiment: NLDM_defined_nocarbon with D-Glucose and L-Methionine 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt leuC and leuD are separated by 226 nucleotides leuD and leuB overlap by 4 nucleotides leuB and ilvD are separated by 270 nucleotides
OKGIIK_13585: leuC - 3-isopropylmalate dehydratase large subunit, at 2,944,871 to 2,946,283
leuC
OKGIIK_13590: leuD - 3-isopropylmalate dehydratase small subunit, at 2,946,510 to 2,947,154
leuD
OKGIIK_13595: leuB - 3-isopropylmalate dehydrogenase, at 2,947,151 to 2,948,272
leuB
OKGIIK_13600: ilvD - dihydroxy-acid dehydratase, at 2,948,543 to 2,950,249
ilvD
Position (kb)
2947
2948
2949 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1
2 at 2946.260 kb on + strand at 2946.261 kb on - strand at 2946.261 kb on - strand at 2946.261 kb on - strand at 2946.369 kb on - strand at 2946.369 kb on - strand at 2946.786 kb on + strand, within leuD at 2946.786 kb on + strand, within leuD at 2946.786 kb on + strand, within leuD at 2947.032 kb on + strand, within leuD at 2947.032 kb on + strand, within leuD at 2947.033 kb on - strand, within leuD at 2947.144 kb on + strand at 2947.144 kb on + strand at 2947.145 kb on - strand at 2947.145 kb on - strand at 2947.145 kb on - strand at 2947.168 kb on + strand at 2947.228 kb on + strand at 2947.228 kb on + strand at 2947.229 kb on - strand at 2947.229 kb on - strand at 2947.245 kb on - strand at 2947.454 kb on + strand, within leuB at 2947.454 kb on + strand, within leuB at 2947.454 kb on + strand, within leuB at 2947.454 kb on + strand, within leuB at 2947.454 kb on + strand, within leuB at 2947.455 kb on - strand, within leuB at 2947.571 kb on + strand, within leuB at 2947.571 kb on + strand, within leuB at 2947.571 kb on + strand, within leuB at 2947.571 kb on + strand, within leuB at 2947.571 kb on + strand, within leuB at 2947.571 kb on + strand, within leuB at 2947.572 kb on - strand, within leuB at 2947.572 kb on - strand, within leuB at 2947.572 kb on - strand, within leuB at 2947.572 kb on - strand, within leuB at 2947.572 kb on - strand, within leuB at 2947.572 kb on - strand, within leuB at 2947.572 kb on - strand, within leuB at 2947.622 kb on + strand, within leuB at 2947.622 kb on + strand, within leuB at 2947.622 kb on + strand, within leuB at 2947.622 kb on + strand, within leuB at 2947.622 kb on + strand, within leuB at 2947.622 kb on + strand, within leuB at 2947.622 kb on + strand, within leuB at 2947.622 kb on + strand, within leuB at 2947.623 kb on - strand, within leuB at 2947.623 kb on - strand, within leuB at 2947.623 kb on - strand, within leuB at 2947.623 kb on - strand, within leuB at 2947.623 kb on - strand, within leuB at 2947.623 kb on - strand, within leuB at 2947.623 kb on - strand, within leuB at 2947.769 kb on + strand, within leuB at 2947.770 kb on - strand, within leuB at 2947.770 kb on - strand, within leuB at 2947.841 kb on + strand, within leuB at 2947.841 kb on + strand, within leuB at 2947.841 kb on + strand, within leuB at 2947.841 kb on + strand, within leuB at 2947.841 kb on + strand, within leuB at 2947.841 kb on + strand, within leuB at 2947.841 kb on + strand, within