Experiment: NLDM_defined_nocarbon with D-Glucose and L-Methionine 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt leuA and ilvE overlap by 4 nucleotides ilvE and leuC overlap by 4 nucleotides
OKGIIK_13575: leuA - 2-isopropylmalate synthase, at 2,942,221 to 2,943,876
leuA
OKGIIK_13580: ilvE - branched-chain amino acid transaminase, at 2,943,873 to 2,944,874
ilvE
OKGIIK_13585: leuC - 3-isopropylmalate dehydratase large subunit, at 2,944,871 to 2,946,283
leuC
Position (kb)
2943
2944
2945 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 2943.067 kb on + strand, within leuA at 2943.067 kb on + strand, within leuA at 2943.068 kb on - strand, within leuA at 2943.199 kb on + strand, within leuA at 2943.199 kb on + strand, within leuA at 2943.199 kb on + strand, within leuA at 2943.200 kb on - strand, within leuA at 2943.200 kb on - strand, within leuA at 2943.200 kb on - strand, within leuA at 2943.200 kb on - strand, within leuA at 2943.200 kb on - strand, within leuA at 2943.200 kb on - strand, within leuA at 2943.200 kb on - strand, within leuA at 2943.958 kb on - strand at 2943.958 kb on - strand at 2943.958 kb on - strand at 2943.990 kb on + strand, within ilvE at 2943.990 kb on + strand, within ilvE at 2943.990 kb on + strand, within ilvE at 2943.990 kb on + strand, within ilvE at 2943.990 kb on + strand, within ilvE at 2943.990 kb on + strand, within ilvE at 2943.990 kb on + strand, within ilvE at 2943.990 kb on + strand, within ilvE at 2943.990 kb on + strand, within ilvE at 2943.990 kb on + strand, within ilvE at 2943.990 kb on + strand, within ilvE at 2943.990 kb on + strand, within ilvE at 2943.991 kb on - strand, within ilvE at 2943.991 kb on - strand, within ilvE at 2943.991 kb on - strand, within ilvE at 2943.991 kb on - strand, within ilvE at 2943.991 kb on - strand, within ilvE at 2943.991 kb on - strand, within ilvE at 2943.991 kb on - strand, within ilvE at 2944.014 kb on + strand, within ilvE at 2944.014 kb on + strand, within ilvE at 2944.014 kb on + strand, within ilvE at 2944.014 kb on + strand, within ilvE at 2944.015 kb on - strand, within ilvE at 2944.015 kb on - strand, within ilvE at 2944.015 kb on - strand, within ilvE at 2944.065 kb on + strand, within ilvE at 2944.066 kb on - strand, within ilvE at 2944.066 kb on - strand, within ilvE at 2944.149 kb on + strand, within ilvE at 2944.149 kb on + strand, within ilvE at 2944.149 kb on + strand, within ilvE at 2944.149 kb on + strand, within ilvE at 2944.149 kb on + strand, within ilvE at 2944.149 kb on + strand, within ilvE at 2944.149 kb on + strand, within ilvE at 2944.149 kb on + strand, within ilvE at 2944.149 kb on + strand, within ilvE at 2944.149 kb on + strand, within ilvE at 2944.149 kb on + strand, within ilvE at 2944.149 kb on + strand, within ilvE at 2944.149 kb on + strand, within ilvE at 2944.150 kb on - strand, within ilvE at 2944.150 kb on - strand, within ilvE at 2944.150 kb on - strand, within ilvE at 2944.150 kb on - strand, within ilvE at 2944.150 kb