leuB at 2947.841 kb on + strand, within leuB at 2947.841 kb on + strand, within leuB at 2947.841 kb on + strand, within leuB at 2947.841 kb on + strand, within leuB at 2947.842 kb on - strand, within leuB at 2947.842 kb on - strand, within leuB at 2947.842 kb on - strand, within leuB at 2947.842 kb on - strand, within leuB at 2947.842 kb on - strand, within leuB at 2947.842 kb on - strand, within leuB at 2947.842 kb on - strand, within leuB at 2947.842 kb on - strand, within leuB at 2947.957 kb on + strand, within leuB at 2947.957 kb on + strand, within leuB at 2947.958 kb on - strand, within leuB at 2947.983 kb on + strand, within leuB at 2947.983 kb on + strand, within leuB at 2947.983 kb on + strand, within leuB at 2947.984 kb on - strand, within leuB at 2947.984 kb on - strand, within leuB at 2947.984 kb on - strand, within leuB at 2947.985 kb on + strand, within leuB at 2947.985 kb on + strand, within leuB at 2947.985 kb on + strand, within leuB at 2947.985 kb on + strand, within leuB at 2947.985 kb on + strand, within leuB at 2947.985 kb on + strand, within leuB at 2947.985 kb on + strand, within leuB at 2947.985 kb on + strand, within leuB at 2947.985 kb on + strand, within leuB at 2947.985 kb on + strand, within leuB at 2947.985 kb on + strand, within leuB at 2947.985 kb on + strand, within leuB at 2947.985 kb on + strand, within leuB at 2947.985 kb on + strand, within leuB at 2947.986 kb on - strand, within leuB at 2947.986 kb on - strand, within leuB at 2947.986 kb on - strand, within leuB at 2947.986 kb on - strand, within leuB at 2947.986 kb on - strand, within leuB at 2947.986 kb on - strand, within leuB at 2947.986 kb on - strand, within leuB at 2947.986 kb on - strand, within leuB at 2947.986 kb on - strand, within leuB at 2947.986 kb on - strand, within leuB at 2947.986 kb on - strand, within leuB at 2947.986 kb on - strand, within leuB at 2947.986 kb on - strand, within leuB at 2947.986 kb on - strand, within leuB at 2947.986 kb on - strand, within leuB at 2947.986 kb on - strand, within leuB at 2947.986 kb on - strand, within leuB at 2948.070 kb on + strand, within leuB at 2948.070 kb on + strand, within leuB at 2948.070 kb on + strand, within leuB at 2948.070 kb on + strand, within leuB at 2948.070 kb on + strand, within leuB at 2948.070 kb on + strand, within leuB at 2948.071 kb on - strand, within leuB at 2948.071 kb on - strand, within leuB at 2948.071 kb on - strand, within leuB at 2948.071 kb on - strand, within leuB at 2948.071 kb on - strand, within leuB at 2948.071 kb on - strand, within leuB at 2948.071 kb on - strand, within leuB at 2948.133 kb on + strand, within leuB at 2948.133 kb on + strand, within leuB at 2948.133 kb on + strand, within leuB at 2948.133 kb on + strand, within leuB at 2948.133 kb on + strand, within leuB at 2948.133 kb on + strand, within leuB at 2948.133 kb on + strand, within leuB at 2948.133 kb on + strand, within leuB at 2948.133 kb on + strand, within leuB at 2948.133 kb on + strand, within leuB at 2948.133 kb on + strand, within leuB at 2948.133 kb on + strand, within leuB at 2948.133 kb on + strand, within leuB at 2948.133 kb on + strand, within leuB at 2948.133 kb on + strand, within leuB at 2948.133 kb on + strand, within leuB at 2948.133 kb on + strand, within leuB at 2948.133 kb on + strand, within leuB at 2948.133 kb on + strand, within leuB at 2948.134 kb on - strand, within leuB at 2948.134 kb on - strand, within leuB at 2948.134 kb on - strand, within leuB at 2948.134 kb on - strand, within leuB at 2948.134 kb on - strand, within leuB at 2948.134 kb on - strand, within leuB at 2948.134 kb on - strand, within leuB at 2948.134 kb on - strand, within leuB at 2948.134 kb on - strand, within leuB at 2948.134 kb on - strand, within leuB at 2948.134 kb on - strand, within leuB at 2948.134 kb on - strand, within leuB at 2948.134 kb on - strand, within leuB at 2948.134 kb on - strand, within leuB at 2948.134 kb on - strand, within leuB at 2948.134 kb on - strand, within leuB at 2948.134 kb on - strand, within leuB at 2948.134 kb on - strand, within leuB at 2948.134 kb on - strand, within leuB at 2948.278 kb on - strand at 2948.963 kb on + strand, within ilvD at 2949.021 kb on + strand, within ilvD at 2949.021 kb on + strand, within ilvD at 2949.021 kb on + strand, within ilvD at 2949.021 kb on + strand, within ilvD at 2949.021 kb on + strand, within ilvD at 2949.021 kb on + strand, within ilvD at 2949.021 kb on + strand, within ilvD at 2949.022 kb on - strand, within ilvD at 2949.022 kb on - strand, within ilvD at 2949.022 kb on - strand, within ilvD at 2949.022 kb on - strand, within ilvD at 2949.022 kb on - strand, within ilvD at 2949.022 kb on - strand, within ilvD at 2949.022 kb on - strand, within ilvD at 2949.022 kb on - strand, within ilvD at 2949.182 kb on + strand, within ilvD at 2949.182 kb on + strand, within ilvD at 2949.182 kb on + strand, within ilvD at 2949.183 kb on - strand, within ilvD at 2949.183 kb on - strand, within ilvD at 2949.183 kb on - strand, within ilvD at 2949.183 kb on - strand, within ilvD
Per-strain Table
Position Strand Gene LocusTag Fraction NLDM_defined_nocarbon with D-Glucose and L-Methionine 1 mM remove 2,946,260 + -0.8 2,946,261 - -1.3 2,946,261 - -0.6 2,946,261 - -3.5 2,946,369 - +1.0 2,946,369 - -0.6 2,946,786 + leuD OKGIIK_13590 0.43 -2.2 2,946,786 + leuD OKGIIK_13590 0.43 -2.0 2,946,786 + leuD OKGIIK_13590 0.43 -2.2 2,947,032 + leuD OKGIIK_13590 0.81 -2.5 2,947,032 + leuD OKGIIK_13590 0.81 -1.3 2,947,033 - leuD OKGIIK_13590 0.81 -3.6 2,947,144 + -1.0 2,947,144 + -0.3 2,947,145 - -1.3 2,947,145 - +0.0 2,947,145 - -2.6 2,947,168 + -1.0 2,947,228 + -2.0 2,947,228 + -3.0 2,947,229 - -3.3 2,947,229 - -1.8 2,947,245 - -1.8 2,947,454 + leuB OKGIIK_13595 0.27 +0.0 2,947,454 + leuB OKGIIK_13595 0.27 -6.1 2,947,454 + leuB OKGIIK_13595 0.27 -1.0 2,947,454 + leuB OKGIIK_13595 0.27 +0.0 2,947,454 + leuB OKGIIK_13595 0.27 -3.4 2,947,455 - leuB OKGIIK_13595 0.27 -1.0 2,947,571 + leuB OKGIIK_13595 0.37 -1.8 2,947,571 + leuB OKGIIK_13595 0.37 -3.7 2,947,571 + leuB OKGIIK_13595 0.37 -1.0 2,947,571 + leuB OKGIIK_13595 0.37 -3.2 2,947,571 + leuB OKGIIK_13595 0.37 -2.1 2,947,571 + leuB OKGIIK_13595 0.37 +1.6 2,947,572 - leuB OKGIIK_13595 0.38 -3.2 2,947,572 - leuB OKGIIK_13595 0.38 -2.7 2,947,572 - leuB OKGIIK_13595 0.38 -3.2 2,947,572 - leuB OKGIIK_13595 0.38 +0.0 2,947,572 - leuB OKGIIK_13595 0.38 -2.7 2,947,572 - leuB OKGIIK_13595 0.38 -1.3 2,947,572 - leuB OKGIIK_13595 0.38 -2.0 2,947,622 + leuB OKGIIK_13595 0.42 -0.3 2,947,622 + leuB OKGIIK_13595 0.42 -2.6 2,947,622 + leuB OKGIIK_13595 0.42 -3.4 2,947,622 + leuB OKGIIK_13595 0.42 -2.6 2,947,622 + leuB OKGIIK_13595 0.42 -3.7 2,947,622 + leuB OKGIIK_13595 0.42 -1.6 2,947,622 + leuB OKGIIK_13595 0.42 -1.0 2,947,622 + leuB OKGIIK_13595 0.42 +0.0 2,947,623 - leuB OKGIIK_13595 0.42 -1.5 2,947,623 - leuB OKGIIK_13595 0.42 -2.8 2,947,623 - leuB OKGIIK_13595 0.42 -1.3 2,947,623 - leuB OKGIIK_13595 0.42 -2.8 2,947,623 - leuB OKGIIK_13595 0.42 -5.1 2,947,623 - leuB OKGIIK_13595 0.42 -1.2 2,947,623 - leuB OKGIIK_13595 0.42 -2.3 2,947,769 + leuB OKGIIK_13595 0.55 +0.2 2,947,770 - leuB OKGIIK_13595 0.55 -1.6 2,947,770 - leuB OKGIIK_13595 0.55 -2.0 2,947,841 + leuB OKGIIK_13595 0.61 -0.6 2,947,841 + leuB OKGIIK_13595 0.61 -1.6 2,947,841 + leuB OKGIIK_13595 0.61 -2.2 2,947,841 + leuB OKGIIK_13595 0.61 -1.0 2,947,841 + leuB OKGIIK_13595 0.61 -2.5 2,947,841 + leuB OKGIIK_13595 0.61 -1.8 2,947,841 + leuB OKGIIK_13595 0.61 -1.0 2,947,841 + leuB OKGIIK_13595 0.61 -2.2 2,947,841 + leuB OKGIIK_13595 0.61 -2.0 2,947,841 + leuB OKGIIK_13595 0.61 -1.6 2,947,841 + leuB OKGIIK_13595 0.61 -2.5 2,947,842 - leuB OKGIIK_13595 0.62 -2.1 2,947,842 - leuB OKGIIK_13595 0.62 -0.6 2,947,842 - leuB OKGIIK_13595 0.62 -1.0 2,947,842 - leuB OKGIIK_13595 0.62 -1.3 2,947,842 - leuB OKGIIK_13595 0.62 -1.3 2,947,842 - leuB OKGIIK_13595 0.62 -2.6 2,947,842 - leuB OKGIIK_13595 0.62 -1.0 2,947,842 - leuB OKGIIK_13595 0.62 -2.2 2,947,957 + leuB OKGIIK_13595 0.72 -2.3 2,947,957 + leuB OKGIIK_13595 0.72 -4.0 2,947,958 - leuB OKGIIK_13595 0.72 -1.8 2,947,983 + leuB OKGIIK_13595 0.74 -1.8 2,947,983 + leuB OKGIIK_13595 0.74 -3.0 2,947,983 + leuB OKGIIK_13595 0.74 +0.0 2,947,984 - leuB OKGIIK_13595 0.74 -2.1 2,947,984 - leuB OKGIIK_13595 0.74 +0.0 2,947,984 - leuB OKGIIK_13595 0.74 -2.5 2,947,985 + leuB OKGIIK_13595 0.74 -3.5 2,947,985 + leuB OKGIIK_13595 0.74 -1.3 2,947,985 + leuB OKGIIK_13595 0.74 -2.5 2,947,985 + leuB OKGIIK_13595 0.74 -2.9 2,947,985 + leuB OKGIIK_13595 0.74 +1.0 2,947,985 + leuB OKGIIK_13595 0.74 -1.8 2,947,985 + leuB OKGIIK_13595 0.74 +1.0 2,947,985 + leuB OKGIIK_13595 0.74 -4.3 2,947,985 + leuB OKGIIK_13595 0.74 -2.0 2,947,985 + leuB OKGIIK_13595 0.74 -0.2 2,947,985 + leuB OKGIIK_13595 0.74 -1.0 2,947,985 + leuB OKGIIK_13595 0.74 -2.8 2,947,985 + leuB OKGIIK_13595 0.74 -0.6 2,947,985 + leuB OKGIIK_13595 0.74 -1.6 2,947,986 - leuB OKGIIK_13595 0.74 -2.9 2,947,986 - leuB OKGIIK_13595 0.74 -2.6 2,947,986 - leuB OKGIIK_13595 0.74 -1.6 2,947,986 - leuB OKGIIK_13595 0.74 -2.5 2,947,986 - leuB OKGIIK_13595 0.74 -1.8 2,947,986 - leuB OKGIIK_13595 0.74 -1.3 2,947,986 - leuB OKGIIK_13595 0.74 -2.0 2,947,986 - leuB OKGIIK_13595 0.74 -3.0 2,947,986 - leuB OKGIIK_13595 0.74 -0.6 2,947,986 - leuB OKGIIK_13595 0.74 -0.6 2,947,986 - leuB OKGIIK_13595 0.74 +0.4 2,947,986 - leuB OKGIIK_13595 0.74 -0.6 2,947,986 - leuB OKGIIK_13595 0.74 +1.0 2,947,986 - leuB OKGIIK_13595 