on - strand, within ilvE at 2944.150 kb on - strand, within ilvE at 2944.150 kb on - strand, within ilvE at 2944.150 kb on - strand, within ilvE at 2944.150 kb on - strand, within ilvE at 2944.150 kb on - strand, within ilvE at 2944.150 kb on - strand, within ilvE at 2944.150 kb on - strand, within ilvE at 2944.251 kb on + strand, within ilvE at 2944.251 kb on + strand, within ilvE at 2944.251 kb on + strand, within ilvE at 2944.251 kb on + strand, within ilvE at 2944.251 kb on + strand, within ilvE at 2944.251 kb on + strand, within ilvE at 2944.251 kb on + strand, within ilvE at 2944.251 kb on + strand, within ilvE at 2944.251 kb on + strand, within ilvE at 2944.252 kb on - strand, within ilvE at 2944.252 kb on - strand, within ilvE at 2944.252 kb on - strand, within ilvE at 2944.353 kb on + strand, within ilvE at 2944.353 kb on + strand, within ilvE at 2944.353 kb on + strand, within ilvE at 2944.353 kb on + strand, within ilvE at 2944.353 kb on + strand, within ilvE at 2944.353 kb on + strand, within ilvE at 2944.353 kb on + strand, within ilvE at 2944.353 kb on + strand, within ilvE at 2944.353 kb on + strand, within ilvE at 2944.353 kb on + strand, within ilvE at 2944.353 kb on + strand, within ilvE at 2944.353 kb on + strand, within ilvE at 2944.353 kb on + strand, within ilvE at 2944.353 kb on + strand, within ilvE at 2944.353 kb on + strand, within ilvE at 2944.353 kb on + strand, within ilvE at 2944.353 kb on + strand, within ilvE at 2944.354 kb on - strand, within ilvE at 2944.354 kb on - strand, within ilvE at 2944.354 kb on - strand, within ilvE at 2944.354 kb on - strand, within ilvE at 2944.354 kb on - strand, within ilvE at 2944.354 kb on - strand, within ilvE at 2944.354 kb on - strand, within ilvE at 2944.354 kb on - strand, within ilvE at 2944.354 kb on - strand, within ilvE at 2944.354 kb on - strand, within ilvE at 2944.354 kb on - strand, within ilvE at 2944.354 kb on - strand, within ilvE at 2944.354 kb on - strand, within ilvE at 2944.354 kb on - strand, within ilvE at 2944.354 kb on - strand, within ilvE at 2944.354 kb on - strand, within ilvE at 2944.602 kb on + strand, within ilvE at 2944.602 kb on + strand, within ilvE at 2944.603 kb on - strand, within ilvE at 2944.603 kb on - strand, within ilvE at 2944.603 kb on - strand, within ilvE at 2944.603 kb on - strand, within ilvE at 2944.748 kb on + strand, within ilvE at 2944.781 kb on + strand at 2944.952 kb on + strand at 2944.952 kb on + strand at 2944.952 kb on + strand at 2944.952 kb on + strand at 2944.952 kb on + strand at 2944.952 kb on + strand at 2944.952 kb on + strand at 2944.952 kb on + strand at 2944.953 kb on - strand at 2944.953 kb on - strand at 2944.953 kb on - strand at 2944.953 kb on - strand at 2945.109 kb on - strand, within leuC at 2945.446 kb on + strand, within leuC at 2945.446 kb on + strand, within leuC at 2945.446 kb on + strand, within leuC at 2945.446 kb on + strand, within leuC at 2945.446 kb on + strand, within leuC at 2945.446 kb on + strand, within leuC at 2945.446 kb on + strand, within leuC at 2945.446 kb on + strand, within leuC at 2945.446 kb on + strand, within leuC at 2945.446 kb on + strand, within leuC at 2945.446 kb on + strand, within leuC at 2945.446 kb on + strand, within leuC at 2945.446 kb on + strand, within leuC at 2945.447 kb on - strand, within leuC at 2945.447 kb on - strand, within leuC at 2945.447 kb on - strand, within leuC at 2945.447 kb on - strand, within leuC at 2945.447 kb on - strand, within leuC at 2945.447 kb on - strand, within leuC at 2945.495 kb on + strand, within leuC at 2945.495 kb on + strand, within leuC at 2945.705 kb on + strand, within leuC at 2945.706 kb on - strand, within leuC at 2945.756 kb on + strand, within leuC at 2945.756 kb on + strand, within leuC at 2945.756 kb on + strand, within leuC at 2945.756 kb on + strand, within leuC at 2945.756 kb on + strand, within leuC at 2945.757 kb on - strand, within leuC at 2945.757 kb on - strand, within leuC at 2945.757 kb on - strand, within leuC at 2945.757 kb on - strand, within leuC at 2945.758 kb on - strand, within leuC at 2945.840 kb on + strand, within leuC at 2945.840 kb on + strand, within leuC at 2945.840 kb on + strand, within leuC at 2945.840 kb on + strand, within leuC at 2945.840 kb on + strand, within leuC at 2945.840 kb on + strand, within leuC at 2945.840 kb on + strand, within leuC at 2945.840 kb on + strand, within leuC at 2945.840 kb on + strand, within leuC at 2945.840 kb on + strand, within leuC at 2945.841 kb on - strand, within leuC at 2945.841 kb on - strand, within leuC at 2945.841 kb on - strand, within leuC at 2945.841 kb on - strand, within leuC at 2945.841 kb on - strand, within leuC at 2945.841 kb on - strand, within leuC at 2945.841 kb on - strand, within leuC
Per-strain Table
Position Strand Gene LocusTag Fraction NLDM_defined_nocarbon with D-Glucose and L-Methionine 1 mM remove 2,943,067 + leuA OKGIIK_13575 0.51 -1.9 2,943,067 + leuA OKGIIK_13575 0.51 -3.0 2,943,068 - leuA OKGIIK_13575 0.51 -2.6 2,943,199 + leuA OKGIIK_13575 0.59 -2.0 2,943,199 + leuA OKGIIK_13575 0.59 -2.5 2,943,199 + leuA OKGIIK_13575 0.59 +0.0 2,943,200 - leuA OKGIIK_13575 0.59 -4.4 2,943,200 - leuA OKGIIK_13575 0.59 -1.3 2,943,200 - leuA OKGIIK_13575 0.59 -0.6 2,943,200 - leuA OKGIIK_13575 0.59 -2.8 2,943,200 - leuA OKGIIK_13575 0.59 +0.0 2,943,200 - leuA OKGIIK_13575 0.59 +1.0 2,943,200 - leuA OKGIIK_13575 0.59 -0.6 2,943,958 - -0.8 2,943,958 - -2.2 2,943,958 - -3.0 2,943,990 + ilvE OKGIIK_13580 0.12 +0.0 2,943,990 + ilvE OKGIIK_13580 0.12 -1.3 2,943,990 + ilvE OKGIIK_13580 0.12 -1.8 2,943,990 + ilvE OKGIIK_13580 0.12 -3.6 2,943,990 + ilvE OKGIIK_13580 0.12 +0.0 2,943,990 + ilvE OKGIIK_13580 0.12 -2.2 2,943,990 + ilvE OKGIIK_13580 0.12 -4.3 2,943,990 + ilvE OKGIIK_13580 0.12 -1.3 2,943,990 + ilvE OKGIIK_13580 0.12 -1.6 2,943,990 + ilvE OKGIIK_13580 0.12 +0.0 2,943,990 + ilvE OKGIIK_13580 0.12 -1.0 2,943,990 + ilvE OKGIIK_13580 0.12 +0.0 2,943,991 - ilvE OKGIIK_13580 0.12 -2.0 2,943,991 - ilvE OKGIIK_13580 0.12 -2.2 2,943,991 - ilvE OKGIIK_13580 0.12 +0.0 2,943,991 - ilvE OKGIIK_13580 0.12 -3.0 2,943,991 - ilvE OKGIIK_13580 0.12 -0.6 2,943,991 - ilvE OKGIIK_13580 0.12 -1.0 2,943,991 - ilvE OKGIIK_13580 0.12 -4.1 2,944,014 + ilvE OKGIIK_13580 0.14 -1.6 2,944,014 + ilvE OKGIIK_13580 0.14 -1.3 2,944,014 + ilvE OKGIIK_13580 0.14 -3.0 2,944,014 + ilvE OKGIIK_13580 0.14 -0.6 2,944,015 - ilvE OKGIIK_13580 0.14 -1.8 2,944,015 - ilvE OKGIIK_13580 0.14 -1.6 2,944,015 - ilvE OKGIIK_13580 0.14 -3.2 2,944,065 + ilvE OKGIIK_13580 0.19 -0.6 2,944,066 - ilvE OKGIIK_13580 0.19 -2.2 2,944,066 - ilvE OKGIIK_13580 0.19 -0.6 2,944,149 + ilvE OKGIIK_13580 0.28 -2.5 2,944,149 + ilvE OKGIIK_13580 0.28 -3.5 2,944,149 + ilvE OKGIIK_13580 0.28 -2.2 2,944,149 + ilvE OKGIIK_13580 0.28 -1.8 2,944,149 + ilvE OKGIIK_13580 0.28 -1.8 2,944,149 + ilvE OKGIIK_13580 0.28 -1.3 2,944,149 + ilvE OKGIIK_13580 0.28 -2.2 2,944,149 + ilvE OKGIIK_13580 0.28 -2.8 2,944,149 + ilvE OKGIIK_13580 0.28 -2.0 2,944,149 + ilvE OKGIIK_13580 0.28 -1.0 2,944,149 + ilvE OKGIIK_13580 0.28 -1.6 2,944,149 + ilvE OKGIIK_13580 0.28 -1.8 2,944,149 + ilvE OKGIIK_13580 0.28 +1.0 2,944,150 - ilvE OKGIIK_13580 0.28 -3.6 2,944,150 - ilvE OKGIIK_13580 0.28 +0.0 2,944,150 - ilvE OKGIIK_13580 0.28 -3.4 2,944,150 - ilvE OKGIIK_13580 0.28 -1.3 2,944,150 - ilvE OKGIIK_13580 0.28 -1.6 2,944,150 - ilvE OKGIIK_13580 0.28 -2.7 2,944,150 - ilvE OKGIIK_13580 0.28 -0.6 2,944,150 - ilvE OKGIIK_13580 0.28 -3.1 2,944,150 - ilvE OKGIIK_13580 0.28 -1.3 2,944,150 - ilvE OKGIIK_13580 0.28 -1.6 2,944,150 - ilvE OKGIIK_13580 0.28 +0.4 2,944,150 - ilvE OKGIIK_13580 0.28 -1.8 2,944,251 + ilvE OKGIIK_13580 0.38 -2.0 2,944,251 + ilvE OKGIIK_13580 0.38 -3.2 2,944,251 + ilvE OKGIIK_13580 0.38 -1.0 2,944,251 + ilvE OKGIIK_13580 0.38 -3.6 2,944,251 + ilvE OKGIIK_13580 0.38 -1.6 2,944,251 + ilvE OKGIIK_13580 0.38 -1.6 2,944,251 + ilvE OKGIIK_13580 0.38 -3.4 2,944,251 + ilvE OKGIIK_13580 0.38 +1.6 2,944,251 + ilvE OKGIIK_13580 0.38 -2.2 2,944,252 - ilvE OKGIIK_13580 0.38 +1.0 2,944,252 - ilvE OKGIIK_13580 0.38 +0.0 2,944,252 - ilvE OKGIIK_13580 0.38 -1.6 2,944,353 + ilvE OKGIIK_13580 0.48 -1.3 2,944,353 + ilvE OKGIIK_13580 0.48 -1.0 2,944,353 + ilvE OKGIIK_13580 0.48 +0.0 2,944,353 + ilvE OKGIIK_13580 0.48 -2.7 2,944,353 + ilvE OKGIIK_13580 0.48 +0.0 2,944,353 + ilvE OKGIIK_13580 0.48 -1.6 2,944,353 + ilvE OKGIIK_13580 0.48 -0.6 2,944,353 + ilvE OKGIIK_13580 0.48 -2.9 2,944,353 + ilvE OKGIIK_13580 0.48 -0.6 2,944,353 + ilvE OKGIIK_13580 0.48 -1.3 2,944,353 + ilvE OKGIIK_13580 0.48 -1.0 2,944,353 + ilvE OKGIIK_13580 0.48 -1.6 2,944,353 + ilvE OKGIIK_13580 0.48 +0.4 2,944,353 + ilvE OKGIIK_13580 0.48 -2.2 2,944,353 + ilvE OKGIIK_13580 0.48 -3.5 2,944,353 + ilvE OKGIIK_13580 0.48 -1.8 2,944,353 + ilvE OKGIIK_13580 0.48 -1.0 2,944,354 - ilvE OKGIIK_13580 0.48 -2.2 2,944,354 - ilvE OKGIIK_13580 0.48 -1.8 2,944,354 - ilvE OKGIIK_13580 0.48 -2.6 2,944,354 - ilvE OKGIIK_13580 0.48 -1.8 2,944,354 - ilvE OKGIIK_13580 0.48 -1.3 2,944,354 - ilvE