0.74 -3.0 2,947,986 - leuB OKGIIK_13595 0.74 -1.3 2,947,986 - leuB OKGIIK_13595 0.74 -1.0 2,947,986 - leuB OKGIIK_13595 0.74 -2.0 2,948,070 + leuB OKGIIK_13595 0.82 -3.9 2,948,070 + leuB OKGIIK_13595 0.82 -1.0 2,948,070 + leuB OKGIIK_13595 0.82 -2.2 2,948,070 + leuB OKGIIK_13595 0.82 -2.0 2,948,070 + leuB OKGIIK_13595 0.82 -2.2 2,948,070 + leuB OKGIIK_13595 0.82 -0.6 2,948,071 - leuB OKGIIK_13595 0.82 -0.6 2,948,071 - leuB OKGIIK_13595 0.82 -1.8 2,948,071 - leuB OKGIIK_13595 0.82 -2.8 2,948,071 - leuB OKGIIK_13595 0.82 -2.3 2,948,071 - leuB OKGIIK_13595 0.82 -1.3 2,948,071 - leuB OKGIIK_13595 0.82 +1.0 2,948,071 - leuB OKGIIK_13595 0.82 -2.9 2,948,133 + leuB OKGIIK_13595 0.88 -4.3 2,948,133 + leuB OKGIIK_13595 0.88 -2.5 2,948,133 + leuB OKGIIK_13595 0.88 -0.6 2,948,133 + leuB OKGIIK_13595 0.88 -2.7 2,948,133 + leuB OKGIIK_13595 0.88 -2.1 2,948,133 + leuB OKGIIK_13595 0.88 -1.3 2,948,133 + leuB OKGIIK_13595 0.88 -1.6 2,948,133 + leuB OKGIIK_13595 0.88 -2.2 2,948,133 + leuB OKGIIK_13595 0.88 -0.6 2,948,133 + leuB OKGIIK_13595 0.88 -2.8 2,948,133 + leuB OKGIIK_13595 0.88 -1.2 2,948,133 + leuB OKGIIK_13595 0.88 -2.9 2,948,133 + leuB OKGIIK_13595 0.88 -2.4 2,948,133 + leuB OKGIIK_13595 0.88 -2.8 2,948,133 + leuB OKGIIK_13595 0.88 +0.0 2,948,133 + leuB OKGIIK_13595 0.88 -1.3 2,948,133 + leuB OKGIIK_13595 0.88 -1.6 2,948,133 + leuB OKGIIK_13595 0.88 +2.0 2,948,133 + leuB OKGIIK_13595 0.88 -1.2 2,948,134 - leuB OKGIIK_13595 0.88 -5.7 2,948,134 - leuB OKGIIK_13595 0.88 -3.0 2,948,134 - leuB OKGIIK_13595 0.88 -2.2 2,948,134 - leuB OKGIIK_13595 0.88 -1.6 2,948,134 - leuB OKGIIK_13595 0.88 -3.1 2,948,134 - leuB OKGIIK_13595 0.88 -1.0 2,948,134 - leuB OKGIIK_13595 0.88 +0.0 2,948,134 - leuB OKGIIK_13595 0.88 -2.9 2,948,134 - leuB OKGIIK_13595 0.88 -0.6 2,948,134 - leuB OKGIIK_13595 0.88 -1.3 2,948,134 - leuB OKGIIK_13595 0.88 -1.3 2,948,134 - leuB OKGIIK_13595 0.88 -2.2 2,948,134 - leuB OKGIIK_13595 0.88 -2.3 2,948,134 - leuB OKGIIK_13595 0.88 +2.0 2,948,134 - leuB OKGIIK_13595 0.88 -1.0 2,948,134 - leuB OKGIIK_13595 0.88 -0.6 2,948,134 - leuB OKGIIK_13595 0.88 -3.5 2,948,134 - leuB OKGIIK_13595 0.88 +1.0 2,948,134 - leuB OKGIIK_13595 0.88 -2.6 2,948,278 - -0.6 2,948,963 + ilvD OKGIIK_13600 0.25 -1.8 2,949,021 + ilvD OKGIIK_13600 0.28 -2.0 2,949,021 + ilvD OKGIIK_13600 0.28 -1.0 2,949,021 + ilvD OKGIIK_13600 0.28 -2.8 2,949,021 + ilvD OKGIIK_13600 0.28 -1.0 2,949,021 + ilvD OKGIIK_13600 0.28 -2.9 2,949,021 + ilvD OKGIIK_13600 0.28 -2.0 2,949,021 + ilvD OKGIIK_13600 0.28 -0.6 2,949,022 - ilvD OKGIIK_13600 0.28 -3.0 2,949,022 - ilvD OKGIIK_13600 0.28 -2.4 2,949,022 - ilvD OKGIIK_13600 0.28 -2.3 2,949,022 - ilvD OKGIIK_13600 0.28 -3.2 2,949,022 - ilvD OKGIIK_13600 0.28 -0.3 2,949,022 - ilvD OKGIIK_13600 0.28 -2.6 2,949,022 - ilvD OKGIIK_13600 0.28 +0.0 2,949,022 - ilvD OKGIIK_13600 0.28 +1.0 2,949,182 + ilvD OKGIIK_13600 0.37 -1.3 2,949,182 + ilvD OKGIIK_13600 0.37 -3.4 2,949,182 + ilvD OKGIIK_13600 0.37 -1.6 2,949,183 - ilvD OKGIIK_13600 0.37 -1.8 2,949,183 - ilvD OKGIIK_13600 0.37 -3.0 2,949,183 - ilvD OKGIIK_13600 0.37 -1.3 2,949,183 - ilvD OKGIIK_13600 0.37 -1.6
Or see this region's nucleotide sequence