OKGIIK_13580 0.48 -1.3 2,944,354 - ilvE OKGIIK_13580 0.48 -3.2 2,944,354 - ilvE OKGIIK_13580 0.48 -2.8 2,944,354 - ilvE OKGIIK_13580 0.48 -1.6 2,944,354 - ilvE OKGIIK_13580 0.48 -2.2 2,944,354 - ilvE OKGIIK_13580 0.48 -2.7 2,944,354 - ilvE OKGIIK_13580 0.48 -3.4 2,944,354 - ilvE OKGIIK_13580 0.48 -3.5 2,944,354 - ilvE OKGIIK_13580 0.48 -1.2 2,944,354 - ilvE OKGIIK_13580 0.48 -1.7 2,944,354 - ilvE OKGIIK_13580 0.48 -1.6 2,944,602 + ilvE OKGIIK_13580 0.73 -2.3 2,944,602 + ilvE OKGIIK_13580 0.73 -2.0 2,944,603 - ilvE OKGIIK_13580 0.73 -3.5 2,944,603 - ilvE OKGIIK_13580 0.73 -1.3 2,944,603 - ilvE OKGIIK_13580 0.73 -1.3 2,944,603 - ilvE OKGIIK_13580 0.73 -1.0 2,944,748 + ilvE OKGIIK_13580 0.87 -2.2 2,944,781 + -0.6 2,944,952 + -2.9 2,944,952 + -3.6 2,944,952 + +1.0 2,944,952 + -0.6 2,944,952 + +0.7 2,944,952 + -1.6 2,944,952 + -2.2 2,944,952 + -1.6 2,944,953 - -1.0 2,944,953 - -3.1 2,944,953 - -2.5 2,944,953 - -2.2 2,945,109 - leuC OKGIIK_13585 0.17 -1.0 2,945,446 + leuC OKGIIK_13585 0.41 -0.6 2,945,446 + leuC OKGIIK_13585 0.41 -3.0 2,945,446 + leuC OKGIIK_13585 0.41 -1.8 2,945,446 + leuC OKGIIK_13585 0.41 -2.8 2,945,446 + leuC OKGIIK_13585 0.41 -1.8 2,945,446 + leuC OKGIIK_13585 0.41 -1.3 2,945,446 + leuC OKGIIK_13585 0.41 -3.2 2,945,446 + leuC OKGIIK_13585 0.41 -2.2 2,945,446 + leuC OKGIIK_13585 0.41 -2.2 2,945,446 + leuC OKGIIK_13585 0.41 -2.3 2,945,446 + leuC OKGIIK_13585 0.41 -0.8 2,945,446 + leuC OKGIIK_13585 0.41 -1.6 2,945,446 + leuC OKGIIK_13585 0.41 -1.8 2,945,447 - leuC OKGIIK_13585 0.41 -2.3 2,945,447 - leuC OKGIIK_13585 0.41 -2.6 2,945,447 - leuC OKGIIK_13585 0.41 -2.2 2,945,447 - leuC OKGIIK_13585 0.41 -1.3 2,945,447 - leuC OKGIIK_13585 0.41 -1.3 2,945,447 - leuC OKGIIK_13585 0.41 -1.6 2,945,495 + leuC OKGIIK_13585 0.44 -1.8 2,945,495 + leuC OKGIIK_13585 0.44 -3.1 2,945,705 + leuC OKGIIK_13585 0.59 -2.2 2,945,706 - leuC OKGIIK_13585 0.59 +0.5 2,945,756 + leuC OKGIIK_13585 0.63 -1.0 2,945,756 + leuC OKGIIK_13585 0.63 -3.2 2,945,756 + leuC OKGIIK_13585 0.63 -3.3 2,945,756 + leuC OKGIIK_13585 0.63 -1.0 2,945,756 + leuC OKGIIK_13585 0.63 -2.5 2,945,757 - leuC OKGIIK_13585 0.63 -0.6 2,945,757 - leuC OKGIIK_13585 0.63 -4.1 2,945,757 - leuC OKGIIK_13585 0.63 -2.5 2,945,757 - leuC OKGIIK_13585 0.63 -1.3 2,945,758 - leuC OKGIIK_13585 0.63 -2.5 2,945,840 + leuC OKGIIK_13585 0.69 -2.2 2,945,840 + leuC OKGIIK_13585 0.69 -2.3 2,945,840 + leuC OKGIIK_13585 0.69 +0.0 2,945,840 + leuC OKGIIK_13585 0.69 -1.6 2,945,840 + leuC OKGIIK_13585 0.69 +2.0 2,945,840 + leuC OKGIIK_13585 0.69 -2.5 2,945,840 + leuC OKGIIK_13585 0.69 -1.0 2,945,840 + leuC OKGIIK_13585 0.69 -3.0 2,945,840 + leuC OKGIIK_13585 0.69 -3.4 2,945,840 + leuC OKGIIK_13585 0.69 -1.3 2,945,841 - leuC OKGIIK_13585 0.69 -1.8 2,945,841 - leuC OKGIIK_13585 0.69 -0.9 2,945,841 - leuC OKGIIK_13585 0.69 -1.6 2,945,841 - leuC OKGIIK_13585 0.69 -1.6 2,945,841 - leuC OKGIIK_13585 0.69 +1.0 2,945,841 - leuC OKGIIK_13585 0.69 +0.0 2,945,841 - leuC OKGIIK_13585 0.69 -0.6
Or see this region's nucleotide sequence